Multi-conflict islands are a widespread trend within Serratia spp. DOI Creative Commons

Thomas Cummins,

Suraj Songra,

S Garrett

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(12), P. 115055 - 115055

Published: Dec. 1, 2024

Language: Английский

Conservation and similarity of bacterial and eukaryotic innate immunity DOI
Hannah E. Ledvina, Aaron T. Whiteley

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(7), P. 420 - 434

Published: Feb. 28, 2024

Language: Английский

Citations

24
A eukaryotic-like ubiquitination system in bacterial antiviral defence DOI
Lydia R. Chambers, Qiaozhen Ye, Jiaxi Cai

et al.

Nature, Journal Year: 2024, Volume and Issue: 631(8022), P. 843 - 849

Published: July 17, 2024

Language: Английский

Citations

9
Capturing dynamic phage-pathogen coevolution by clinical surveillance DOI Creative Commons
Yamini Mathur, Caroline M. Boyd,

Jeannette E. Farnham

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract Bacteria harness diverse defense systems that protect against phage predation 1 , many of which are encoded on horizontally transmitted mobile genetic elements (MGEs) 2 . In turn, phages evolve counter-defenses 3 driving a dynamic arms race remains underexplored in human disease contexts. For the diarrheal pathogen Vibrio cholerae higher burden its lytic phage, ICP1, patient stool correlates with reduced severity 4 However, direct molecular evidence phage-driven selection epidemic V. has not been demonstrated. Here, through clinical surveillance cholera-endemic Bangladesh, we capture acquisition parasitic anti-phage MGE, PLE11, initiated selective sweep coinciding largest cholera outbreak recent records. PLE11 exhibited potent activity co-circulating explaining rapid and dominating emergence. We identify PLE11-encoded Rta as novel responsible provide restricts tail assembly. Using experimental evolution, predict counteradaptations document eventual emergence ICP1 achieves convergent evolutionary outcome. By probing how PLEs hijack structural proteins to drive their horizontal transmission while simultaneously restricting assembly, discover manipulate assembly construct chimeric tails comprised MGE phage-encoded proteins. Collectively, our findings reveal basis natural globally significant virus clinically relevant context.

Language: Английский

Citations

1
Tail assembly interference is a common strategy in bacterial antiviral defenses DOI Creative Commons
Lingchen He, Laura Miguel-Romero, Jonasz B. Patkowski

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 30, 2024

Many bacterial immune systems recognize phage structural components to activate antiviral responses, without inhibiting the function of component. These can be encoded in specific chromosomal loci, known as defense islands, and mobile genetic elements such prophages phage-inducible islands (PICIs). Here, we identify a family systems, named Tai (for 'tail assembly inhibition'), that is prevalent PICIs, P4-like satellites. protect their host population from other phages by blocking tail step, leading release tailless incapable infecting new hosts. To prevent autoimmunity, some Tai-positive have an associated counter-defense mechanism expressed during lytic cycle allows for formation. Interestingly, genes are organized non-contiguous operon, enabling coordinated expression.

Language: Английский

Citations

5
A prophage-encoded anti-phage defense system that prevents phage DNA packaging by targeting the terminase complex DOI Creative Commons

Gil Azulay,

Nadejda Sigal,

Keren Tzohar Rabinovich

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Summary A unique feature of temperate phages is the ability to protect their host bacteria from a second phage infection. Such protection granted at lysogenic state, where persist as prophages integrated within bacterial chromosome, expressing genes that defend and themselves predation. Here, we report prophage-encoded anti-phage defense system inhibits DNA packaging invading in Listeria monocytogenes . This includes protein, TerI, two self-immunity proteins, anti-TerI1 anti-TerI2. TerI targets terminase complex prevent translocation into procapsids without halting lytic cycle, leading release unpacked non-infectious upon lysis. In contrast, anti-TerI2, counteract during prophage induction allow virion production. system, TERi, prevalent phages, providing population-level compromising lifecycle.

Language: Английский

Citations

0
Structural diversity and oligomerization of bacterial ubiquitin-like proteins DOI

Minheng Gong,

Qiaozhen Ye, Yajie Gu

et al.

Structure, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
A phage satellite manipulates the viral DNA packaging motor to inhibit phage and promote satellite spread DOI Creative Commons
Caroline M. Boyd, Kimberley D. Seed

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 52(17), P. 10431 - 10446

Published: Aug. 16, 2024

Abstract ICP1, a lytic bacteriophage of Vibrio cholerae, is parasitized by phage satellites, PLEs, which hijack ICP1 proteins for their own horizontal spread. PLEs' dependence on ICP1’s DNA replication machinery and virion components results in inhibition lifecycle. PLEs are expected to depend factors genome packaging, but the mechanism(s) use inhibit packaging currently unknown. Here, we identify characterize Gpi, PLE’s indiscriminate inhibitor. Gpi binds large terminase (TerL), motor, blocks packaging. To overcome Gpi's negative effect TerL, component PLE also requires, uses two specifiers, GpsA GpsB, that specifically allow genomes. Surprisingly, mimicry pac site as backup strategy ensure mimicry, however, only sufficient if can at other stages its lifecycle, suggesting an advantage maintaining GpsB. Collectively, these provide mechanistic insights into another stage lifecycle inhibited PLE, most inhibitory documented satellites. More broadly, represents first satellite-encoded inhibitor TerL.

Language: Английский

Citations

3
A Vibrio cholerae Type IV restriction system targets glucosylated 5-hydroxymethylcytosine to protect against phage infection DOI
Jasper B. Gomez, Christopher M. Waters

Journal of Bacteriology, Journal Year: 2024, Volume and Issue: 206(9)

Published: Sept. 4, 2024

A major challenge faced by

Language: Английский

Citations

2
Overview of Phage Defense Systems in Bacteria and Their Applications DOI Open Access

Xiaomei Xu,

Pengfei Gu

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13316 - 13316

Published: Dec. 12, 2024

As natural parasites of bacteria, phages have greatly contributed to bacterial evolution owing their persistent threat. Diverse phage resistance systems been developed in bacteria during the coevolutionary process with phages. Conversely, contamination has a devastating effect on microbial fermentation, resulting fermentation failure and substantial economic loss. Accordingly, defense derived from can be employed obtain robust phage-resistant host cells that overcome threats posed by bacteriophages industrial processes. In this review, diverse mechanisms, including remarkable research progress potential applications, are systematically summarized. addition, development prospects challenges discussed. This review provides useful reference for developing bacteria.

Language: Английский

Citations

2
Bacterial antiviral defense pathways encode eukaryotic-like ubiquitination systems DOI Creative Commons
Lydia R. Chambers, Qiaozhen Ye, Jiaxi Cai

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 26, 2023

Ubiquitination and related pathways play crucial roles in protein homeostasis, signaling, innate immunity

Language: Английский

Citations

4