Journal of the American Chemical Society,
Год журнала:
2022,
Номер
144(10), С. 4667 - 4677
Опубликована: Март 7, 2022
Constructing
artificial
dynamic
architectures
inside
cells
to
rationally
interfere
with
organelles
is
emerging
as
an
efficient
strategy
regulate
the
behaviors
and
fate
of
cells,
thus
providing
new
routes
for
therapeutics.
Herein,
we
develop
intracellular
K+-mediating
assembly
DNA
tetrahedrons
which
realizes
mitochondrial
interference
consequent
regulation
on
energy
metabolism
living
cells.
In
designer
tetrahedron,
one
vertex
was
modified
triphenylphosphine
(TPP)
targeting,
other
three
vertexes
were
tethered
guanine-rich
sequences
that
could
realize
formation
intermolecular
G-quadruplexes,
consequently
led
form
aggregates
in
cytoplasm.
The
specially
targeted
mitochondria
served
a
polyanionic
barrier
substance
communication,
generating
significant
inhibition
effect
aerobic
respiration
function
associated
glycolysis
process,
reduced
production
adenosine
triphosphate
(ATP).
lack
ATP
impeded
lamellipodium
essential
movement
resulting
inhibitory
cell
migration.
Remarkably,
migration
capacity
suppressed
by
high
50%
cancer
This
work
provides
manipulation
via
endogenous
molecule-mediating
exogenous
envisioned
have
great
potential
precise
biomedicine.
Review30
August
2021Open
Access
Autophagy
in
major
human
diseases
Daniel
J
Klionsky
orcid.org/0000-0002-7828-8118
Life
Sciences
Institute,
University
of
Michigan,
Ann
Arbor,
MI,
USA
Search
for
more
papers
by
this
author
Giulia
Petroni
Department
Radiation
Oncology,
Weill
Cornell
Medical
College,
New
York,
NY,
Ravi
K
Amaravadi
Medicine,
Pennsylvania,
Philadelphia,
PA,
Abramson
Cancer
Center,
Eric
H
Baehrecke
Molecular,
Cell
and
Biology,
Massachusetts
School,
Worcester,
MA,
Andrea
Ballabio
orcid.org/0000-0003-1381-4604
Telethon
Institute
Genetics
Pozzuoli,
Italy
Translational
Sciences,
Section
Pediatrics,
Federico
II
University,
Naples,
Molecular
Human
Genetics,
Baylor
College
Jan
Dan
Duncan
Neurological
Research
Texas
Children
Hospital,
Houston,
TX,
Patricia
Boya
orcid.org/0000-0003-3045-951X
Margarita
Salas
Center
Biological
Research,
Spanish
National
Council,
Madrid,
Spain
José
Manuel
Bravo-San
Pedro
Faculty
Physiology,
Complutense
Networked
Biomedical
Neurodegenerative
Diseases
(CIBERNED),
Ken
Cadwell
Kimmel
Biology
Medicine
at
the
Skirball
York
Grossman
School
Microbiology,
Division
Gastroenterology
Hepatology,
Langone
Health,
Francesco
Cecconi
orcid.org/0000-0002-5614-4359
Stress
Survival
Unit,
Autophagy,
Recycling
Disease
(CARD),
Danish
Society
Copenhagen,
Denmark
Pediatric
Onco-Hematology
Gene
Therapy,
IRCCS
Bambino
Gesù
Children's
Rome,
Rome
'Tor
Vergata',
Augustine
M
Choi
Pulmonary
Critical
Care
Joan
Sanford
I.
York-Presbyterian
Mary
E
Nephrology
Hypertension,
Charleen
T
Chu
orcid.org/0000-0002-5052-8271
Pathology,
Pittsburgh
Pittsburgh,
Patrice
Codogno
orcid.org/0000-0002-5492-3180
Institut
Necker-Enfants
Malades,
INSERM
U1151-CNRS
UMR
8253,
Paris,
France
Université
de
Maria
Isabel
Colombo
Laboratorio
Mecanismos
Moleculares
Implicados
en
el
Tráfico
Vesicular
y
la
Autofagia-Instituto
Histología
Embriología
(IHEM)-Universidad
Nacional
Cuyo,
CONICET-
Facultad
Ciencias
Médicas,
Mendoza,
Argentina
Ana
Cuervo
orcid.org/0000-0002-0771-700X
Developmental
Albert
Einstein
Bronx,
Aging
Studies,
Vojo
Deretic
Inflammation
Metabolism
(AIM,
Excellence,
Mexico
Health
Albuquerque,
NM,
Ivan
Dikic
orcid.org/0000-0001-8156-9511
Biochemistry
II,
Goethe
Frankfurt,
Frankfurt
am
Main,
Germany
Buchmann
Zvulun
Elazar
Biomolecular
The
Weizmann
Science,
Rehovot,
Israel
Eeva-Liisa
Eskelinen
Biomedicine,
Turku,
Finland
Gian
Fimia
orcid.org/0000-0003-4438-3325
Sapienza
Epidemiology,
Preclinical
Advanced
Diagnostics,
Infectious
'L.
Spallanzani'
IRCCS,
David
A
Gewirtz
orcid.org/0000-0003-0437-4934
Pharmacology
Toxicology,
Virginia
Commonwealth
Richmond,
VA,
Douglas
R
Green
Immunology,
St.
Jude
Memphis,
TN,
Malene
Hansen
Burnham
Prebys
Discovery
Program
Development,
Aging,
Regeneration,
La
Jolla,
CA,
Marja
Jäättelä
orcid.org/0000-0001-5950-7111
Death
Metabolism,
&
Disease,
Cellular
Terje
Johansen
orcid.org/0000-0003-1451-9578
Group,
Tromsø—The
Arctic
Norway,
Tromsø,
Norway
Gábor
Juhász
Szeged,
Hungary
Anatomy,
Eötvös
Loránd
Budapest,
Vassiliki
Karantza
Merck
Co.,
Inc.,
Kenilworth,
NJ,
Claudine
Kraft
orcid.org/0000-0002-3324-4701
ZBMZ,
Freiburg,
CIBSS
-
Centre
Integrative
Signalling
Guido
Kroemer
orcid.org/0000-0002-9334-4405
Recherche
des
Cordeliers,
Equipe
Labellisée
par
Ligue
Contre
le
Cancer,
Sorbonne
Université,
Inserm
U1138,
Universitaire
France,
Metabolomics
Platforms,
Gustave
Roussy,
Villejuif,
Pôle
Biologie,
Hôpital
Européen
Georges
Pompidou,
AP-HP,
Suzhou
Systems
Chinese
Academy
Suzhou,
China
Karolinska
Women's
Stockholm,
Sweden
Nicholas
Ktistakis
Programme,
Babraham
Cambridge,
UK
Sharad
Kumar
orcid.org/0000-0001-7126-9814
South
Australia,
Adelaide,
SA,
Australia
Carlos
Lopez-Otin
orcid.org/0000-0001-6964-1904
Departamento
Bioquímica
Biología
Medicina,
Instituto
Universitario
Oncología
del
Principado
Asturias
(IUOPA),
Universidad
Oviedo,
Centro
Investigación
Biomédica
Red
Cáncer
(CIBERONC),
Kay
F
Macleod
Ben
May
Gordon
W-338,
Chicago,
IL,
Frank
Madeo
Biosciences,
NAWI
Graz,
Austria
BioTechMed-Graz,
Field
Excellence
BioHealth
–
Jennifer
Martinez
Immunity,
Laboratory,
Environmental
NIH,
Triangle
Park,
NC,
Alicia
Meléndez
Department,
Queens
City
Flushing,
Graduate
PhD
Programs
Noboru
Mizushima
orcid.org/0000-0002-6258-6444
Tokyo,
Japan
Christian
Münz
orcid.org/0000-0001-6419-1940
Viral
Immunobiology,
Experimental
Zurich,
Switzerland
Josef
Penninger
Biotechnology
Austrian
(IMBA),
Vienna
BioCenter
(VBC),
Vienna,
British
Columbia,
Vancouver,
BC,
Canada
Rushika
Perera
orcid.org/0000-0003-2435-2273
California,
San
Francisco,
Helen
Diller
Family
Comprehensive
Mauro
Piacentini
orcid.org/0000-0003-2919-1296
"Tor
Vergata",
Laboratory
Cytology
Russian
Saint
Petersburg,
Russia
Fulvio
Reggiori
orcid.org/0000-0003-2652-2686
Cells
Systems,
Section,
Groningen,
Netherlands
C
Rubinsztein
Cambridge
Dementia
Kevin
Ryan
Beatson
Glasgow,
Junichi
Sadoshima
Cardiovascular
Rutgers
Jersey
Newark,
Laura
Santambrogio
Sandra
Edward
Meyer
Caryl
Englander
Precision
Luca
Scorrano
orcid.org/0000-0002-8515-8928
Istituto
Veneto
di
Medicina
Molecolare,
Padova,
Hans-Uwe
Simon
Pharmacology,
Bern,
Clinical
Immunology
Allergology,
Sechenov
Moscow,
Fundamental
Kazan
Federal
Kazan,
Anna
Katharina
Kennedy
Rheumatology,
NDORMS,
Oxford,
Anne
Simonsen
orcid.org/0000-0003-4711-7057
Basic
Oslo,
Reprogramming,
Oslo
Hospital
Montebello,
Alexandra
Stolz
orcid.org/0000-0002-3340-439X
Nektarios
Tavernarakis
orcid.org/0000-0002-5253-1466
Biotechnology,
Foundation
Technology-Hellas,
Heraklion,
Crete,
Greece
Sharon
Tooze
orcid.org/0000-0002-2182-3116
Francis
Crick
London,
Tamotsu
Yoshimori
orcid.org/0000-0001-9787-3788
Osaka
Suita,
Intracellular
Membrane
Dynamics,
Frontier
Integrated
Science
Division,
Open
Transdisciplinary
Initiatives
(OTRI),
Junying
Yuan
Interdisciplinary
on
Chemistry,
Shanghai
Organic
Shanghai,
Harvard
Boston,
Zhenyu
Yue
Neurology,
Friedman
Brain
Icahn
Mount
Sinai,
Qing
Zhong
orcid.org/0000-0001-6979-955X
Key
Differentiation
Apoptosis
Ministry
Education,
Pathophysiology,
Jiao
Tong
(SJTU-SM),
Lorenzo
Galluzzi
Corresponding
Author
[email
protected]
orcid.org/0000-0003-2257-8500
Dermatology,
Yale
Haven,
CT,
Pietrocola
orcid.org/0000-0002-2930-234X
Biosciences
Nutrition,
Huddinge,
mor
Nanoscale Horizons,
Год журнала:
2021,
Номер
6(5), С. 364 - 378
Опубликована: Янв. 1, 2021
The
nanoarchitectonics
concept
has
the
ability
to
bridge
nanoscale
science
and
visual
size
materials.
final
goal
of
approaches
is
creation
living-creature-like
functional
material
systems
from
simple
objects.
Mitochondrial
function
is
critically
dependent
on
the
folding
of
mitochondrial
inner
membrane
into
cristae;
indeed,
numerous
human
diseases
are
associated
with
aberrant
crista
morphologies.
With
MICOS
complex,
OPA1
and
F1
Fo
-ATP
synthase,
key
players
cristae
biogenesis
have
been
identified,
yet
their
interplay
poorly
understood.
Harnessing
super-resolution
light
3D
electron
microscopy,
we
dissect
roles
these
proteins
in
formation
mitochondria.
We
individually
disrupted
genes
all
seven
subunits
cells
re-expressed
Mic10
or
Mic60
respective
knockout
cell
line.
demonstrate
that
assembly
complex
triggers
remodeling
pre-existing
unstructured
de
novo
junctions
(CJs)
existing
cristae.
show
Mic60-subcomplex
sufficient
for
CJ
formation,
whereas
Mic10-subcomplex
controls
lamellar
biogenesis.
stabilizes
tubular
CJs
and,
along
fine-tunes
positioning
CJs.
propose
a
new
model
involving
coordinated
an
precursor
multiple
