Molecular Cell, Год журнала: 2024, Номер 84(19), С. 3596 - 3609
Опубликована: Окт. 1, 2024
Язык: Английский
Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Апрель 11, 2025
Double-stranded RNA (dsRNA) binding proteins (dsRBPs) play crucial roles in various cellular processes, especially the innate immune response. Comprehensive characterization of dsRBPs is essential to understand intricate mechanisms for dsRNA sensing and Traditional methods have predominantly relied on affinity purification, favoring isolation strong binders. Here, we adopt proteome integral solubility alteration (PISA) workflow characterizing dsRBPs, resulting observation 18 known identification 200 potential dsRBPs. Next, focus zinc finger protein 385 A (ZNF385A) discover that its knockout activates transcription interferon-β absence immunogenic stimuli. The ZNF385A elevates level endogenous dsRNAs, transcripts associated with retroelements, such as short interspersed nuclear element (SINE), long (LINE), terminal repeat (LTR). Moreover, loss enhances bioactivity 5-Aza-2'-deoxycytidine (5-AZA-CdR) tumor-killing effect NK cells. Our findings greatly expand dsRBP reservoir contribute understanding homeostasis.
Язык: Английский
Процитировано
0
Nature Reviews Genetics, Год журнала: 2025, Номер unknown
Опубликована: Апрель 14, 2025
Язык: Английский
Процитировано
0
Molecular Cell, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
World Journal of Clinical Cases, Год журнала: 2025, Номер 13(21)
Опубликована: Апрель 26, 2025
Pleural effusion, characterized by the accumulation of fluid in pleural space, poses significant challenges clinical practice, especially determining whether it belongs to inflammatory exudates or non-inflammatory transudates. Adenosine deaminase (ADA), an enzyme primarily produced immune cells, particularly lymphocytes, increase response conditions, including tuberculosis and malignancies. Elevated ADA levels have been shown correlate with exudates, making a valuable biomarker for differentiating between effusions. Moreover, numerous studies demonstrated treatment function inflammation- related effusion syndrome. Recently, research has established values implication diagnosing managing disease. Based on these findings, becomes reliable, non-invasive marker early diagnosis appropriate inflammation, ultimately improving patient outcomes.
Язык: Английский
Процитировано
0
Viruses, Год журнала: 2024, Номер 16(6), С. 945 - 945
Опубликована: Июнь 12, 2024
The genomes of positive-sense (+) single-stranded RNA (ssRNA) viruses are believed to be subjected a wide range modifications. In this study, we focused on the chikungunya virus (CHIKV) as model ssRNA study landscape viral modification in infected human cells. Among 32 distinct modifications analysed by mass spectrometry, inosine was found enriched genomic CHIKV RNA. However, orthogonal validation Illumina RNA-seq analyses did not identify any along genome. Moreover, infection alter expression ADAR1 isoforms, enzymes that catalyse adenosine conversion. Together, highlights importance multidisciplinary approach assess presence genomes.
Язык: Английский
Процитировано
2
Nature Immunology, Год журнала: 2024, Номер 25(8), С. 1322 - 1325
Опубликована: Июль 15, 2024
Язык: Английский
Процитировано
2
Molecular Therapy — Nucleic Acids, Год журнала: 2024, Номер 35(3), С. 102284 - 102284
Опубликована: Июль 20, 2024
Язык: Английский
Процитировано
2
Biomedicines, Год журнала: 2024, Номер 12(8), С. 1728 - 1728
Опубликована: Авг. 2, 2024
The innate immune system is the body's natural defense system, which recognizes a wide range of microbial molecules (such as bacterial DNA and RNA) abnormal within cells misplaced DNA, self-antigens) to play its role. released into cytoplasm activates cyclic GMP-AMP synthase (cGAS)-stimulator interferon genes (STING) signaling pathway initiate an response. Ischemia-reperfusion injury (IRI) after acute myocardial infarction refers phenomenon where tissue suffers further damage upon restoration blood flow. This issue significant clinical problem in treatment infarction, it can diminish effectiveness reperfusion therapy lead deterioration cardiac function. Studies have found that cGAS-STING closely related this phenomenon. Therefore, review aims describe role ischemia-reperfusion summarize current development status inhibitors application nanomaterials elucidate potential therapeutic target.
Язык: Английский
Процитировано
2
Biochemistry, Год журнала: 2024, Номер 63(6), С. 777 - 787
Опубликована: Март 4, 2024
The left-handed Z-conformation of nucleic acids can be adopted by both DNA and RNA when bound Zα domains found within a variety viral innate immune response proteins. While Z-form adoption is preferred certain sequences, such as the commonly studied (CpG)n repeats, has been reported to bind wide range sequence contexts. Studying how interacts with B-/A-form helices prior their conversion challenging binding coincides adoption. Here, we fromHomo sapiens ADAR1 locked "A-type" version (CpG)3 construct (LNA (CpG)3) where sugar pucker into C3′-endo/C2′-exo conformation, which prevents duplex from adopting alternating C2′/C3′-endo puckers in Z-conformation. Using NMR other biophysical techniques, find that ZαADAR1 binds LNA using similar interface for binding, dissociation constant (KD) ∼4 μM. In contrast Z-DNA/Z-RNA, two every 6 bp stretch, our data suggests multiple molecules, indicating completely different mode. Because relatively tightly non-Z-form model, its B/A-form may need considered experiments are carried out attempt identify targets domains. use constructs beneficial negative controls needed.
Язык: Английский
Процитировано
1
Advances in immunology, Год журнала: 2024, Номер unknown, С. 53 - 83
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
1