Comparative Analysis of Letrozole and Estradiol Valerate PCOS Models: Reproductive and Metabolic Outcomes with and Without High-Fat Diet
Biology,
Год журнала:
2025,
Номер
14(6), С. 592 - 592
Опубликована: Май 23, 2025
Polycystic
ovary
syndrome
(PCOS)
is
a
prevalent
endocrine
disorder
in
reproductive-aged
women,
characterized
by
hyperandrogenism,
oligoanovulation,
and
polycystic
ovarian
morphology.
Despite
its
classification
as
reproductive
disorder,
PCOS
closely
associated
with
metabolic
dysregulation,
including
insulin
resistance
obesity.
An
ideal
animal
model
for
should
replicate
both
features
of
the
condition.
In
this
study,
we
compared
two
widely
used
postnatal
models
(letrozole
estradiol
valerate
[EV])
administered
alone
or
combination
high-fat
diet
(HFD),
assessing
their
ability
to
induce
features.
Letrozole
treatment
led
significant
weight
gain
increased
visceral
adiposity,
effects
that
were
amplified
HFD.
Conversely,
EV
showed
tendency
toward
reduced
body
mass.
While
neither
significantly
altered
fasting
glucose
levels,
letrozole
combined
HFD
impaired
tolerance,
supporting
role
dysfunction.
Hyperandrogenism
was
more
consistently
induced
EV,
aligning
clinical
phenotypes.
Both
treatments
disrupted
estrous
cyclicity
morphology,
though
disturbances
pronounced
model.
These
findings
suggest
letrozole,
particularly
HFD,
provides
consistent
studying
facets
PCOS.
Язык: Английский
Androgens and Hirsutism in a Large Cohort of Portuguese Women
Journal of Clinical Medicine,
Год журнала:
2025,
Номер
14(3), С. 673 - 673
Опубликована: Янв. 21, 2025
Background/Objectives:
Hirsutism
is
excessive
male-patterned
hair
in
postpubertal
women
with
multifactorial
etiology
and
an
indicator
of
hyperandrogenism
associated
polycystic
ovary
syndrome
(PCOS).
Indeed,
it
can
be
caused
by
the
enhanced
peripheral
conversion
androgen
precursors
to
testosterone,
as
idiopathic
hirsutism
(IH).
Moreover,
hirsutism-related
hyperandrogenic
syndromes
like
non-classic
congenital
adrenal
hyperplasia
(NCAH)
(IHA).
Methods:
In
this
study,
we
characterized
a
large
cohort
Portuguese
referred
for
estimated
prevalence
PCOS,
NCAH,
IHA,
IH.
The
levels
androgens
gonadotropins
body
mass
index
(BMI)
were
measured
compared
controls.
correlation
between
each
variable
was
calculated.
Results:
cohort,
found
PCOS
56.2%,
IH
20.2%,
IHA
17.3%,
NCAH
6.2%.
Subjects
only
ones
showing
significant
difference
BMI
controls
had
lowest
sex
hormone-binding
globulin
(SHBG).
Those
younger
more
hirsute
higher
among
other
androgens.
lower
luteinizing
hormone
(LH)
LH/follicle-stimulating
(FSH)
ratios
than
those
PCOS.
SHBG
free
(FAI).
IH,
particular,
adrenal-derived
Conclusions:
pathogenesis
complex,
contributions
pituitary
gland,
ovaries,
adrenals,
adipose
tissue,
liver
have
ascertained
understand
clinical
manifestations
delineate
appropriate
treatments.
This
study
sheds
new
light
on
fine
hormonal
regulation
these
diseases.
Язык: Английский
Gut Microbiota Modulation by Inulin Improves Metabolism and Ovarian Function in Polycystic Ovary Syndrome
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 7, 2025
Abstract
The
management
of
metabolic
disorder
associated
with
polycystic
ovary
syndrome
(PCOS)
has
been
suggested
as
an
effective
approach
to
improve
PCOS
which
is
highly
involved
gut
microbiota,
while
the
underlying
mechanism
unclear.
Here,
we
investigated
role
inulin,
a
microbiota
regulator,
in
alleviation
PCOS.
Our
findings
showed
that
inulin
treatment
significantly
improved
hyperandrogenism
and
glucolipid
metabolism
both
cohort
mice.
Consistent
cohort,
increased
abundance
microbial
co‐abundance
group
(CAG)
12
mice,
including
Bifidobacterium
species
other
short‐chain
fatty
acids
(SCFAs)‐producers.
We
further
verified
enhancement
SCFAs
biosynthesis
capacity
fecal
content
by
inulin.
Moreover,
decreased
lipopolysaccharide‐binding
protein
(LBP)
ameliorated
ovarian
inflammation
whereas
intraperitoneal
lipopolysaccharide
(LPS)
administration
reversed
protective
effects
Furthermore,
transplantation
(FMT)
from
inulin‐treated
patients
enhanced
insulin
sensitivity,
lipid
accumulation
thermogenesis,
reduced
inflammatory
response
antibiotic‐treated
Collectively,
these
revealed
mediates
beneficial
on
dysfunction
Therefore,
modulating
represents
promising
therapeutic
strategy
for
Язык: Английский
Distribution of polycystic ovary syndrome (PCOS) phenotypes in Iranian women: a cross-sectional study
BMC Research Notes,
Год журнала:
2025,
Номер
18(1)
Опубликована: Май 13, 2025
Polycystic
ovary
syndrome
(PCOS)
is
a
heterogeneous
disorder
characterized
by
diverse
clinical
and
metabolic
manifestations.
This
study
aimed
to
investigate
the
prevalence
of
PCOS
phenotypes
their
association
with
hematological,
biochemical,
hormonal
parameters
in
PCOS,
particular
focus
on
infertile
women
those
recurrent
pregnancy
loss
(RPL).
Phenotype
A
was
most
prevalent
phenotype
overall
within
both
RPL
subgroups.
However,
no
significant
differences
or
were
observed
among
phenotypes,
except
for
lower
RBC
hematocrit
levels
F.
demonstrate
significantly
RBC,
hemoglobin,
Язык: Английский
Androgen Receptor PROTAC ARV-110 Ameliorates Metabolic Complications in a Mouse Model of Polycystic Ovary Syndrome
Endocrinology,
Год журнала:
2025,
Номер
166(7)
Опубликована: Май 19, 2025
Abstract
Polycystic
ovary
syndrome
(PCOS)
is
the
most
common
endocrine
disorder
in
reproductive-age
women.
Hyperandrogenemia
(HA)
a
hallmark
of
PCOS
and
positively
associated
with
metabolic
complications.
Androgens
exert
their
biological
actions
through
androgen
receptor
(AR),
which
regulates
transcriptional
activity.
Antiandrogens
are
not
recommended
for
managing
complications
due
to
hepatotoxicity,
despite
being
viable
therapy
treat
HA.
We
hypothesized
that
novel
AR
Proteolysis
Targeting
Chimera
(PROTAC)
degrader
ARV-110
would
downregulate
protein
levels
abolish
or
mitigate
HA-mediated
using
well-established
HA
mouse
model
PCOS.
Three-week-old
female
mice
were
implanted
dihydrotestosterone
(DHT)
control
pellets.
Four
weeks
later,
treated
low-
(ARV-110-L,
1
mg/kg.day)
high-dose
(ARV-110-H,
10
an
additional
8
weeks.
dose-dependently
reduced
white
adipose
tissue
(WAT),
kidney,
liver,
ovary.
attenuated
DHT-induced
increases
body
weight,
fat
mass,
kidney
WAT
circulating
leptin
antimüllerian
hormone,
altered
glucose
homeostasis.
ARV-110-H
increased
(UACR,
KIM-1,
NGAL)
liver
(ALT,
AST,
LDH)
injury
markers
caused
severe
hepatomegaly,
while
ARV-110-L
mostly
spared
those
deleterious
effects.
Unbiased
proteomics
analysis
revealed
treatment
severely
affected
proteome
dysregulated
multiple
signaling
canonical
pathways,
only
minimal
effects
observed
treatment.
In
summary,
our
findings
underscore
potential
PROTACs
as
therapeutic
approach
PCOS,
provided
dosing
carefully
optimized
avoid
adverse
Язык: Английский
Assessment of Ovarian Stiffness in Women With Polycystic Ovary Syndrome
Journal of Ultrasound in Medicine,
Год журнала:
2025,
Номер
unknown
Опубликована: Июнь 2, 2025
Objective
To
evaluate
ovarian
stiffness
in
polycystic
ovary
syndrome
(PCOS)
patients
with
and
without
metabolic
(MetS)
using
acoustic
pulse
imaging.
Methods
This
case–control
study
was
conducted
a
tertiary
center
PCOS
outpatient
clinic.
A
total
of
105
participants,
51
(20
MetS
31
non‐MetS)
54
healthy
women
between
the
ages
20
35
years,
were
included
study.
Laboratory
sonographic
assessments
performed
early
follicular
phase.
The
shear
wave
elastography
(SWE)
technique
used
to
measure
tissue
transvaginally.
Results
Age
body
mass
index
found
be
similar
control
groups.
Mean
SWE
values
13.61
±
2.2
kPa
8.82
1.62
kPa,
groups,
respectively;
P
<
0.001.
intraobserver
intraclass
correlation
coefficient
value
for
mean
measurement
(kPa)
0.811
(good
reliability
agreement).
Using
receiver
operating
curve
analysis,
an
optimized
cut‐off
point
SWE_mean
10.58
determined.
Similar
subgroups
clinical
and/or
biochemical
hyperandrogenism.
In
MetS,
15.68
1.36
12.28
1.50
respectively
(
0.001).
moderate
positive
waist
circumference,
triglyceride
insulin
resistance
parameters,
weak
systolic
diastolic
blood
pressure.
Conclusion
Ovulatory
dysfunction
fibroinflammatory
environment
lead
quantitatively
measurable
changes
elasticity.
exacerbates
these
changes.
Ovarian
elasticity
associated
laboratory
markers.
Язык: Английский
Plumbagin ameliorates ferroptosis of ovarian granulosa cells in polycystic ovary syndrome by down-regulating SLC7A5 m6A methylation modification through inhibition of YTHDF1
Journal of Ovarian Research,
Год журнала:
2025,
Номер
18(1)
Опубликована: Июнь 2, 2025
Polycystic
ovary
syndrome
(PCOS)
is
a
common
endocrine-metabolic
disease
in
women
of
reproductive
age.
One
its
core
pathologies
ovarian
granulosa
cell
(GC)
dysfunction,
and
ferroptosis,
as
novel
death
mode
dependent
on
iron
ions
lipid
peroxidation,
may
be
involved
the
PCOS
process,
but
exact
mechanism
unknown.
Plumbagin
(PLB)
shows
potential
treatment
due
to
antioxidant
properties.
The
present
study
aimed
elucidate
molecular
mechanisms
by
which
PLB
ameliorates
mitochondrial
dysfunction
ferroptosis
GCs
through
YTH
N6-methyladenosine
RNA
binding
protein
1/L-type
amino
acid
transporter
1
(YTHDF1/SLC7A5)
axis.
An
vitro
model
was
constructed
treating
KGN
cells
with
dihydrotestosterone
(DHT),
treatment,
YTHDF1
knockdown
(si-YTHDF1),
SLC7A5
overexpression
(pcDNA
3.1-SLC7A5)
were
intervened
respectively.
Cell
viability
measured
counting
kit-8.
Lactate
dehydrogenase
(LDH)
release,
adenosine
triphosphate
(ATP)
level,
ion,
peroxidation
(LPO)
content
detected
commercial
kits.
Mitochondrial
membrane
(MMP)
analyzed
JC-1
staining
combined
flow
cytometry.
Reactive
oxygen
species
(ROS)
levels
assessed
C11-BODIPY
probe,
oxidative
stress
indicators
including
malondialdehyde
(MDA),
superoxide
dismutase
(SOD),
glutathione
peroxidase
kits,
Cytochrome
C,
Ferritin,
transcription
factor
A
(TFAM),
4
(GPX4)
expression
Western
blot.
Fluorescence
situ
hybridization,
immunoprecipitation,
m6A
quantitative
real-time
polymerase
chain
reaction
verified
interaction
translational
regulation
SLC7A5.
DHT
significantly
decreased
viability,
MMP
ATP
levels,
increased
LDH
ROS,
MDA,
LPO
content,
up-regulated
C
expression,
down-regulated
TFAM,
GPX4
expression.
Both
reversed
above
changes,
protective
effect
PLB.
co-localized
mRNA
enhanced
translation
modification.
reduced
without
affecting
weakened
knockdown,
resulting
deterioration
function,
ferroptosis.
DHT-induced
inhibiting
action
regulates
Downregulating
or
enhances
GC
survival
function.
Язык: Английский