Conservation and similarity of bacterial and eukaryotic innate immunity

Nature Reviews Microbiology, Год журнала: 2024, Номер 22(7), С. 420 - 434

Опубликована: Фев. 28, 2024

Язык: Английский

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A eukaryotic-like ubiquitination system in bacterial antiviral defence

Nature, Год журнала: 2024, Номер 631(8022), С. 843 - 849

Опубликована: Июль 17, 2024

Язык: Английский

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Capturing dynamic phage-pathogen coevolution by clinical surveillance

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 29, 2025

Abstract Bacteria harness diverse defense systems that protect against phage predation 1 , many of which are encoded on horizontally transmitted mobile genetic elements (MGEs) 2 . In turn, phages evolve counter-defenses 3 driving a dynamic arms race remains underexplored in human disease contexts. For the diarrheal pathogen Vibrio cholerae higher burden its lytic phage, ICP1, patient stool correlates with reduced severity 4 However, direct molecular evidence phage-driven selection epidemic V. has not been demonstrated. Here, through clinical surveillance cholera-endemic Bangladesh, we capture acquisition parasitic anti-phage MGE, PLE11, initiated selective sweep coinciding largest cholera outbreak recent records. PLE11 exhibited potent activity co-circulating explaining rapid and dominating emergence. We identify PLE11-encoded Rta as novel responsible provide restricts tail assembly. Using experimental evolution, predict counteradaptations document eventual emergence ICP1 achieves convergent evolutionary outcome. By probing how PLEs hijack structural proteins to drive their horizontal transmission while simultaneously restricting assembly, discover manipulate assembly construct chimeric tails comprised MGE phage-encoded proteins. Collectively, our findings reveal basis natural globally significant virus clinically relevant context.

Язык: Английский

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Tail assembly interference is a common strategy in bacterial antiviral defenses

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 30, 2024

Many bacterial immune systems recognize phage structural components to activate antiviral responses, without inhibiting the function of component. These can be encoded in specific chromosomal loci, known as defense islands, and mobile genetic elements such prophages phage-inducible islands (PICIs). Here, we identify a family systems, named Tai (for 'tail assembly inhibition'), that is prevalent PICIs, P4-like satellites. protect their host population from other phages by blocking tail step, leading release tailless incapable infecting new hosts. To prevent autoimmunity, some Tai-positive have an associated counter-defense mechanism expressed during lytic cycle allows for formation. Interestingly, genes are organized non-contiguous operon, enabling coordinated expression.

Язык: Английский

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A phage satellite manipulates the viral DNA packaging motor to inhibit phage and promote satellite spread

Nucleic Acids Research, Год журнала: 2024, Номер 52(17), С. 10431 - 10446

Опубликована: Авг. 16, 2024

Abstract ICP1, a lytic bacteriophage of Vibrio cholerae, is parasitized by phage satellites, PLEs, which hijack ICP1 proteins for their own horizontal spread. PLEs' dependence on ICP1’s DNA replication machinery and virion components results in inhibition lifecycle. PLEs are expected to depend factors genome packaging, but the mechanism(s) use inhibit packaging currently unknown. Here, we identify characterize Gpi, PLE’s indiscriminate inhibitor. Gpi binds large terminase (TerL), motor, blocks packaging. To overcome Gpi's negative effect TerL, component PLE also requires, uses two specifiers, GpsA GpsB, that specifically allow genomes. Surprisingly, mimicry pac site as backup strategy ensure mimicry, however, only sufficient if can at other stages its lifecycle, suggesting an advantage maintaining GpsB. Collectively, these provide mechanistic insights into another stage lifecycle inhibited PLE, most inhibitory documented satellites. More broadly, represents first satellite-encoded inhibitor TerL.

Язык: Английский

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A Vibrio cholerae Type IV restriction system targets glucosylated 5-hydroxymethylcytosine to protect against phage infection

Journal of Bacteriology, Год журнала: 2024, Номер 206(9)

Опубликована: Сен. 4, 2024

A major challenge faced by

Язык: Английский

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Overview of Phage Defense Systems in Bacteria and Their Applications

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13316 - 13316

Опубликована: Дек. 12, 2024

As natural parasites of bacteria, phages have greatly contributed to bacterial evolution owing their persistent threat. Diverse phage resistance systems been developed in bacteria during the coevolutionary process with phages. Conversely, contamination has a devastating effect on microbial fermentation, resulting fermentation failure and substantial economic loss. Accordingly, defense derived from can be employed obtain robust phage-resistant host cells that overcome threats posed by bacteriophages industrial processes. In this review, diverse mechanisms, including remarkable research progress potential applications, are systematically summarized. addition, development prospects challenges discussed. This review provides useful reference for developing bacteria.

Язык: Английский

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A prophage-encoded anti-phage defense system that prevents phage DNA packaging by targeting the terminase complex

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

Summary A unique feature of temperate phages is the ability to protect their host bacteria from a second phage infection. Such protection granted at lysogenic state, where persist as prophages integrated within bacterial chromosome, expressing genes that defend and themselves predation. Here, we report prophage-encoded anti-phage defense system inhibits DNA packaging invading in Listeria monocytogenes . This includes protein, TerI, two self-immunity proteins, anti-TerI1 anti-TerI2. TerI targets terminase complex prevent translocation into procapsids without halting lytic cycle, leading release unpacked non-infectious upon lysis. In contrast, anti-TerI2, counteract during prophage induction allow virion production. system, TERi, prevalent phages, providing population-level compromising lifecycle.

Язык: Английский

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Structural diversity and oligomerization of bacterial ubiquitin-like proteins

Structure, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

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Grundlagenwissen zu Phagen und ihrer therapeutischen Anwendung

Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz, Год журнала: 2025, Номер unknown

Опубликована: Май 2, 2025

Zusammenfassung Phagen (Bakteriophagen) sind Viren, die spezifisch Bakterien infizieren und zerstören können. Sie in der Natur weitverbreitet spielen eine wichtige Rolle mikrobiellen Ökosystemen. In Medizin werden sie als mögliche Alternative oder Ergänzung zu Antibiotika erforscht, können zum Beispiel bei Wund‑, Harnwegs- Lungeninfektionen eingesetzt werden. Anwendung finden dabei einzelne sogenannte Phagencocktails. Dieser Übersichtsbeitrag Grundlagenwissen beleuchtet altbekannte Stichworte aus dem Wissen Phagenbiologie moderne Zusammenhänge Forschungsschwerpunkte stellt Wirkmechanismen von Grundlage für therapeutische vor. Dabei wird vor allem auf Phage-Wirt-Interaktion, den Mechanismus Lyse, Morphologie sowie spezifische Methoden zur visuellen Darstellung eingegangen. Als Teil des menschlichen Mikrobioms tragen Schleimhäuten (Mukosa) Immunabwehr bei. Auch therapeutisch nicht einsetzbaren temperenten vorgestellt, Prophagen im bakteriellen Genom verweilen können, letzten Jahren entdeckten CrAss-Phagen (Crassvirales) Lak-Megaphagen. Die bakterielle Phagenabwehr, Phagenresistenz Phagen-Antibiotika-Synergien weitere Themen. Ein Ausblick zukünftige Forschung gegeben, Bedeutung koordinierten Sammlung Forschungsergebnissen herausgestellt. sollen ersetzen, deren Effizienz sogar wieder verbessern. Zulassungsprozesse Phagentherapie aktuell noch herausfordernd. Das Vertrauen Phagenpräparate muss Qualität bauen durch harmonisierte Standards garantieren ist.

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