ACS Omega,
Год журнала:
2023,
Номер
8(30), С. 26806 - 26815
Опубликована: Июль 18, 2023
Isoprene
is
a
valuable
platform
chemical,
which
produced
by
engineered
microorganisms,
albeit
in
low
quantities.
The
amount
of
isoprene
usually
measured
gas
chromatography,
can
be
time-consuming
and
expensive.
Alternatively,
biosensors
have
evolved
as
powerful
tool
for
real-time
high-throughput
screening
monitoring
product
synthesis.
AraC-pBAD-inducible
system
has
been
widely
studied,
evolved,
to
develop
small
molecules.
In
our
preliminary
studies,
the
AraC-pBAD
was
mildly
induced
at
higher
concentrations
when
arabinose
also
available.
Hence,
present
study,
we
designed
constructed
synthetic
biosensor
based
on
system,
wherein
ligand-binding
domain
AraC
replaced
with
IsoA.
On
introducing
this
chimeric
AraC-IsoA
(AcIa)
transcription
factor
native
PBAD
promoter
regulating
rfp
gene
expression,
fluorescence
output
observed
only
wild-type
Escherichia
coli
cells
were
both
arabinose.
sensitivity
dynamic
range
further
enhanced
removing
operator
sequences
substituting
(PAraC)
strong
tac
(Ptac).
sensor
did
not
work
knockout
strains;
however,
functionality
restored
reintroducing
AraC.
essential
functioning
biosensor,
while
AcIa
provides
specificity
isoprene.
However,
insights
into
how
AraC-AcIa
interacts
possible
working
mechanism
remain
explored.
This
study
prototype
developing
AraC-based
proteins
devoid
known
dimerizing
domains
opens
new
avenue
exploration.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Фев. 23, 2023
Antibiotics
are
a
key
control
mechanism
for
synthetic
biology
and
microbiology.
Resistance
genes
used
to
select
desired
cells
regulate
bacterial
populations,
however
their
use
to-date
has
been
largely
static.
Precise
spatiotemporal
of
antibiotic
resistance
could
enable
wide
variety
applications
that
require
dynamic
susceptibility
survival.
Here,
we
light-inducible
Cre
recombinase
activate
expression
drug
in
Escherichia
coli.
We
demonstrate
light-activated
four
antibiotics:
carbenicillin,
kanamycin,
chloramphenicol,
tetracycline.
Cells
exposed
blue
light
survive
the
presence
lethal
concentrations,
while
those
kept
dark
do
not.
To
optimize
induction,
vary
promoter,
ribosome
binding
site,
enzyme
variant
strength
using
chromosome
plasmid-based
constructs.
then
link
inducible
heterologous
fatty
acid
increase
production
octanoic
acid.
These
optogenetic
tools
pave
way
cell
Bioengineering,
Год журнала:
2020,
Номер
7(4), С. 151 - 151
Опубликована: Ноя. 24, 2020
Progress
in
metabolic
engineering
and
synthetic
systems
biology
has
made
bioproduction
an
increasingly
attractive
competitive
strategy
for
synthesizing
biomolecules,
recombinant
proteins
biofuels
from
renewable
feedstocks.
Yet,
due
to
poor
productivity,
it
remains
difficult
make
a
process
economically
viable
at
large
scale.
Achieving
dynamic
control
of
cellular
processes
could
lead
even
better
yields
by
balancing
the
two
characteristic
phases
bioproduction,
namely,
growth
versus
production,
which
lie
heart
trade-off
that
substantially
impacts
productivity.
The
versatility
controllability
offered
light
will
be
key
element
attaining
level
desired.
popularity
light-mediated
is
increasing,
with
expanding
repertoire
optogenetic
novel
applications,
many
devices
have
been
designed
test
strains
various
culture
scales
objectives.
In
this
review,
we
aim
highlight
most
important
advances
direction.
We
discuss
how
optogenetics
currently
applied
metabolism
context
describe
instruments
used
laboratory
scale
strain
development,
explore
current
industrial-scale
adapted
or
benefit
existing
photobioreactor
designs.
then
draw
attention
steps
must
undertaken
further
optimize
biological
order
take
full
advantage
potential
microbial
factories.
FEMS Microbiology Reviews,
Год журнала:
2021,
Номер
46(2)
Опубликована: Ноя. 11, 2021
ABSTRACT
Optogenetics
holds
the
promise
of
controlling
biological
processes
with
superb
temporal
and
spatial
resolution
at
minimal
perturbation.
Although
many
light-reactive
proteins
used
in
optogenetic
systems
are
derived
from
prokaryotes,
applications
were
largely
limited
to
eukaryotes
for
a
long
time.
In
recent
years,
however,
an
increasing
number
microbiologists
use
optogenetics
as
powerful
new
tool
study
control
key
aspects
bacterial
biology
fast
often
reversible
manner.
After
brief
discussion
principles,
this
review
provides
overview
rapidly
growing
bacteria,
particular
focus
on
studies
venturing
beyond
transcriptional
control.
To
guide
future
experiments,
we
highlight
helpful
tools,
provide
considerations
successful
application
systems,
identify
opportunities
challenges
that
arise
when
applying
these
approaches
bacteria.
ACS Applied Materials & Interfaces,
Год журнала:
2022,
Номер
14(41), С. 46351 - 46361
Опубликована: Окт. 6, 2022
Engineering
bacteria
can
achieve
targeted
and
controllable
cancer
therapy
using
synthetic
biology
technology
the
characteristics
of
tumor
microenvironment.
Besides,
accurate
diagnosis
visualization
treatment
process
are
also
vital
for
bacterial
therapy.
In
this
paper,
a
light
control
engineered
system
based
on
upconversion
nanoparticles
(UCNP)-mediated
time-resolved
imaging
(TRI)
was
constructed
colorectal
theranostic
UCNP
with
different
luminous
lifetimes
were
separately
modified
targeting
molecule
(folic
acid)
or
anaerobic
(Nissle
1917,
EcN)
to
realize
co-localization
tissues,
thus
improving
diagnostic
accuracy
TRI.
addition,
blue
used
induce
(EcN-pDawn-φx174E/TRAIL)
lysis
release
apoptosis-related
inducing
ligand
(TRAIL),
triggering
cell
death.
vitro
in
vivo
results
indicated
that
could
light-controlled
EcN-pDawn-φx174E/TRAIL
irradiation
inhibit
53%
growth
comparison
without
(11.8%).
We
expect
provides
new
intelligent
construction
theranostics.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 23, 2024
Oscillations
are
a
recurrent
phenomenon
in
biological
systems
across
scales,
but
deciphering
their
fundamental
principles
is
very
challenging.
Here,
we
tackle
this
challenge
by
redesigning
the
wellcharacterised
synthetic
oscillator
known
as
"repressilator"
Escherichia
coli
and
controlling
it
using
optogenetics,
creating
"optoscillator".
Bacterial
colonies
manifest
oscillations
spatial
ring
patterns.
When
apply
periodic
light
pulses,
optoscillator
behaves
forced
systematically
investigate
properties
of
rings
under
various
conditions.
Combining
experiments
with
mathematical
modeling,
demonstrate
that
simple
oscillatory
circuit
can
generate
complex
dynamics
transformed
into
distinct
We
report
observation
synchronisation,
resonance,
subharmonic
resonance
period
doubling.
Furthermore,
present
evidence
chaotic
regime.
This
work
highlights
intricate
spatiotemporal
patterns
accessible
oscillators
underscores
potential
our
approach
revealing
oscillations.
Here
authors
construct
bacteria
be
controlled
show
different
conditions
bacterial
colonies.
