Neuron type-specific mRNA translation programs provide a gateway for memory consolidation.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Summary Long-term memory consolidation is a dynamic process that requires heterogeneous ensemble of neurons, each with highly specialized molecular environment. Considerable effort has been placed into understanding how the mechanisms in specific neuron types are modified memory, but these studies often undertaken hours or days after training, when already consolidated. Studies have shown protein synthesis elevated during early stages consolidation, there limited information as to it impacts neuronal function. We hypothesize mRNAs being translated could provide clues diverse neurons involved formation restructure their architecture support formation. Here, we generate landscape translatome three dorsal hippocampus first hour contextual consolidation. Our results show share common backbone readily mRNAs. However, excitatory undergo deep reconfiguration proteostatic control, whereas interneurons modify synaptic transmission. demonstrate translational control GADD34, which promotes translation initiation. Finally, differential expression by can be explained features hard coded mRNA, suggesting ubiquitous controlling activity-dependent translation. Altogether, our work uncovers previously unknown checkpoints and provides large, available resource for further investigations health disease.

Language: Английский

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A Review of Oxidative Stress Products and Related Genes in Early Alzheimer’s Disease

Journal of Alzheimer s Disease, Journal Year: 2021, Volume and Issue: 83(3), P. 977 - 1001

Published: Aug. 17, 2021

Oxidative stress is associated with the progression of Alzheimer's disease (AD). Reactive oxygen species can modify lipids, DNA, RNA, and proteins in brain. The products their peroxidation oxidation are readily detectable at incipient stages disease. Based on these products, various biomarker-based strategies have been developed to identify oxidative levels AD. Known stress-related biomarkers include lipid F2-isoprostanes, as well malondialdehyde 4-hydroxynonenal which both conjugate specific amino acids proteins, DNA or RNA 8-hydroxy-2'-deoxyguanosine (8-OHdG) 8-hydroxyguanosine (8-OHG), respectively. inducible enzyme heme oxygenase type 1 (HO-1) found be upregulated response events AD While global for early-stage AD, they generally poorly differentiate from other neurodegenerative disorders that also coincide stress. Redox proteomics approaches provided specificity stress-associated pathology by identification oxidatively damaged pathology-specific proteins. In this review, we discuss potential combined diagnostic value reported context eight mRNA newly identified using a transcriptomics approach. We review genes involvement regulation Further research warranted establish protein functionalities molecular mechanisms involved determination status patients.

Language: Английский

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Cotranslational Mechanisms of Protein Biogenesis and Complex Assembly in Eukaryotes

Annual Review of Biomedical Data Science, Journal Year: 2022, Volume and Issue: 5(1), P. 67 - 94

Published: April 26, 2022

The formation of protein complexes is crucial to most biological functions. cellular mechanisms governing complex biogenesis are not yet well understood, but some principles cotranslational and posttranslational assembly beginning emerge. In bacteria, this process favored by operons encoding subunits complexes. Eukaryotic cells do have polycistronic mRNAs, raising the question how they orchestrate encounter unassembled subunits. Here we review constraints eukaryotic co- folding assembly, including influence elongation rate on nascent chain targeting, folding, chaperone interactions. Recent evidence shows that mRNAs oligomeric assemblies can undergo localized translation form cytoplasmic condensates might facilitate Understanding interplay between mRNA proteostasis will be critical defining in vivo.

Language: Английский

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Developmental toxicity of TCBPA on the nervous and cardiovascular systems of zebrafish (Danio rerio): A combination of transcriptomic and metabolomics

Journal of Environmental Sciences, Journal Year: 2022, Volume and Issue: 127, P. 197 - 209

Published: April 30, 2022

Language: Английский

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Regulation and outcomes of localized RNA translation

Wiley Interdisciplinary Reviews - RNA, Journal Year: 2022, Volume and Issue: 13(6)

Published: Feb. 14, 2022

Spatial segregation of mRNAs in the cytoplasm cells is a well-known biological phenomenon that widely observed diverse species spanning different kingdoms life. In mammalian cells, localization has been documented and studied quite extensively highly polarized most notably neurons, where localized function to direct protein production at sites are distant from soma. Recent studies have strikingly revealed large proportion cellular transcriptome exhibits distributions even lack an obvious need for long-range transport, such as fibroblasts or epithelial cells. This review focuses on emerging concepts regarding functional outcomes mRNA targeting We also discuss regulatory mechanisms controlling these events, with emphasis role cell mechanics organization cytoskeleton. article categorized under: Translation > Regulation RNA Export Localization Localization.

Language: Английский

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