Surface Modification of Biodegradable Polymer Enhances Liver Organoid Attachment and Integration Forming a Cholangiocyte Monolayer DOI
Junzhi Li,

Jing Chu,

Zhenlin Chen

et al.

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Cell adhesion is a fundamental necessity for anchorage-dependent cells to thrive in the matrix. This process serves as initial stage sequence of cellular activities, which includes cell diffusion, migration, proliferation, and differentiation. study introduces novel surface modification designed engineered enhance attachment cholangiocyte organoids facilitate spreading cholangiocytes on polymer surface. Our findings revealed that recruiting collagen layer polydopamine (PDA) nanoparticle coating poly(lactide-co-trimethylene carbonate) (PLATMC) plays key role attracting liver enhancing migration. finding has promising results generating two-dimensional (2D) monolayer through organoid subsequent integration. The innovative approach combines with culture technique, offering significant potential advancing bile duct regenerative medicine developing more complex three-dimensional (3D) tissues vitro.

Language: Английский

Key wound healing genes as diagnostic biomarkers and therapeutic targets in uterine corpus endometrial carcinoma: an integrated in silico and in vitro study DOI Creative Commons
Feng Jiang,

Sajjad Ahmad,

Sumaira Kanwal

et al.

Hereditas, Journal Year: 2025, Volume and Issue: 162(1)

Published: Jan. 21, 2025

Abstract Background Uterine Corpus Endometrial Carcinoma (UCEC) is a prevalent gynecologic malignancy with complex molecular underpinnings. This study identifies key woundhealing genes involved in UCEC and elucidates their roles through comprehensive analysis. Methods In silico vitro experiments. Results Seventy wound healing-associated were extracted from the Gene Ontology (GO) database, protein-protein interaction (PPI) network was constructed using STRING database. CytoHubba analysis Cytoscape identified six pivotal hub genes: CD44, FGF2, FGF10, KDM6A, FN1, MMP2. These exhibited significantly lower expression cell lines compared to normal controls, as confirmed by RT-qPCR. Receiver Operating Characteristic (ROC) demonstrated potential diagnostic biomarkers, Area Under Curve (AUC) values ranging 0.94 1.00. Validation TCGA datasets revealed consistent downregulation of these samples, corroborated immunohistochemical staining. Promoter methylation showed higher levels UCEC, correlating decreased mRNA poor survival outcomes. Genetic alteration indicated frequent mutations FN1 although did not affect survival. Functional CancerSEA database highlighted involvement critical cancer-related processes, including angiogenesis, apoptosis, metastasis. Immune correlation studies significant associations immune inhibitor distinct patterns across subtypes. Overexpression that CD44 MMP2 reduce proliferative ability while enhancing migration healing. Conclusion Collectively, findings underscore crucial pathogenesis, highlighting biomarkers therapeutic targets this malignancy.

Language: Английский

Citations

2
Cancer stem cells and niches: challenges in immunotherapy resistance DOI Creative Commons
Yonglong Pan,

Chaoyi Yuan,

Chenglong Zeng

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: Feb. 25, 2025

Cancer stem cells (CSCs) are central to tumor progression, metastasis, immune evasion, and therapeutic resistance. Characterized by remarkable self-renewal adaptability, CSCs can transition dynamically between stem-like differentiated states in response external stimuli, a process termed "CSC plasticity." This adaptability underpins their resilience therapies, including checkpoint inhibitors adoptive cell therapies (ACT). Beyond intrinsic properties, reside specialized microenvironment—the CSC niche—which provides immune-privileged protection, sustains stemness, fosters suppression. review highlights the critical role of niche driving immunotherapy resistance, emphasizing need for integrative approaches overcome these challenges.

Language: Английский

Citations

2
Comparative transcriptome analysis identified genes involved in testicular development in Takifugu rubripes DOI

Jinfeng Chen,

Mingtao Hu,

Meiyuan Li

et al.

Fish Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 51(1)

Published: Jan. 6, 2025

Language: Английский

Citations

1
Fibrinogen: a new player and target on the formation of pre-metastatic niche in tumor metastasis DOI
Yuxin Zhang, Zelin Li,

Jiamao Zhang

et al.

Critical Reviews in Oncology/Hematology, Journal Year: 2025, Volume and Issue: 207, P. 104625 - 104625

Published: Jan. 17, 2025

Language: Английский

Citations

1
Tailoring Extracellular Matrix Niches: Impact of Glycosaminoglycan Content on Multiple Differentiation of Human Mesenchymal Stem Cells DOI Creative Commons
Xingxing Yang, Meeta Mistry, Abigail Dee Chen

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: unknown, P. 123130 - 123130

Published: Jan. 1, 2025

Language: Английский

Citations

1
Transcriptome analyses suggest distinct master sex determination genes in closely related blue catfish and channel catfish DOI
Tong Chen,

Dongya Gao,

Baofeng Su

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: 603, P. 742364 - 742364

Published: March 6, 2025

Language: Английский

Citations

1
Structural glycobiology – from enzymes to organelles DOI Creative Commons
Courtney J. Mycroft‐West, Miron Leanca, Liang Ping Wu

et al.

Biochemical Society Transactions, Journal Year: 2025, Volume and Issue: 53(1)

Published: Jan. 31, 2025

Biological carbohydrate polymers represent some of the most complex molecules in life, enabling their participation a huge range physiological functions. The complexity biological carbohydrates arises from an extensive enzymatic repertoire involved construction, deconstruction and modification. Over past decades, structural studies processing enzymes have driven major insights into mechanisms, supporting associated applications across medicine biotechnology. Despite these successes, our understanding how multienzyme networks function to create polysaccharides is still limited. Emerging techniques such as super-resolution microscopy cryo-electron tomography are now investigation native systems at near molecular resolutions. Here, we review classical vitro processing, alongside recent situ glycosylation-related processes. While considerable technical challenges remain, integration mechanisms with true context promises transform regulation, shining light upon processes driving functional essential biomolecules.

Language: Английский

Citations

0
EDA Fibronectin Microarchitecture and YAP Translocation during Wound Closure DOI

Jennifer Patten,

Patrick D. Halligan,

Ghazal Bashiri

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Fibronectin (Fn) is an extracellular matrix glycoprotein with mechanosensitive structure–function. Extra domain A (EDA) Fn, a Fn isoform, not present in adult tissue but required for repair. Curiously, EDA linked to both regenerative and fibrotic Given that mechanoregulates cell behavior, organization during wound closure might play role mediating these differing responses. One mechanism by which cells sense respond their microenvironment activating transcriptional coactivator, yes-associated protein (YAP). Interestingly, YAP activity only similarly Therefore, this study aims evaluate how, normal closure, modulate translocation culturing human dermal fibroblasts on polydimethylsiloxane substrates mimicking (soft: 18 kPa) (stiff: 146 wounded skin. On stiffer wounds, assembled aligned comprising thinner fibers, suggesting increased microenvironmental tension. To if binding the of was essential overall organization, were treated Irigenin, inhibits within Fn. Blocking adhesion led randomly organized matrices thicker reduced tension even closure. whether signaling plays CA3, suppresses dose-dependent manner. Treatment CA3 also potential connected reducing Next, assessed impact organization. migrating softer wounds activity, substrates, they decreased activity. When Irigenin or These results suggest there may be disrupted between could restored when reestablishing instead drive

Language: Английский

Citations

0
Fibroblast-derived versican exacerbates periodontitis progression by regulating macrophage migration and inflammatory cytokine secretion DOI
Yuting Yang, Li Li, Fang Dai

et al.

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111755 - 111755

Published: March 1, 2025

Language: Английский

Citations

0
Molecular dynamics of the matrisome across sea anemone life history DOI Open Access
Bruno Gideon Bergheim, Alison G. Cole, Mandy Rettel

et al.

Published: April 2, 2025

The evolutionary expansion of extracellular matrix (ECM) molecules has been crucial for the establishment cell adhesion and transition from unicellular to multicellular life. Members pre-bilaterian phylum cnidaria offer an exceptionally rich perspective into metazoan core adhesome its original function in developmental morphogenetic processes. Here, we present ensemble ECM proteins associated factors starlet sea anemone Nematostella vectensis based on silico prediction quantitative proteomic analysis decellularized mesoglea different life stages. integration matrisome with single transcriptome atlases reveals that Nematostella’s complex is predominantly produced by gastrodermal cells, confirming homology cnidarian inner layer bilaterian mesoderm. larva polyp characterized upregulation metalloproteases basement membrane components including all members unusually diversified SVEP1/Polydom family, suggesting massive epithelial remodeling. enrichment Wnt/PCP pathway during this process further indicates directed rearrangements as a key contributor polyp’s morphogenesis. Mesoglea maturation adult polyps involves wound response similar molecular patterns growth regeneration. Our study identifies conserved matrisomal networks coordinate transitions history.

Language: Английский

Citations

0