Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102640 - 102640
Published: March 1, 2025
Language: Английский
Nature Reviews Molecular Cell Biology, Journal Year: 2025, Volume and Issue: unknown
Published: March 7, 2025
Language: Английский
Citations
5
Plant Cell Reports, Journal Year: 2025, Volume and Issue: 44(1)
Published: Jan. 1, 2025
Language: Английский
Citations
2
Sustainability, Journal Year: 2024, Volume and Issue: 16(14), P. 6026 - 6026
Published: July 15, 2024
As valuable sources of plant-based protein, leguminous vegetables (grain legumes) are essential for global food security and contribute to body growth development in humans as well animals. Climate change is a major challenge agriculture that creates problems the plants. However, legume productivity threatened by climate factors, including rising temperatures, shifting precipitation patterns, increased atmospheric carbon dioxide levels, intensified extreme events, altered pest/pathogen activity. This review synthesizes approximately 136 studies assess effects on crops. Under all emissions trajectories, mean temperatures projected rise beyond optimal growing thresholds 2050, carrying yield reductions between 10 49% beans, soybeans, cowpeas, lentils without adaptation measures. The elevated may transiently enhance yields up 18%, but benefits dramatically decline above 550 ppm cannot offset other impacts. Altered rainfall along with recurrent drought heat waves also expected decrease crop yields, seed quality, soil nitrogen levels worldwide. Furthermore, proliferation pests fungal diseases poses significant risks, amplified shifts 84% reviewed studies. These multifaceted impacts threaten gains sustainably meeting protein demand. Realizing resilience will require accelerated heat/drought-tolerant varieties, enhanced climate-informed agronomic practices, strong policy interventions, social safety nets explicitly supporting producers, addition policies/steps governments taking address challenges crisis. highlights adaptations mechanisms required crops thrive fulfill their roles nutrition. It explores how these can be improved better withstand environmental stresses, nutritional profiles, increase yields. Additionally, discusses importance legumes sustainable security, emphasizing potential future feeding population. By focusing critical aspects, aims underscore ensuring healthy supply.
Language: Английский
Citations
9
Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 28, 2024
ABSTRACT The past decade has witnessed rapid developments in gene discovery, biological big data (BBD), artificial intelligence (AI)‐aided technologies, and molecular breeding. These advancements are expected to accelerate crop breeding under the pressure of increasing demands for food. Here, we first summarize current methods discuss need new ways support efforts. Then, review how combine BBD AI technologies genetic dissection, exploring functional genes, predicting regulatory elements domains, phenotypic prediction. Finally, propose concept intelligent precision design (IPDB) driven by technology offer ideas about implement IPDB. We hope that IPDB will enhance predictability, efficiency, cost compared with technologies. As an example IPDB, explore possibilities offered CropGPT, which combines techniques, bioinformatics, art from breeders, presents open, shareable, cooperative system. provides integrated services communication platforms biologists, bioinformatics experts, germplasm resource specialists, dealers, farmers, should be well suited future
Language: Английский
Citations
7
Current Plant Biology, Journal Year: 2025, Volume and Issue: unknown, P. 100433 - 100433
Published: Jan. 1, 2025
Language: Английский
Citations
1
aBIOTECH, Journal Year: 2025, Volume and Issue: 6(1), P. 81 - 90
Published: Feb. 10, 2025
Abstract CRISPR/Cas is a simple, robust, versatile tool for plant biology studies and precision breeding. However, establishing high-efficiency gene editing system multiplex of the autotetraploid crop alfalfa ( Medicago sativa L.), most important forage legume worldwide, remains formidable challenge. Here, we systematically identified endogenous U6 promoters in through transient expression via Agrobacterium -mediated infiltration leaves. We further demonstrated efficacy three active genome using an optimized hairy root system. Subsequently, established improved CRISPR/Cas9 containing or four tandemly arrayed MsU6 -promoter-driven polycistronic tRNA-sgRNA (PTG) cassettes, each consisting units, to simultaneously edit genes, coupled with visual reporter RH1 RUBY . This toolkit showed efficient selection. successfully obtained regenerated, red-colored shoots resulting from stable transformation alfalfa. These results highlight potential application Our enables convenient, alfalfa, providing toolset facilitate functional multiple genes families basic research genetic improvement
Language: Английский
Citations
1
Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 10, 2025
Summary Transgene‐free genome editing is important for crop improvement as it reduces unanticipated genomic changes. While mRNA delivery systems offer a powerful method achieving transgene‐free editing, they remain inefficient and challenging in plants. Here we describe an efficient system plants with substantially improved efficiency. By optimizing the 5′ untranslated regions (5′UTRs) poly(A) tails of vitro ‐transcribed (IVT) mRNAs coating protamine during particle bombardment, have developed optimized termed v2_TMV/DEN2. This enhanced efficiencies knock‐out, A‐to‐G C‐to‐T base by average 4.7‐, 3.4‐ 2.5‐fold at various endogenous sites compared plasmid‐based transient via bombardment rice suspension cells wheat immature embryos 48 h post‐transformation. Furthermore, obtained edited 5.0–180.8% 26.1–26.2% using v2_TMV/DEN2 wheat, respectively, 0.0–43.2% 4.7–10.4% plasmids. Our study provides convenient
Language: Английский
Citations
1
Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 12, 2025
Summary The integration of haploid induction and genome editing, termed HI‐Edit/IMGE, is a promising tool for generating targeted mutations crop breeding. However, the technical components stacking suitable maize seed industry have yet to be fully characterised tested. Here, we developed assessed three HI‐Edit/IMGE lines: Edit Wx , Sh & using inducer CHOI3 lines engineered CRISPR‐Cas9 system targeting Waxy1 ( Wx1 ) Shrunken2 Sh2 genes. We meticulously systems, focusing on copy numbers mutant alleles mtl dmp which facilitate induction. Using B73 six other parental major commercial varieties as recipients, demonstrated maternal efficiencies ranging from 8.55% 20.89% mutation rates between 0.38% 1.46%. Comprehensive assessment verified identification, target gene editing accuracy, background integrity, related agronomic traits. Notably, successfully combined distinct systems induce multiple desired mutations, highlighting potential in accelerating edited traits into varieties. Our findings underscore importance meticulous Cas9 number characterisation highlight challenges somatic chimerism. also validated performance single‐cross haploids derived process. results confirm industrial applicability through pollination provide critical insights further optimising this technology.
Language: Английский
Citations
1
The Plant Journal, Journal Year: 2025, Volume and Issue: 121(4)
Published: Feb. 1, 2025
SUMMARY Soybean ( Glycine max Merr.) is the world's most important oilseed crop and its ability to fix atmospheric nitrogen makes it a cornerstone of sustainable agriculture. Despite importance, Europe relies heavily on imports, leading environmental economic vulnerabilities. To address these challenges, European Union has implemented policies boost local soybean production, emphasizing practices reduced dependency imports. However, conventional breeding methods are time‐consuming may not keep pace with rapid consumer habit changes. Genome‐editing technologies, such as CRISPR‐Cas, offer precise efficient tools for developing varieties tailored conditions. These technologies can enhance traits related precocity, stress responses, yield quality that essential adapting climate change promoting ecological sustainability. This review explores integration genome editing (GE) in breeding, highlighting potential advancing agroecological transition Europe. By having clear regulation enhancing efforts, GE significantly contribute resilient varieties, fostering competitive environmentally friendly
Language: Английский
Citations
1
Vegetable Research, Journal Year: 2025, Volume and Issue: 5(1), P. 0 - 0
Published: Jan. 1, 2025
Language: Английский
Citations
1