Development and applications of enzymatic peptide and protein ligation DOI
Yan Cui, Dongyang Han, Xinfeng Bai

и другие.

Journal of Peptide Science, Год журнала: 2024, Номер unknown

Опубликована: Окт. 21, 2024

Chemical synthesis of complex peptides and proteins continues to play increasingly important roles in industry academia, where strategies for covalent ligation two or more peptide fragments produce longer convergent manners have become critical. In recent decades, efficient site‐selective mediated by exploiting the biocatalytic capacity nature's diverse toolkit (i.e., enzymes) been widely recognized as a powerful extension existing chemical strategies. this review, we present chronological overview development proteases, transpeptidases, transglutaminases, ubiquitin ligases. We survey different properties between reactions various enzymes, including selectivity efficiency reaction, “scar” left product, type amide bond formed (natural isopeptide), synthetic availability reactants, whether enzymes are orthogonal another. This review also describes how inherent specificity these can be exploited protein ligation.

Язык: Английский

Chemical Synthesis of Human Proteoforms and Application in Biomedicine DOI Creative Commons
Huasong Ai, Man Pan, Lei Liu

и другие.

ACS Central Science, Год журнала: 2024, Номер 10(8), С. 1442 - 1459

Опубликована: Июль 22, 2024

Limited understanding of human proteoforms with complex posttranslational modifications and the underlying mechanisms poses a major obstacle to research on health disease. This Outlook discusses opportunities challenges

Язык: Английский

Процитировано

11
The Emerging Role of the Histone H2AK13/15 Ubiquitination: Mechanisms of Writing, Reading, and Erasing in DNA Damage Repair and Disease DOI Creative Commons

Qi Shu,

Yun Liu, Huasong Ai

и другие.

Cells, Год журнала: 2025, Номер 14(4), С. 307 - 307

Опубликована: Фев. 18, 2025

Histone modifications serve as molecular switches controlling critical cellular processes. The ubiquitination of histone H2A at lysines 13 and 15 (H2AK13/15ub) is a crucial epigenetic modification that coordinates DNA repair genome stability during the damage response (DDR). This mark dynamically regulated by three functional protein groups: "writer" enzymes (e.g., E3 ubiquitin ligase RNF168 catalyzes H2AK13/15ub formation), "reader" proteins (including 53BP1 BRCA1-BARD1 recognize to guide repair), "eraser" deubiquitinases (such USP3 USP16 remove modification). Dysregulation precisely coordinated network strongly associated with various diseases, including RIDDLE syndrome, neurodegenerative disorders, immune deficiencies, breast cancer. review systematically analyzes dynamic regulation in DDR explores its therapeutic potential for disease intervention.

Язык: Английский

Процитировано

0
Advances in the chemical synthesis of human proteoforms DOI
Ziyi Yang, Yingqi Xiao, Yang Shi

и другие.

Science China Life Sciences, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

Язык: Английский

Процитировано

0
Examining the Role of Threonine Phosphorylation in Ubiquitin’s Function Using Chemical Protein Synthesis DOI Creative Commons

B J Wang,

Chuntong Li, Feng Zhao

и другие.

JACS Au, Год журнала: 2025, Номер unknown

Опубликована: Апрель 21, 2025

Язык: Английский

Процитировано

0
Mechanism of USP21 autoinhibition and histone H2AK119 deubiquitination DOI Creative Commons
Sanim Rahman,

Michael T. Morgan,

Chad W. Hicks

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 21, 2025

Abstract Monoubiquitinated histone H2A lysine 119 (H2AK119ub) is a signature modification associated with transcriptional silencing and heterochromatin formation. Ubiquitin-specific protease 21 (USP21), one of four major deubiquitinating enzymes (DUBs) that target H2AK119ub, plays critical roles in diverse cellular processes 1–4 . The molecular mechanisms by which USP21 specifically deubiquitinates H2AK119ub regulated unknown. contains C-terminal USP catalytic domain, preceded an N-terminal intrinsically disordered region (IDR). We determined the cryo-EM structure domain bound to nucleosome, reveals recognition mode differs from two other H2AK119-specific DUBs, Polycomb repressive complex 5 USP16 6 unexpectedly discovered (IDR) inhibits enzyme’s activity. Using AlphaFold-Multimer perform virtual screen interactors, we identified kinases phosphorylate IDR thereby relieve autoinhibition. Modeling using AlphaFold3 suggests structural model explaining mechanism AlphaFold analysis ubiquitin-specific proteases phosphorylation-regulated autoinhibition may be feature multiple enzymes. These findings shed light on H2AK119 deubiquitination reveal novel phosphorylation-dependent DUB autoregulation.

Язык: Английский

Процитировано

0
Structure of the poxvirus core DOI
Fasséli Coulibaly

Nature Structural & Molecular Biology, Год журнала: 2024, Номер 31(7), С. 1001 - 1003

Опубликована: Июнь 18, 2024

Язык: Английский

Процитировано

2
Development and applications of enzymatic peptide and protein ligation DOI
Yan Cui, Dongyang Han, Xinfeng Bai

и другие.

Journal of Peptide Science, Год журнала: 2024, Номер unknown

Опубликована: Окт. 21, 2024

Chemical synthesis of complex peptides and proteins continues to play increasingly important roles in industry academia, where strategies for covalent ligation two or more peptide fragments produce longer convergent manners have become critical. In recent decades, efficient site‐selective mediated by exploiting the biocatalytic capacity nature's diverse toolkit (i.e., enzymes) been widely recognized as a powerful extension existing chemical strategies. this review, we present chronological overview development proteases, transpeptidases, transglutaminases, ubiquitin ligases. We survey different properties between reactions various enzymes, including selectivity efficiency reaction, “scar” left product, type amide bond formed (natural isopeptide), synthetic availability reactants, whether enzymes are orthogonal another. This review also describes how inherent specificity these can be exploited protein ligation.

Язык: Английский

Процитировано

0