Cell Death and Differentiation,
Год журнала:
2021,
Номер
28(10), С. 2957 - 2969
Опубликована: Июль 20, 2021
Abstract
SidE
family
of
Legionella
effectors
catalyze
non-canonical
phosphoribosyl-linked
ubiquitination
(PR-ubiquitination)
host
proteins
during
bacterial
infection.
SdeA
localizes
predominantly
to
ER
and
partially
the
Golgi
apparatus,
mediates
serine
multiple
proteins.
Here
we
show
that
causes
disruption
integrity
due
its
ubiquitin
ligase
activity.
The
linking
GRASP55
GRASP65
are
PR-ubiquitinated
on
residues,
thus
preventing
their
ability
cluster
form
oligomeric
structures.
In
addition,
found
functional
consequence
is
not
linked
recruitment
membranes
growing
-containing
vacuoles.
Instead,
it
affects
secretory
pathway.
Taken
together,
our
study
sheds
light
manipulation
strategy
by
which
hijacks
pathway
promotes
Grazing
of
amoebae
on
microorganisms
represents
one
the
oldest
predator-prey
dynamic
relationships
in
nature.
It
a
genetic
"melting
pot"
for
an
ancient
and
continuous
multi-directional
inter-
intra-kingdom
horizontal
gene
transfer
between
its
preys,
intracellular
microbial
residents,
endosymbionts,
giant
viruses,
which
has
shaped
evolution,
selection,
adaptation
microbes
that
evade
degradation
by
predatory
amoeba.
Unicellular
phagocytic
are
thought
to
be
ancestors
macrophages
with
highly
conserved
eukaryotic
processes.
Selection
evolution
within
amoeba
through
their
target
processes
have
facilitated
expansion
host
range
mammals,
causing
various
infectious
diseases.
Pseudokinases
are
considered
to
be
the
inactive
counterparts
of
conventional
protein
kinases
and
comprise
approximately
10%
human
mouse
kinomes.
Here,
we
report
crystal
structure
Legionella
pneumophila
effector
protein,
SidJ,
in
complex
with
eukaryotic
Ca2+-binding
regulator,
calmodulin
(CaM).
The
reveals
that
SidJ
contains
a
kinase-like
fold
domain,
which
retains
majority
characteristic
kinase
catalytic
motifs.
However,
fails
demonstrate
activity.
Instead,
mass
spectrometry
vitro
biochemical
analyses
modifies
another
SdeA,
an
unconventional
phosphoribosyl
ubiquitin
ligase,
by
adding
glutamate
molecules
specific
residue
SdeA
CaM-dependent
manner.
Furthermore,
show
SidJ-mediated
polyglutamylation
suppresses
ADP-ribosylation
Our
work
further
implies
some
pseudokinases
may
possess
ATP-dependent
activities
other
than
phosphorylation.
Computational and Structural Biotechnology Journal,
Год журнала:
2021,
Номер
19, С. 2366 - 2383
Опубликована: Янв. 1, 2021
ADP-ribosylation
is
an
ancient
posttranslational
modification
present
in
all
kingdoms
of
life.
The
system
likely
originated
bacteria
where
it
functions
inter-
and
intra-species
conflict,
stress
response
pathogenicity.
It
was
repeatedly
adopted
via
lateral
transfer
by
eukaryotes,
including
humans,
has
a
pivotal
role
epigenetics,
DNA-damage
repair,
apoptosis,
other
crucial
pathways
the
immune
to
pathogenic
viruses.
In
words,
same
ammunition
used
pathogens
adapted
eukaryotes
fight
back.
While
we
know
quite
lot
about
eukaryotic
system,
expanding
rather
patchy
knowledge
on
bacterial
viral
would
give
us
not
only
better
understanding
as
whole
but
fighting
advantage
this
constant
arms
race.
By
writing
review
hope
put
into
focus
available
information
perspective
how
works
can
be
exploited
search
for
therapeutic
targets
future.
relevance
subject
especially
highlighted
current
situation
being
amid
world
pandemic
caused
virus
harbouring
dependent
representative
such
system.
To
prevail
in
the
interaction
with
eukaryotic
hosts,
many
bacterial
pathogens
use
protein
secretion
systems
to
release
virulence
factors
at
host–pathogen
interface
and/or
deliver
them
directly
into
host
cells.
An
outstanding
example
of
complexity
and
sophistication
diversity
their
substrates,
effectors,
is
Defective
organelle
trafficking/Intracellular
multiplication
(Dot/Icm)
Type
IVB
system
(T4BSS)
Legionella
pneumophila
related
species.
species
are
facultative
intracellular
environmental
protozoa
opportunistic
human
respiratory
pathogens.
The
Dot/Icm
T4BSS
translocates
an
exceptionally
large
number
more
than
300
per
L.
strain,
essential
for
evasion
phagolysosomal
degradation
exploitation
macrophages
as
replicative
niches.
Recent
technological
advancements
imaging
complexes
have
provided
new
insight
architecture
allowed
us
propose
models
transport
mechanism.
At
same
time,
significant
progress
has
been
made
assigning
functions
about
a
third
discovering
unprecedented
enzymatic
activities
concepts
subversion.
In
this
review,
we
describe
current
knowledge
workings
machinery
provide
overview
to-date
characterized
effectors
Biochemical Society Transactions,
Год журнала:
2019,
Номер
47(6), С. 1857 - 1866
Опубликована: Дек. 17, 2019
Protein
ubiquitination
is
a
posttranslational
modification
that
regulates
many
aspects
of
cellular
life,
including
proteostasis,
vesicular
trafficking,
DNA
repair
and
NF-κB
activation.
By
directly
targeting
intracellular
bacteria
or
bacteria-containing
vacuoles
to
the
lysosome,
also
an
important
component
cell-autonomous
immunity.
Not
surprisingly,
several
pathogenic
encode
deubiquitinases
(DUBs)
use
them
as
secreted
effectors
prevent
bacterial
components.
A
systematic
overview
known
DUBs,
their
cleavage
specificities
biological
roles,
suggests
multiple
independent
acquisition
events
from
host-encoded
DUBs
other
proteases.
The
widely
used
classification
into
seven
well-defined
families
should
only
be
applied
eukaryotic
since
do
not
follow
this
classification.
Biochemical Journal,
Год журнала:
2022,
Номер
479(4), С. 463 - 477
Опубликована: Фев. 17, 2022
ADP-ribosylation
has
primarily
been
known
as
post-translational
modification
of
proteins.
As
signalling
strategy
conserved
in
all
domains
life,
it
modulates
substrate
activity,
localisation,
stability
or
interactions,
thereby
regulating
a
variety
cellular
processes
and
microbial
pathogenicity.
Yet
over
the
last
years,
there
is
increasing
evidence
non-canonical
forms
that
are
catalysed
by
certain
members
ADP-ribosyltransferase
family
go
beyond
traditional
protein
signalling.
New
macromolecular
targets
such
nucleic
acids
new
ADP-ribose
derivatives
have
established,
notably
extending
repertoire
Based
on
physiological
relevance
so
far,
deserves
its
recognition
next
to
which
we
therefore
review
following.
