Biophysica,
Год журнала:
2024,
Номер
4(4), С. 604 - 618
Опубликована: Ноя. 28, 2024
Tissue
spheroids
are
self-organised
3D
cellular
aggregates
that
serve
as
a
versatile
platform
in
tissue
engineering.
While
numerous
high-throughput
methods
exist
to
characterise
the
function
of
spheroids,
equivalent
techniques
for
mechanical
characterisation
still
lacking.
In
this
review,
we
focus
on
fusion—
simple,
fast,
and
inexpensive
method
rheology
spheroids.
We
begin
by
discussing
implications
development
disease,
followed
detailed
explanation
how
phenomenon
arrested
coalescence
can
be
used
explore
Finally,
present
different
theoretical
models
that,
when
combined
with
experimental
data,
allow
us
extract
rheological
information.
Mechanical
force
initiates
sterile
inflammation,
a
process
implicated
in
diverse
physiological
and
pathological
processes.
The
timely
clearance
of
apoptotic
cells
by
macrophages
via
efferocytosis
is
crucial
for
the
proper
resolution
inflammation
averting
excessive
tissue
damage.
Despite
this,
specific
role
underlying
mechanisms
mechanical
on
macrophage
remain
obscure.
By
integrating
bioinformatics
metabolomics
analyses,
we
uncovered
how
disrupts
"arginine
metabolism─TCA
cycle─mitochondrial
function"
metabolic
cascade,
thereby
impairing
intensifying
inflammation.
Notably,
discovered
that
elevating
l-arginine
levels
can
ameliorate
these
crises
restoring
energy
metabolism.
Leveraging
this
insight,
engineered
microneedle
drug
delivery
system
loaded
with
nitric-oxide
driven
nanomotors
(MSN-LA@MNs)
targeted
l-arginine.
active
component,
MSN-LA,
exploits
heightened
expression
inducible
nitric
oxide
synthase
(iNOS)
force-loaded
tissues
as
chemoattractant,
harnessing
NO
generated
from
iNOS-catalyzed
autonomous
propulsion.
In
force-induced
rat
orthodontic
tooth
movement
(OTM)
model,
confirmed
MSN-LA@MNs
enhance
and,
under
iNOS
guidance,
dynamically
modulate
OTM,
thus
facilitating
OTM
process.
Collectively,
our
findings
elucidate
previously
unclear
mechanistic
links
between
force,
efferocytosis,
vantage
point,
offering
promising
strategy
modulating
force-related
biological
processes
such
OTM.
The
myocardial
wall
arises
from
a
single
layer
of
cardiomyocytes,
some
delaminate
to
create
trabeculae
while
others
remain
in
the
compact
layer.
However,
mechanisms
governing
cardiomyocyte
fate
decisions
unclear.
Using
single-cell
RNA
sequencing,
genetically
encoded
biosensors,
and
toto
live
imaging,
we
observe
intrinsic
variations
erbb2
expression
its
association
with
trabecular
fate.
Specifically,
promotes
PI3K
activity
recruits
Arp2/3
complex,
inducing
polarized
accumulation
actomyosin
network
drive
cell
delamination.
Subsequently,
lineage-committed
nascent
trigger
Notch
neighboring
cardiomyocytes
suppress
reduce
tension,
thereby
confining
them
Overall,
this
genetic
cellular
interplay
governs
determination
orchestrate
pattern
formation.
Materials Today Bio,
Год журнала:
2025,
Номер
31, С. 101645 - 101645
Опубликована: Март 8, 2025
Polyetheretherketone
(PEEK)
has
potential
to
repair
the
orbital
floor
bone
defects
following
craniofacial
trauma
and
surgery.
However,
inertness
of
material
impedes
soft
tissue
integration
implants,
leading
complications
such
as
implant
migration
infection.
Surface
patterning
modification
on
PEEK
can
promote
surface
hydrophily
enhance
better
integration,
but
it
is
difficult
obtain
uniform
controllable
nano-structure.
In
this
study,
hot-pressing
technology
was
used
produce
nanopores
with
a
diameter
200,
500,
800
nm.
Depending
craft,
surficial
mechanical
properties
be
regulated
assessed
by
finite
element
analysis.
Furthermore,
500
nm
interface
proliferation,
migration,
fibrosis
fibroblasts
achieved
optimal
effects
in
animal
implantation
experiments.
To
explore
mechanism
biological
responses,
transcriptomics
molecular
biology
experiments
revealed
that
Piezo1/TGF-β1
axis
played
critical
role
response
cells
stimulation
PEEK.
Our
study
established
novel
for
constructing
nanostructures
PEEK,
thereby
promoting
tissues
implants
improving
anchoring
effect.
Biomaterials Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Functional
tissue
regeneration
still
remains
a
challenge
in
some
tissues,
while
lack
of
comprehensive
understanding
about
regenerative
biology
as
well
roles
biomaterials
play
significantly
hinder
its
advances.
ACS Omega,
Год журнала:
2025,
Номер
10(19), С. 19675 - 19681
Опубликована: Май 6, 2025
Tissue
densification
is
a
fundamental
biological
process
involved
in
development,
regeneration,
and
disease,
significantly
influencing
tissue
mechanics
cellular
mechanical
microenvironments.
However,
the
effects
of
on
properties,
roles
key
cytoskeletal
components,
their
responses
to
external
stress
remain
poorly
understood.
In
this
study,
fibroblast-collagen
microtissues
were
cultured
polydimethylsiloxane
(PDMS)
microstring
scaffolds
with
different
constraints
generate
low-
high-densification
microtissues.
High-precision
indentation
force
sensor
analysis
demonstrated
that
increased
enhanced
microtissue
stiffness,
accelerated
relaxation,
elevated
energy
dissipation.
Pharmacological
disruption
cytoskeletons
revealed
critical
role
F-actin
regulating
stiffness
viscoelastic
resistance
densification-dependent
manner:
microtissues,
had
greater
impact
dissipated
energy,
but
reduced
effect
relaxation.
Furthermore,
under
50%
strain
indentation,
exhibited
irreversible
deformation
injury.
Injured
regions
showed
shorter
relaxation
time
constants,
higher
dissipation,
indicating
structural
damage.
These
results
enhance
our
understanding
mechanobiology
by
elucidating
interplay
between
densification,
mechanics,
injury
responses,
providing
valuable
insights
for
optimizing
microenvironments
improving
compatibility
biomaterials.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 11, 2025
Abstract
In
ever‐increasing
numbers,
patients
are
treated
with
biodegradable
magnesium
implants.
While
gas
bubbles
frequently
arise
in
soft
tissue
overlying
implants,
their
biological
implications
remain
uncertain.
This
study
investigates
how
bubble
accumulation
and
evolution
across
various
lengths
time
scales
influence
adjacent
cell
behavior
rats.
Bubbles
accumulate
tissues
around
during
initial
postimplantation
days,
then
fully
resorb.
Alterations
geometry
coincide
of
cells,
many
macrophage
phenotypes,
increased
expression
the
mechanosensitive
ion‐channel
Piezo1.
Using
spatially
resolved
transcriptomics,
strong
proinflammatory
pathway
activation
is
revealed
near
marked
proliferative
marker
secreted
phosphoprotein
1
(
Spp1
).
Spatial
transcriptomics
also
reveals
enrichment
cytoskeletal
rearrangement
genes,
demonstrating
that
cells
respond
to
mechanical
cues
from
bubbles.
Notably,
both
bubble–implant
distance
strongly
cellular
response.
Over
time,
as
located
farther
implant,
regenerative
processes
decline,
inflammation
predominates.
These
findings
suggest
implant
degradation
create
an
intricate
local
response
influencing
healing
through
inflammatory
pathways.
underscores
need
for
implants
controlled
release
meticulous
monitoring
patients.
Biomolecules,
Год журнала:
2025,
Номер
15(6), С. 765 - 765
Опубликована: Май 26, 2025
Muscle
stem
cells
(MuSCs)
are
the
key
to
muscle
regeneration.
The
activation
and
maintenance
of
MuSCs
require
precise
regulation
their
microenvironments.
Myofibers
other
including
endothelial
cells,
fibroblasts,
immune
cell
populations
constitute
components
MuSC
niche.
communication
between
these
play
an
essential
role
in
repair.
Furthermore,
physical
chemical
stimulations
around
also
affect
behaviors
MuSCs.
Extracellular
matrix
(ECM)
factors
stored
it
generate
a
repair-promoting
niche
for
efficient
Understanding
mechanism
is
basis
clinically
optimizing
In
this
review,
we
discuss
recent
findings
about
microenvironments
functions
regeneration,
which
would
shed
light
on
new
targets
strategies
injury
treatment.
