Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infection DOI Creative Commons
Yaobin Liu, Rukmini Mukherjee,

Florian Bonn

et al.

Cell Death and Differentiation, Journal Year: 2021, Volume and Issue: 28(10), P. 2957 - 2969

Published: July 20, 2021

Abstract SidE family of Legionella effectors catalyze non-canonical phosphoribosyl-linked ubiquitination (PR-ubiquitination) host proteins during bacterial infection. SdeA localizes predominantly to ER and partially the Golgi apparatus, mediates serine multiple proteins. Here we show that causes disruption integrity due its ubiquitin ligase activity. The linking GRASP55 GRASP65 are PR-ubiquitinated on residues, thus preventing their ability cluster form oligomeric structures. In addition, found functional consequence is not linked recruitment membranes growing -containing vacuoles. Instead, it affects secretory pathway. Taken together, our study sheds light manipulation strategy by which hijacks pathway promotes

Language: Английский

Amoebae as training grounds for microbial pathogens DOI Creative Commons

Christopher T. D. Price,

Hannah E. Hanford,

Tasneem Al‐Quadan

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(8)

Published: July 8, 2024

Grazing of amoebae on microorganisms represents one the oldest predator-prey dynamic relationships in nature. It a genetic "melting pot" for an ancient and continuous multi-directional inter- intra-kingdom horizontal gene transfer between its preys, intracellular microbial residents, endosymbionts, giant viruses, which has shaped evolution, selection, adaptation microbes that evade degradation by predatory amoeba. Unicellular phagocytic are thought to be ancestors macrophages with highly conserved eukaryotic processes. Selection evolution within amoeba through their target processes have facilitated expansion host range mammals, causing various infectious diseases.

Language: Английский

Citations

12
Structural basis of ubiquitin modification by the Legionella effector SdeA DOI
Yanan Dong, Yajuan Mu, Yongchao Xie

et al.

Nature, Journal Year: 2018, Volume and Issue: 557(7707), P. 674 - 678

Published: May 1, 2018

Language: Английский

Citations

81
Protein polyglutamylation catalyzed by the bacterial calmodulin-dependent pseudokinase SidJ DOI Creative Commons
Alan Sulpizio, Marena E. Minelli, Min Wan

et al.

eLife, Journal Year: 2019, Volume and Issue: 8

Published: Nov. 4, 2019

Pseudokinases are considered to be the inactive counterparts of conventional protein kinases and comprise approximately 10% human mouse kinomes. Here, we report crystal structure Legionella pneumophila effector protein, SidJ, in complex with eukaryotic Ca2+-binding regulator, calmodulin (CaM). The reveals that SidJ contains a kinase-like fold domain, which retains majority characteristic kinase catalytic motifs. However, fails demonstrate activity. Instead, mass spectrometry vitro biochemical analyses modifies another SdeA, an unconventional phosphoribosyl ubiquitin ligase, by adding glutamate molecules specific residue SdeA CaM-dependent manner. Furthermore, show SidJ-mediated polyglutamylation suppresses ADP-ribosylation Our work further implies some pseudokinases may possess ATP-dependent activities other than phosphorylation.

Language: Английский

Citations

67
ADP-ribosylation systems in bacteria and viruses DOI Creative Commons
Petra Mikolčević, Andrea Hloušek-Kasun, Ivan Ahel

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2021, Volume and Issue: 19, P. 2366 - 2383

Published: Jan. 1, 2021

ADP-ribosylation is an ancient posttranslational modification present in all kingdoms of life. The system likely originated bacteria where it functions inter- and intra-species conflict, stress response pathogenicity. It was repeatedly adopted via lateral transfer by eukaryotes, including humans, has a pivotal role epigenetics, DNA-damage repair, apoptosis, other crucial pathways the immune to pathogenic viruses. In words, same ammunition used pathogens adapted eukaryotes fight back. While we know quite lot about eukaryotic system, expanding rather patchy knowledge on bacterial viral would give us not only better understanding as whole but fighting advantage this constant arms race. By writing review hope put into focus available information perspective how works can be exploited search for therapeutic targets future. relevance subject especially highlighted current situation being amid world pandemic caused virus harbouring dependent representative such system.

Language: Английский

Citations

48
To Ub or not to Ub: a regulatory question in TGF-β signaling DOI
Jinquan Liu, Jianping Jin,

Tingbo Liang

et al.

Trends in Biochemical Sciences, Journal Year: 2022, Volume and Issue: 47(12), P. 1059 - 1072

Published: July 6, 2022

Language: Английский

Citations

37
The Legionella pneumophila Dot/Icm type IV secretion system and its effectors DOI
Daniel C. Lockwood, Himani Amin, Tiago R. D. Costa

et al.

Microbiology, Journal Year: 2022, Volume and Issue: 168(5)

Published: May 23, 2022

To prevail in the interaction with eukaryotic hosts, many bacterial pathogens use protein secretion systems to release virulence factors at host–pathogen interface and/or deliver them directly into host cells. An outstanding example of complexity and sophistication diversity their substrates, effectors, is Defective organelle trafficking/Intracellular multiplication (Dot/Icm) Type IVB system (T4BSS) Legionella pneumophila related species. species are facultative intracellular environmental protozoa opportunistic human respiratory pathogens. The Dot/Icm T4BSS translocates an exceptionally large number more than 300 per L. strain, essential for evasion phagolysosomal degradation exploitation macrophages as replicative niches. Recent technological advancements imaging complexes have provided new insight architecture allowed us propose models transport mechanism. At same time, significant progress has been made assigning functions about a third discovering unprecedented enzymatic activities concepts subversion. In this review, we describe current knowledge workings machinery provide overview to-date characterized effectors

Language: Английский

Citations

34
Ubiquitination of non-protein substrates DOI Creative Commons
Jun-Ichi Sakamaki, Noboru Mizushima

Trends in Cell Biology, Journal Year: 2023, Volume and Issue: 33(11), P. 991 - 1003

Published: April 27, 2023

Language: Английский

Citations

20
Ubiquitin‐targeted bacterial effectors: rule breakers of the ubiquitin system DOI
Cameron G. Roberts, Tyler G. Franklin, Jonathan N. Pruneda

et al.

The EMBO Journal, Journal Year: 2023, Volume and Issue: 42(18)

Published: Aug. 9, 2023

Language: Английский

Citations

20
Bacterial DUBs: deubiquitination beyond the seven classes DOI
Thomas Hermanns, Kay Hofmann

Biochemical Society Transactions, Journal Year: 2019, Volume and Issue: 47(6), P. 1857 - 1866

Published: Dec. 17, 2019

Protein ubiquitination is a posttranslational modification that regulates many aspects of cellular life, including proteostasis, vesicular trafficking, DNA repair and NF-κB activation. By directly targeting intracellular bacteria or bacteria-containing vacuoles to the lysosome, also an important component cell-autonomous immunity. Not surprisingly, several pathogenic encode deubiquitinases (DUBs) use them as secreted effectors prevent bacterial components. A systematic overview known DUBs, their cleavage specificities biological roles, suggests multiple independent acquisition events from host-encoded DUBs other proteases. The widely used classification into seven well-defined families should only be applied eukaryotic since do not follow this classification.

Language: Английский

Citations

45
Beyond protein modification: the rise of non-canonical ADP-ribosylation DOI Creative Commons
M. Schuller, Ivan Ahel

Biochemical Journal, Journal Year: 2022, Volume and Issue: 479(4), P. 463 - 477

Published: Feb. 17, 2022

ADP-ribosylation has primarily been known as post-translational modification of proteins. As signalling strategy conserved in all domains life, it modulates substrate activity, localisation, stability or interactions, thereby regulating a variety cellular processes and microbial pathogenicity. Yet over the last years, there is increasing evidence non-canonical forms that are catalysed by certain members ADP-ribosyltransferase family go beyond traditional protein signalling. New macromolecular targets such nucleic acids new ADP-ribose derivatives have established, notably extending repertoire Based on physiological relevance so far, deserves its recognition next to which we therefore review following.

Language: Английский

Citations

27