A 3D whole-face movement analysis system to uncover underlying physiology in mice DOI Creative Commons
Kyle Daruwalla,

I MARTIN,

Linghua Zhang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 8, 2024

Abstract Facial expressions and movements, from a subtle ephemeral grimace, to vigorous rapid chewing, offer direct insights into the moment-to-moment change of neural physiological processes. Mice, with discernible facial responses evolutionarily conserved mammalian movement control circuits, provide an ideal model unravel link between underlying states. However, existing frameworks lack spatial or temporal resolution sensitively track all movements mouse face, due its small conical form factor. We introduce Cheese3D, computer vision system that captures high-speed 3D motion entire face (including ears, eyes, whisker pad, jaw, while covering both sides face) using calibrated six-camera array. The interpretable framework extracts dynamics anatomically-meaningful features in absolute world units at sub-millimeter precision. precise face-wide data generated by Cheese3D provides clear insights, as shown proof-of-principle experiments predicting time under general anesthesia changing patterns, inferring tooth muscle anatomy fast ingestion motions across measuring minute differences evoked brainstem stimulation, relating activity spontaneous including expressive only measurable (e.g., angles ear motion). can serve discovery tool renders highly readout otherwise hidden internal

Язык: Английский

Cholinergic and noradrenergic axonal activity contains a behavioral-state signal that is coordinated across the dorsal cortex DOI Creative Commons
Lindsay Collins,

John Francis,

Brett Emanuel

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Апрель 27, 2023

Fluctuations in brain and behavioral state are supported by broadly projecting neuromodulatory systems. In this study, we use mesoscale two-photon calcium imaging to examine spontaneous activity of cholinergic noradrenergic axons awake mice order determine the interaction between arousal/movement transitions across dorsal cortex at distances separated up 4 mm. We confirm that GCaMP6s within axonal projections both basal forebrain locus coeruleus neurons track arousal, indexed as pupil diameter, changes engagement, reflected bouts whisker movement and/or locomotion. The broad coordination even distant segments indicates these systems can communicate, part, through a global signal, especially relation state. addition coordinated activity, also find evidence subpopulation may exhibit heterogeneity appears be independent our measures By monitoring interneurons cortex, found cells state-dependent (arousal/movement) activity. These results demonstrate provide prominent synchronized signal related state, therefore contribute cortical excitability.

Язык: Английский

Процитировано

54
Vagal sensory neurons mediate the Bezold–Jarisch reflex and induce syncope DOI Creative Commons
Jonathan W. Lovelace,

Jingrui Ma,

Saurabh Yadav

и другие.

Nature, Год журнала: 2023, Номер 623(7986), С. 387 - 396

Опубликована: Ноя. 1, 2023

Visceral sensory pathways mediate homeostatic reflexes, the dysfunction of which leads to many neurological disorders

Язык: Английский

Процитировано

41
Rapid fluctuations in functional connectivity of cortical networks encode spontaneous behavior DOI
Hadas Benisty, Daniel Barson,

Andrew H. Moberly

и другие.

Nature Neuroscience, Год журнала: 2023, Номер 27(1), С. 148 - 158

Опубликована: Ноя. 30, 2023

Язык: Английский

Процитировано

35
Cortical reactivations predict future sensory responses DOI
Nghia D. Nguyen, Andrew Lutas, Oren Amsalem

и другие.

Nature, Год журнала: 2023, Номер 625(7993), С. 110 - 118

Опубликована: Дек. 13, 2023

Язык: Английский

Процитировано

24
Lightning Pose: improved animal pose estimation via semi-supervised learning, Bayesian ensembling and cloud-native open-source tools DOI
Dan Biderman, Matthew R Whiteway, Cole Hurwitz

и другие.

Nature Methods, Год журнала: 2024, Номер 21(7), С. 1316 - 1328

Опубликована: Июнь 25, 2024

Язык: Английский

Процитировано

12
Dynamic corticothalamic modulation of the somatosensory thalamocortical circuit during wakefulness DOI Creative Commons
Elaida Dimwamwa, Aurélie Pala, Vivek Chundru

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 25, 2024

The feedback projections from cortical layer 6 (L6CT) to the sensory thalamus have long been implicated in playing a primary role gating signaling but remain poorly understood. To causally elucidate full range of effects these projections, we targeted silicon probe recordings whisker thalamocortical circuit awake mice selectively expressing Channelrhodopsin-2 L6CT neurons. Through optogenetic manipulation neurons, multi-site electrophysiological recordings, and modeling circuitry, establish neurons as dynamic modulators ongoing spiking ventral posteromedial nucleus (VPm), either suppressing or enhancing VPm depending on neurons' firing rate synchrony. Differential across excitatory inhibitory sub-populations point an overall influence excitability that could profound implications for regulating ethologically relevant conditions.

Язык: Английский

Процитировано

8
Rastermap: a discovery method for neural population recordings DOI Creative Commons
Carsen Stringer, Lin Zhong, Atika Syeda

и другие.

Nature Neuroscience, Год журнала: 2024, Номер unknown

Опубликована: Окт. 16, 2024

Abstract Neurophysiology has long progressed through exploratory experiments and chance discoveries. Anecdotes abound of researchers listening to spikes in real time noticing patterns activity related ongoing stimuli or behaviors. With the advent large-scale recordings, such close observation data become difficult. To find neural data, we developed ‘Rastermap’, a visualization method that displays neurons as raster plot after sorting them along one-dimensional axis based on their patterns. We benchmarked Rastermap realistic simulations then used it explore recordings tens thousands from mouse cortex during spontaneous, stimulus-evoked task-evoked epochs. also applied whole-brain zebrafish recordings; wide-field imaging data; electrophysiological rat hippocampus, monkey frontal various cortical subcortical regions mice; artificial networks. Finally, illustrate high-dimensional scenarios where similar algorithms cannot be effectively.

Язык: Английский

Процитировано

8
Probabilistically constrained vector summation of motion direction in the mouse superior colliculus DOI

Chuiwen Li,

Victor J. DePiero,

Hui Chen

и другие.

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Detection and neural encoding of whisker-generated sounds in mice DOI

B.G. Efron,

Athanasios Ntelezos,

Yonatan Katz

и другие.

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1
Distinct streams for supervised and unsupervised learning in the visual cortex DOI Creative Commons
Lin Zhong,

Scott Baptista,

Rachel Gattoni

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 27, 2024

Representation learning in neural networks may be implemented with supervised or unsupervised algorithms, distinguished by the availability of feedback. In sensory cortex, perceptual drives plasticity, but it is not known if this due to learning. Here we recorded populations up 90,000 neurons simultaneously from primary visual cortex (V1) and higher areas (HVA), while mice learned multiple tasks as well during unrewarded exposure same stimuli. Similar previous studies, found that changes task were correlated their behavioral However, mostly replicated exposure, suggesting fact The plasticity was concentrated medial HVAs obeyed visual, rather than spatial, rules. only, a ramping reward prediction signal anterior HVAs, potentially involved Our results predict accelerate subsequent learning, which validated experiments.

Язык: Английский

Процитировано

6