Nucleic Acids Research,
Год журнала:
2022,
Номер
50(18), С. 10643 - 10664
Опубликована: Сен. 26, 2022
Asymmetric
subcellular
mRNA
localization
allows
spatial
regulation
of
gene
expression
and
functional
compartmentalization.
In
neurons,
specific
mRNAs
to
neurites
is
essential
for
cellular
functioning.
However,
it
largely
unknown
how
transcript
sorting
works
in
a
sequence-specific
manner.
Here,
we
combined
transcriptomics
massively
parallel
reporter
assays
tested
∼50
000
sequences
their
ability
localize
neurites.
Mapping
the
potential
>300
genes
revealed
two
ways
neurite
targeting
can
be
achieved:
focused
motifs
broadly
encoded
potential.
We
characterized
interplay
between
RNA
stability
identified
able
bias
towards
or
soma
as
well
trans-acting
factors
required
action.
Based
on
our
data,
devised
machine
learning
models
that
were
predict
behavior
novel
sequences.
Testing
this
predictor
native
sequencing
data
showed
good
agreement
predicted
observed
potential,
suggesting
rules
uncovered
by
MPRA
also
apply
full-length
transcripts.
Current Opinion in Genetics & Development,
Год журнала:
2025,
Номер
92, С. 102328 - 102328
Опубликована: Март 12, 2025
The
development
of
multicellular
organisms
relies
on
the
precise
coordination
molecular
events
across
multiple
spatial
and
temporal
scales.
Understanding
how
information
flows
from
interactions
to
cellular
processes
tissue
organization
during
is
crucial
for
explaining
remarkable
reproducibility
complex
organisms.
This
review
explores
chromatin-encoded
transduced
localized
transcriptional
global
gene
expression
patterns,
highlighting
challenge
bridging
these
We
discuss
recent
experimental
findings
theoretical
frameworks,
emphasizing
polymer
physics
as
a
tool
describing
relationship
between
chromatin
structure
dynamics
By
integrating
perspectives,
we
aim
clarify
regulation
coordinated
levels
biological
suggest
strategies
future
approaches.
Journal of Alzheimer s Disease,
Год журнала:
2021,
Номер
83(3), С. 977 - 1001
Опубликована: Авг. 17, 2021
Oxidative
stress
is
associated
with
the
progression
of
Alzheimer's
disease
(AD).
Reactive
oxygen
species
can
modify
lipids,
DNA,
RNA,
and
proteins
in
brain.
The
products
their
peroxidation
oxidation
are
readily
detectable
at
incipient
stages
disease.
Based
on
these
products,
various
biomarker-based
strategies
have
been
developed
to
identify
oxidative
levels
AD.
Known
stress-related
biomarkers
include
lipid
F2-isoprostanes,
as
well
malondialdehyde
4-hydroxynonenal
which
both
conjugate
specific
amino
acids
proteins,
DNA
or
RNA
8-hydroxy-2'-deoxyguanosine
(8-OHdG)
8-hydroxyguanosine
(8-OHG),
respectively.
inducible
enzyme
heme
oxygenase
type
1
(HO-1)
found
be
upregulated
response
events
AD
While
global
for
early-stage
AD,
they
generally
poorly
differentiate
from
other
neurodegenerative
disorders
that
also
coincide
stress.
Redox
proteomics
approaches
provided
specificity
stress-associated
pathology
by
identification
oxidatively
damaged
pathology-specific
proteins.
In
this
review,
we
discuss
potential
combined
diagnostic
value
reported
context
eight
mRNA
newly
identified
using
a
transcriptomics
approach.
We
review
genes
involvement
regulation
Further
research
warranted
establish
protein
functionalities
molecular
mechanisms
involved
determination
status
patients.
Wiley Interdisciplinary Reviews - RNA,
Год журнала:
2022,
Номер
13(6)
Опубликована: Фев. 14, 2022
Spatial
segregation
of
mRNAs
in
the
cytoplasm
cells
is
a
well-known
biological
phenomenon
that
widely
observed
diverse
species
spanning
different
kingdoms
life.
In
mammalian
cells,
localization
has
been
documented
and
studied
quite
extensively
highly
polarized
most
notably
neurons,
where
localized
function
to
direct
protein
production
at
sites
are
distant
from
soma.
Recent
studies
have
strikingly
revealed
large
proportion
cellular
transcriptome
exhibits
distributions
even
lack
an
obvious
need
for
long-range
transport,
such
as
fibroblasts
or
epithelial
cells.
This
review
focuses
on
emerging
concepts
regarding
functional
outcomes
mRNA
targeting
We
also
discuss
regulatory
mechanisms
controlling
these
events,
with
emphasis
role
cell
mechanics
organization
cytoskeleton.
article
categorized
under:
Translation
>
Regulation
RNA
Export
Localization
Localization.
Nucleic Acids Research,
Год журнала:
2022,
Номер
50(18), С. 10643 - 10664
Опубликована: Сен. 26, 2022
Asymmetric
subcellular
mRNA
localization
allows
spatial
regulation
of
gene
expression
and
functional
compartmentalization.
In
neurons,
specific
mRNAs
to
neurites
is
essential
for
cellular
functioning.
However,
it
largely
unknown
how
transcript
sorting
works
in
a
sequence-specific
manner.
Here,
we
combined
transcriptomics
massively
parallel
reporter
assays
tested
∼50
000
sequences
their
ability
localize
neurites.
Mapping
the
potential
>300
genes
revealed
two
ways
neurite
targeting
can
be
achieved:
focused
motifs
broadly
encoded
potential.
We
characterized
interplay
between
RNA
stability
identified
able
bias
towards
or
soma
as
well
trans-acting
factors
required
action.
Based
on
our
data,
devised
machine
learning
models
that
were
predict
behavior
novel
sequences.
Testing
this
predictor
native
sequencing
data
showed
good
agreement
predicted
observed
potential,
suggesting
rules
uncovered
by
MPRA
also
apply
full-length
transcripts.
