Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens
Veterinary Quarterly,
Год журнала:
2025,
Номер
45(1), С. 1 - 13
Опубликована: Янв. 30, 2025
Salmonella
Pullorum,
the
causative
agent
of
pullorum
disease,
posing
a
significant
threat
to
global
production
poultry
meat
and
eggs.
However,
existing
detection
methods
have
substantial
limitations
in
efficiency
accuracy.
Herein,
we
developed
genomic
deletion-targeted
TaqMan
qPCR
assay
for
identification
enabling
precise
differentiation
from
other
serovars.
The
assay's
limit
was
5
copies/μL
plasmid
4
CFU/μL
bacterial
DNA.
Furthermore,
collected
676
chicken
samples
an
established
infection
model
compare
performance
with
traditional
culturing
antibody-based
approaches.
With
superior
sensitivity
specificity,
newly
method
detected
over
80%
positive
chickens,
significantly
outperforming
two
conventional
methods.
Moreover,
proposed
combined
framework
that
incorporates
advantages
antibody
method,
further
enhancing
rate
positives
92%.
Additionally,
address
frequent
aerosol
contamination
amplification
products
laboratory
settings,
devised
easy-to-deploy
anti-contamination
system
based
on
T7
exonuclease.
Overall,
exonuclease-assisted
will
not
only
upgrade
current
but
also
exemplify
feasibility
targeting
specific
deletions
pathogen
detection.
Язык: Английский
A dynamic platform for global pullorum disease and fowl typhoid
Animal Diseases,
Год журнала:
2024,
Номер
4(1)
Опубликована: Окт. 30, 2024
Abstract
Pullorum
disease
and
fowl
typhoid
are
caused
by
the
Salmonella
serovars
Gallinarum
biovars
Gallinarum,
respectively.
The
prevalence
of
these
diseases
varies
across
regions
is
affected
different
risk
factors
that
remain
insufficiently
documented.
To
fill
this
knowledge
gap,
we
have
compiled
a
global
dataset
for
its
prevalence,
drawing
upon
collection
literature
from
last
seven
decades
obtained
bilingual
databases.
However,
more
interactive
dynamic
platform
still
needed
both
academics
policymakers
to
improve
biosecurity
measures,
limit
transmission,
prevent
future
outbreaks
at
local
levels.
Here,
developed
an
advanced
visualization
depict
especially
in
China,
which
categorized
geographical
region
temporal
span.
offers
user-friendly,
efficient,
visually
engaging
tool
explore
pullorum
between
1945
2021
regions.
Additionally,
allows
users
understand
influence
various
factors,
such
as
breed,
farm
mode,
economic
usage
even
sex
primary
host,
chickens,
on
disease.
We
further
provided
detailed
overview
individual
province
within
China.
In
particular,
selecting
two
provinces
interface,
can
quickly
visualize
grasp
disparities
chosen
This
toolkit
enables
exploration
patterns
contributing
Gallinarum.
freely
available
open
source
http://139.9.85.208/
.
Язык: Английский
Avian-specific Salmonella transition to endemicity is accompanied by localized resistome and mobilome interaction
Опубликована: Фев. 18, 2025
Bacterial
regional
demonstration
after
global
dissemination
is
an
essential
pathway
for
selecting
distinct
finesses.
However,
the
evolution
of
resistome
during
transition
to
endemicity
remains
unaddressed.
Using
most
comprehensive
whole-genome
sequencing
dataset
Salmonella
enterica
serovar
Gallinarum
(
S
.
Gallinarum)
collected
from
15
countries,
including
45
newly
recovered
samples
two
related
local
regions,
we
established
relationship
among
avian-specific
pathogen
genetic
profiles
and
localization
patterns.
Initially,
revealed
international
transmission
evolutionary
history
recent
through
phylogenetic
analysis
conducted
using
a
spatiotemporal
Bayesian
framework.
Our
findings
indicate
that
independent
acquisition
via
mobilome,
primarily
plasmids
transposons
shapes
unique
antimicrobial
resistance
profile
different
lineages.
Notably,
mobilome-resistome
combination
lineages
exhibits
geographical-specific
manner,
further
supporting
localized
endemic
mobilome-driven
process.
Collectively,
this
study
elucidates
adaptation
in
pathogen,
likely
driven
by
farming
style,
provides
valuable
insights
targeted
interventions.
Язык: Английский
Avian-specific Salmonella transition to endemicity is accompanied by localized resistome and mobilome interaction
eLife,
Год журнала:
2025,
Номер
13
Опубликована: Март 4, 2025
Bacterial
regional
demonstration
after
global
dissemination
is
an
essential
pathway
for
selecting
distinct
finesses.
However,
the
evolution
of
resistome
during
transition
to
endemicity
remains
unaddressed.
Using
most
comprehensive
whole-genome
sequencing
dataset
Salmonella
enterica
serovar
Gallinarum
(
S
.
Gallinarum)
collected
from
15
countries,
including
45
newly
recovered
samples
two
related
local
regions,
we
established
relationship
among
avian-specific
pathogen
genetic
profiles
and
localization
patterns.
Initially,
revealed
international
transmission
evolutionary
history
recent
through
phylogenetic
analysis
conducted
using
a
spatiotemporal
Bayesian
framework.
Our
findings
indicate
that
independent
acquisition
via
mobilome,
primarily
plasmids
transposons,
shapes
unique
antimicrobial
resistance
profile
different
lineages.
Notably,
mobilome-resistome
combination
lineages
exhibits
geographical-specific
manner,
further
supporting
localized
endemic
mobilome-driven
process.
Collectively,
this
study
elucidates
adaptation
in
pathogen,
likely
driven
by
farming
style,
provides
valuable
insights
targeted
interventions.
Язык: Английский
An adult chicken mortality case investigation: coinfection by Salmonella Indiana and Kentucky
Animal Diseases,
Год журнала:
2025,
Номер
5(1)
Опубликована: Апрель 27, 2025
Abstract
Coinfection,
the
simultaneous
invasion
of
multiple
pathogens
into
a
single
host,
is
critical
but
understudied
area,
especially
in
farm
animal
sector.
We
report
unique
and
unusual
fatal
case
coinfection
with
S
.
Indiana
Kentucky,
which
has
rarely
been
studied
literature
could
hold
potential
importance
for
veterinary
clinics.
In
silico
analysis
revealed
that
all
isolates
exhibited
extensive
multidrug
resistance.
By
analyzing
plasmids,
two
replicons,
IncHI2
IncHI2A,
were
detected
Indiana,
whereas
no
plasmids
Kentucky.
Chicken
embryo
lethality
assays
demonstrated
both
Kentucky
caused
100%
mortality
by
third
day
post
infection,
significantly
exceeding
control
strains.
These
findings
emphasize
high
pathogenic
these
serovars,
carries
cdtB
gene
encoding
typhoid
toxin,
further
confirming
its
increased
pathogenicity.
Overall,
our
results
underscore
urgent
need
to
improve
biosecurity
measures
mitigate
risk
coinfections
involving
multidrug-resistant
Salmonella
strains
poultry
production
environments.
Язык: Английский
Avian-restrict Salmonella transition to endemicity is accompanied by localized resistome adaptation
eLife,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 10, 2024
Bacterial
regional
demonstration
after
global
dissemination
is
an
essential
pathway
for
selecting
distinct
finesses.
However,
the
evolution
of
resistome
during
transition
to
endemicity
remains
unaddressed.
Using
most
comprehensive
whole-genome
sequencing
dataset
Salmonella
enterica
serovar
Gallinarum
(
S
.
Gallinarum)
collected
from
15
countries,
including
45
newly
recovered
samples
two
related
local
regions,
we
established
relationship
among
avian-specific
pathogen
genetic
profiles
and
localization
patterns.
Initially,
revealed
international
transmission
evolutionary
history
recent
through
phylogenetic
analysis
conducted
using
a
spatiotemporal
Bayesian
framework.
Our
findings
indicate
that
independent
acquisition
via
mobilome,
primarily
plasmids
transposons
shapes
unique
antimicrobial
resistance
profile
different
lineages.
Notably,
mobilome-resistome
combination
lineages
exhibits
geographical-specific
manner,
further
supporting
localized
endemic
mobilome-driven
process.
Collectively,
this
study
elucidates
adaptation
in
pathogen,
likely
driven
by
farming
style,
provides
valuable
insights
targeted
interventions.
Язык: Английский
Avian-specificSalmonella entericaSerovar Gallinarum transition to endemicity is accompanied by localized resistome and mobilome interaction
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 26, 2024
Bacterial
regional
demonstration
after
global
dissemination
is
an
essential
pathway
for
selecting
distinct
finesses.
However,
the
evolution
of
resistome
during
transition
to
endemicity
remains
unaddressed.
Using
most
comprehensive
whole-genome
sequencing
dataset
Salmonella
enterica
serovar
Gallinarum
(S.
Gallinarum)
collected
from
15
countries,
including
45
newly
recovered
samples
two
related
local
regions,
we
established
relationship
among
avian-specific
pathogen
genetic
profiles
and
localization
patterns.
Initially,
revealed
international
transmission
evolutionary
history
S.
recent
through
phylogenetic
analysis
conducted
using
a
spatiotemporal
Bayesian
framework.
Our
findings
indicate
that
independent
acquisition
via
mobilome,
primarily
plasmids
transposons
shapes
unique
antimicrobial
resistance
profile
different
lineages.
Notably,
mobilome-resistome
combination
lineages
exhibits
geographical-specific
manner,
further
supporting
localized
endemic
mobilome-driven
process.
Collectively,
this
study
elucidates
adaptation
in
pathogen,
likely
driven
by
farming
style,
provides
valuable
insights
targeted
interventions.
Язык: Английский
Avian-specific Salmonella enterica Serovar Gallinarum transition to endemicity is accompanied by localized resistome and mobilome interaction
Опубликована: Дек. 18, 2024
Bacterial
regional
demonstration
after
global
dissemination
is
an
essential
pathway
for
selecting
distinct
finesses.
However,
the
evolution
of
resistome
during
transition
to
endemicity
remains
unaddressed.
Using
most
comprehensive
whole-genome
sequencing
dataset
Salmonella
enterica
serovar
Gallinarum
(
S
.
Gallinarum)
collected
from
15
countries,
including
45
newly
recovered
samples
two
related
local
regions,
we
established
relationship
among
avian-specific
pathogen
genetic
profiles
and
localization
patterns.
Initially,
revealed
international
transmission
evolutionary
history
recent
through
phylogenetic
analysis
conducted
using
a
spatiotemporal
Bayesian
framework.
Our
findings
indicate
that
independent
acquisition
via
mobilome,
primarily
plasmids,
transposons,
prophages,
shapes
unique
antimicrobial
resistance
profile
different
lineages.
Notably,
mobilome-resistome
combination
lineages
exhibits
geographical-specific
manner,
further
supporting
localized
endemic
mobilome-driven
process.
Collectively,
this
study
elucidates
adaptation
in
pathogen,
likely
driven
by
farming
style,
provides
valuable
insights
targeted
interventions.
Язык: Английский
Slow-growingSalmonella entericaTyphi mis-identified asSalmonellaGallinarum in Ibadan, Nigeria
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 25, 2024
ABSTRACT
Salmonella
enterica
s
erovar
Typhi
is
endemic
in
Nigeria
where
S.
G
allinarum
rarely
reported.
Probable
S
.
Gallinarum
was
blood-cultured
from
three
Ibadan
patients
within
10-days
leading
to
suspicion
of
an
outbreak.
All
isolates
were
re-identified
using
VITEK-2,
whole
genome
sequenced
on
Illumina
and
Oxford
Nanopore
platforms
confirmed
as
genotype
3.1.1.
Two
the
same
household
had
no
single
nucleotide
polymorphisms,
suggesting
a
point-source,
but
third
independent
infection
event.
The
formed
small
colonies,
negative
for
motility
by
hanging
drop
method,
Sulphide-Indole-Motility
medium
at
24h,
positive
after
48h
incubation.
Hybrid
assemblies
revealed
chromosomal
fragment
arrangements
with
imbalances
either
side
ori
ter
,
which
has
been
shown
slow
growth.
Suspected
typhoid
areas
should
be
evaluated
biochemically
extended
incubation,
verified
serological
or
molecular
methods.
Main
contributions
research
This
study
demonstrates
that
slow-growing
can
mis-identified
resource-limited
countries.
Extended
testing
confirmatory
avoid
miss-calls.
genetic
basis
slow-growth
may
rearrangements
rrn
operons,
are
known
occur
Язык: Английский