bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 31, 2024
Abstract
Disruption
of
cochlear
architecture
and
development
can
lead
to
malfunction,
resulting
in
hearing
defects.
However,
the
spatial
molecular
profiles
critical
for
function
remain
poorly
understood
due
structural
complexity
cochlea.
In
this
study,
we
performed
comprehensive
spatiotemporal
transcriptomic
analyses
on
developing
adult
cochlea,
identifying
numerous
genes
with
gradient
expression
patterns
hair
cells
(HCs)
spiral
ganglion
neurons
(SGNs)
across
apical-to-basal
axis.
The
gene
Myo7a
,
a
well-known
HC
marker,
established
decreasing
outer
(OHCs)
from
apical
basal
regions
adulthood.
contrast,
other
class
exhibited
an
opposing
pattern
along
axis
adulthood,
such
as
Calb2
HCs
Nefh
SGNs,
which
potentially
corresponds
tuning
properties
sensing
frequency
sounds.
Importantly,
our
provided
compelling
evidences
existence
distinct
subtypes
OHCs.
revealed
regional
heterogeneity
cell
communication
intensity
between
weakest
at
apex,
aligning
region
detection
low-frequency
sound.
This
study
systematically
characterized
fine
organizations
HCs,
their
regionalized
cell-communications
within
offering
insights
into
morphological
foundations
underlying
tonotopic
organization
discrimination.
Gut,
Год журнала:
2025,
Номер
unknown, С. gutjnl - 334165
Опубликована: Фев. 4, 2025
Inflammatory
bowel
diseases
(IBDs),
which
include
ulcerative
colitis
(UC)
and
Crohn’s
disease
(CD),
are
chronic
conditions
characterised
by
inflammation
of
the
intestinal
tract.
Alterations
in
virtually
all
cell
types,
including
immune,
epithelial
stromal
cells,
have
been
described
these
diseases.
The
study
IBD
has
historically
relied
on
bulk
transcriptomics,
but
this
method
averages
signals
across
diverse
limiting
insights.
Single-cell
omic
technologies
overcome
intrinsic
limitations
analysis
reveal
complexity
multicellular
tissues
at
a
cell-by-cell
resolution.
Within
healthy
inflamed
tissues,
single-cell
omics,
particularly
RNA
sequencing,
contributed
to
uncovering
novel
types
functions
linked
activity
or
development
complications.
Collectively,
results
help
identify
therapeutic
targets
difficult-to-treat
complications
such
as
fibrostenosis,
creeping
fat
accumulation,
perianal
fistulae
pouch.
More
recently,
omics
gradually
adopted
studies
understand
responses,
mechanisms
drug
failure
potentially
develop
predictors
with
clinical
utility.
Although
early
days,
lay
groundwork
for
implementation
practice
new
diagnostics,
monitoring
prediction
prognosis.
With
review,
we
aim
provide
comprehensive
survey
that
applied
UC
CD,
offer
our
perspective
main
findings
contribute.
Finally,
discuss
potential
benefits
integration
into
could
offer.
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 26, 2025
Triple-negative
breast
cancer
(TNBC)
is
a
highly
malignant
tumor
in
women,
characterized
by
high
morbidity,
mortality,
and
recurrence
rates.
Although
surgical
treatment,
radiotherapy,
chemotherapy
are
the
mainstays
of
current
treatment
methods,
heterogeneity
TNBC
results
unsatisfactory
outcomes
with
low
5-year
survival
Rapid
advancements
omics
technology
have
propelled
understanding
molecular
biology.
The
emergence
single-cell
RNA
sequencing
(scRNA-seq)
spatial
transcriptomics
(ST)
has
significantly
enhanced
knowledge
distribution,
functionality,
intercellular
interactions
various
cell
types
within
microenvironment,
including
cells,
T
B
macrophages,
fibroblasts.
present
study
provides
an
overview
technical
characteristics
scRNA-seq
ST,
highlighting
their
applications
exploring
heterogeneity,
distribution
patterns,
interactions.
This
review
aims
to
enhance
comprehension
at
cellular
level
for
development
effective
therapeutic
targets.
