Exploring antioxidant activities and inhibitory effects against α‐amylase and α‐glucosidase of Elaeocarpus braceanus fruits: insights into mechanisms by molecular docking and molecular dynamics

International Journal of Food Science & Technology, Год журнала: 2023, Номер 59(1), С. 343 - 355

Опубликована: Ноя. 3, 2023

Summary The present work investigated the antioxidant activity and inhibitory effects towards α‐amylase α‐glucosidase of phenolic compounds in Elaeocarpus braceanus fruits clarified their possible mechanisms based on molecular docking dynamic analysis. A total 12 were identified quantified extracts E. using UHPLC‐ESI‐HRMS/MS. TPC TFC 229.18 ± 5.76 mg GAE/g 144.85 1.17 RE/g, respectively. In vitro analyses, exhibited good activities demonstrated strong α‐glucosidase. Molecular dynamics results showed that quercetin myricetin can stably form hydrogen bonds van der Waals forces with active amino acid residues α‐glucosidase, thereby inhibiting enzymatic activities. findings might provide a potential natural resource for improving oxidative stress damage controlling blood glucose diabetes.

Язык: Английский

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Current advancement in the preclinical models used for the assessment of diabetic neuropathy

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2023, Номер 397(5), С. 2727 - 2745

Опубликована: Ноя. 21, 2023

Язык: Английский

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Cell identity dynamics and insight into insulin secretagogues when employing stem cell-derived islets for disease modeling

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12

Опубликована: Июнь 12, 2024

Stem cell-derived islets (SC-islets) are not only an unlimited source for cell-based therapy of type 1 diabetes but have also emerged as attractive material modeling and conducting screening treatment options. Prior to SC-islets becoming the established standard disease drug development, it is essential understand their response various nutrient sources in vitro . This study demonstrates enhanced efficiency pancreatic endocrine cell differentiation through incorporation WNT signaling inhibition following definitive endoderm stage. We identified a tri-hormonal population within SC-islets, which undergoes reduction concurrent with emergence elevated numbers glucagon-positive cells during extended culture. Over 6-week period culture, consistently demonstrated robust insulin secretion glucose stimulation. Moreover, they manifested diverse reactivity patterns when exposed distinct exhibited deviant glycolytic metabolic characteristics comparison human primary islets. Although aberrant metabolism trafficking, evaluation potential antidiabetic drug, pyruvate kinase agonist known TEPP46, significantly improved SC-islets. Overall, this provided identity dynamics investigation prolonged culturing , insights into secretagogues. Associated advantages limitations were discussed employing modeling.

Язык: Английский

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Historic obstacles and emerging opportunities in the field of developmental metabolism – lessons from Heidelberg

Development, Год журнала: 2024, Номер 151(12)

Опубликована: Июнь 15, 2024

The field of developmental metabolism is experiencing a technological revolution that opening entirely new fields inquiry. Advances in metabolomics, small-molecule sensors, single-cell RNA sequencing and computational modeling present opportunities for exploring cell-specific tissue-specific metabolic networks, interorgan communication, gene-by-metabolite interactions time space. Together, these advances not only means by which biologists can tackle questions have challenged the centuries, but also young scientists with to define areas These emerging frontiers were at center highly interactive 2023 EMBO workshop 'Developmental metabolism: flows energy, matter, information'. Here, we summarize key discussions from this forum, emphasizing modern biology's challenges opportunities.

Язык: Английский

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Beyond skin deep: Revealing the essence of iPS cell-generated skin organoids in regeneration

Burns, Год журнала: 2024, Номер unknown, С. 107194 - 107194

Опубликована: Июнь 1, 2024

Язык: Английский

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