Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infection DOI Creative Commons
Yaobin Liu, Rukmini Mukherjee,

Florian Bonn

и другие.

Cell Death and Differentiation, Год журнала: 2021, Номер 28(10), С. 2957 - 2969

Опубликована: Июль 20, 2021

Abstract SidE family of Legionella effectors catalyze non-canonical phosphoribosyl-linked ubiquitination (PR-ubiquitination) host proteins during bacterial infection. SdeA localizes predominantly to ER and partially the Golgi apparatus, mediates serine multiple proteins. Here we show that causes disruption integrity due its ubiquitin ligase activity. The linking GRASP55 GRASP65 are PR-ubiquitinated on residues, thus preventing their ability cluster form oligomeric structures. In addition, found functional consequence is not linked recruitment membranes growing -containing vacuoles. Instead, it affects secretory pathway. Taken together, our study sheds light manipulation strategy by which hijacks pathway promotes

Язык: Английский

Amoebae as training grounds for microbial pathogens DOI Creative Commons

Christopher T. D. Price,

Hannah E. Hanford,

Tasneem Al‐Quadan

и другие.

mBio, Год журнала: 2024, Номер 15(8)

Опубликована: Июль 8, 2024

Grazing of amoebae on microorganisms represents one the oldest predator-prey dynamic relationships in nature. It a genetic "melting pot" for an ancient and continuous multi-directional inter- intra-kingdom horizontal gene transfer between its preys, intracellular microbial residents, endosymbionts, giant viruses, which has shaped evolution, selection, adaptation microbes that evade degradation by predatory amoeba. Unicellular phagocytic are thought to be ancestors macrophages with highly conserved eukaryotic processes. Selection evolution within amoeba through their target processes have facilitated expansion host range mammals, causing various infectious diseases.

Язык: Английский

Процитировано

12

Structural basis of ubiquitin modification by the Legionella effector SdeA DOI
Yanan Dong, Yajuan Mu, Yongchao Xie

и другие.

Nature, Год журнала: 2018, Номер 557(7707), С. 674 - 678

Опубликована: Май 1, 2018

Язык: Английский

Процитировано

81

Protein polyglutamylation catalyzed by the bacterial calmodulin-dependent pseudokinase SidJ DOI Creative Commons
Alan Sulpizio, Marena E. Minelli, Min Wan

и другие.

eLife, Год журнала: 2019, Номер 8

Опубликована: Ноя. 4, 2019

Pseudokinases are considered to be the inactive counterparts of conventional protein kinases and comprise approximately 10% human mouse kinomes. Here, we report crystal structure Legionella pneumophila effector protein, SidJ, in complex with eukaryotic Ca2+-binding regulator, calmodulin (CaM). The reveals that SidJ contains a kinase-like fold domain, which retains majority characteristic kinase catalytic motifs. However, fails demonstrate activity. Instead, mass spectrometry vitro biochemical analyses modifies another SdeA, an unconventional phosphoribosyl ubiquitin ligase, by adding glutamate molecules specific residue SdeA CaM-dependent manner. Furthermore, show SidJ-mediated polyglutamylation suppresses ADP-ribosylation Our work further implies some pseudokinases may possess ATP-dependent activities other than phosphorylation.

Язык: Английский

Процитировано

67

ADP-ribosylation systems in bacteria and viruses DOI Creative Commons
Petra Mikolčević, Andrea Hloušek-Kasun, Ivan Ahel

и другие.

Computational and Structural Biotechnology Journal, Год журнала: 2021, Номер 19, С. 2366 - 2383

Опубликована: Янв. 1, 2021

ADP-ribosylation is an ancient posttranslational modification present in all kingdoms of life. The system likely originated bacteria where it functions inter- and intra-species conflict, stress response pathogenicity. It was repeatedly adopted via lateral transfer by eukaryotes, including humans, has a pivotal role epigenetics, DNA-damage repair, apoptosis, other crucial pathways the immune to pathogenic viruses. In words, same ammunition used pathogens adapted eukaryotes fight back. While we know quite lot about eukaryotic system, expanding rather patchy knowledge on bacterial viral would give us not only better understanding as whole but fighting advantage this constant arms race. By writing review hope put into focus available information perspective how works can be exploited search for therapeutic targets future. relevance subject especially highlighted current situation being amid world pandemic caused virus harbouring dependent representative such system.

Язык: Английский

Процитировано

48

To Ub or not to Ub: a regulatory question in TGF-β signaling DOI
Jinquan Liu, Jianping Jin,

Tingbo Liang

и другие.

Trends in Biochemical Sciences, Год журнала: 2022, Номер 47(12), С. 1059 - 1072

Опубликована: Июль 6, 2022

Язык: Английский

Процитировано

37

The Legionella pneumophila Dot/Icm type IV secretion system and its effectors DOI
Daniel C. Lockwood, Himani Amin, Tiago R. D. Costa

и другие.

Microbiology, Год журнала: 2022, Номер 168(5)

Опубликована: Май 23, 2022

To prevail in the interaction with eukaryotic hosts, many bacterial pathogens use protein secretion systems to release virulence factors at host–pathogen interface and/or deliver them directly into host cells. An outstanding example of complexity and sophistication diversity their substrates, effectors, is Defective organelle trafficking/Intracellular multiplication (Dot/Icm) Type IVB system (T4BSS) Legionella pneumophila related species. species are facultative intracellular environmental protozoa opportunistic human respiratory pathogens. The Dot/Icm T4BSS translocates an exceptionally large number more than 300 per L. strain, essential for evasion phagolysosomal degradation exploitation macrophages as replicative niches. Recent technological advancements imaging complexes have provided new insight architecture allowed us propose models transport mechanism. At same time, significant progress has been made assigning functions about a third discovering unprecedented enzymatic activities concepts subversion. In this review, we describe current knowledge workings machinery provide overview to-date characterized effectors

Язык: Английский

Процитировано

34

Ubiquitination of non-protein substrates DOI Creative Commons
Jun-Ichi Sakamaki, Noboru Mizushima

Trends in Cell Biology, Год журнала: 2023, Номер 33(11), С. 991 - 1003

Опубликована: Апрель 27, 2023

Язык: Английский

Процитировано

20

Ubiquitin‐targeted bacterial effectors: rule breakers of the ubiquitin system DOI
Cameron G. Roberts, Tyler G. Franklin, Jonathan N. Pruneda

и другие.

The EMBO Journal, Год журнала: 2023, Номер 42(18)

Опубликована: Авг. 9, 2023

Язык: Английский

Процитировано

20

Bacterial DUBs: deubiquitination beyond the seven classes DOI
Thomas Hermanns, Kay Hofmann

Biochemical Society Transactions, Год журнала: 2019, Номер 47(6), С. 1857 - 1866

Опубликована: Дек. 17, 2019

Protein ubiquitination is a posttranslational modification that regulates many aspects of cellular life, including proteostasis, vesicular trafficking, DNA repair and NF-κB activation. By directly targeting intracellular bacteria or bacteria-containing vacuoles to the lysosome, also an important component cell-autonomous immunity. Not surprisingly, several pathogenic encode deubiquitinases (DUBs) use them as secreted effectors prevent bacterial components. A systematic overview known DUBs, their cleavage specificities biological roles, suggests multiple independent acquisition events from host-encoded DUBs other proteases. The widely used classification into seven well-defined families should only be applied eukaryotic since do not follow this classification.

Язык: Английский

Процитировано

45

Beyond protein modification: the rise of non-canonical ADP-ribosylation DOI Creative Commons
M. Schuller, Ivan Ahel

Biochemical Journal, Год журнала: 2022, Номер 479(4), С. 463 - 477

Опубликована: Фев. 17, 2022

ADP-ribosylation has primarily been known as post-translational modification of proteins. As signalling strategy conserved in all domains life, it modulates substrate activity, localisation, stability or interactions, thereby regulating a variety cellular processes and microbial pathogenicity. Yet over the last years, there is increasing evidence non-canonical forms that are catalysed by certain members ADP-ribosyltransferase family go beyond traditional protein signalling. New macromolecular targets such nucleic acids new ADP-ribose derivatives have established, notably extending repertoire Based on physiological relevance so far, deserves its recognition next to which we therefore review following.

Язык: Английский

Процитировано

27