Ticks and Tick-borne Diseases, Год журнала: 2019, Номер 10(4), С. 798 - 804
Опубликована: Март 19, 2019
Язык: Английский
Current Biology, Год журнала: 2018, Номер 28(12), С. 1896 - 1902.e5
Опубликована: Июнь 1, 2018
Язык: Английский
Процитировано
287
Parasites & Vectors, Год журнала: 2019, Номер 12(1)
Опубликована: Май 28, 2019
Ticks are obligate blood feeders transmitting major pathogens worldwide. Over the past few years, considerable research efforts have focused on diversity, distribution and impact of gut intracellular bacterial symbionts tick development tick-borne pathogen transmission. The study this internal microbiome requires use a sterilization method to remove external (i.e. cuticular) microbes present tick's surface avoid any further contamination. Several methods exist, including ethanol- or bleach-based treatments that both effective in killing but with different potential effects DNA denaturation.
Язык: Английский
Процитировано
111
Parasites & Vectors, Год журнала: 2020, Номер 13(1)
Опубликована: Янв. 3, 2020
Abstract Background Ticks and tick-borne pathogens (TTBP) are a major constraint to livestock production in Pakistan; despite high prevalence of TTBPs, knowledge on the capacity Pakistani ticks carry endosymbionts is limited. Furthermore, mixed infections with multiple microorganisms further complicate limit detection potential traditional diagnostic methods. The present study investigated bovine Pakistan, employing high-throughput microfluidic real-time PCR based technique. Methods were collected from clinically healthy cattle ( n = 116) water buffaloes 88) 30 villages across six districts located five agro-ecological zones (AEZs) Pakistan September November 2017. was used test genomic DNA individual for presence 27 bacterial eight parasitic microorganisms. Phylogenetic methods assess genetic relationship sequences determined herein. Results detected at least one microorganism each 221 tested (94.4%, 221/234). DNA-based inferred that single pathogens/endosymbionts most common (43.4%, 96/221) followed by double (38.9%, 86/221), triple (14.5%, 32/221), quadruple (2.3%, 5/221) quintuple (0.9%, 2/221) infections. Piroplasms Babesia / Theileria spp.) prevalent (31.6%, 74/234), Ehrlichia spp. (20%, 47/234) Anaplasma marginale (7.7%, 18/234). phagocytophilum , A. ovis centrale Borrelia spp., Rickettsia R. massiliae Bartonella Hepatozoon also detected. Endosymbionts such as Francisella -like (91.5%, 214/234) Coxiella (1.3%, 3/234) organisms ticks. highest diversity Hyalomma anatolicum (test-positive 14/14 microorganisms), Rhipicephalus microplus (4/14), Hy. hussaini (3/14) Rh. annulatus (2/14). carried significantly more frequently piroplasms (41.2%, 54/131; P < 0.05) than those (19.4%, 20/103). However, overall did not vary among two host species well different AEZs. Conclusions To our knowledge, this first investigate wide range using method AEZs Pakistan. These findings will aid establishing distribution patterns control bovines
Язык: Английский
Процитировано
110
Frontiers in Veterinary Science, Год журнала: 2021, Номер 8
Опубликована: Апрель 26, 2021
The zoonosis Q fever is caused by the obligate intracellular bacterium
Язык: Английский
Процитировано
88
Parasite Immunology, Год журнала: 2020, Номер 43(5)
Опубликована: Дек. 13, 2020
The recent development of high-throughput NGS technologies, (ie, next-generation sequencing) has highlighted the complexity tick microbial communities-which include pathogens, symbionts, and commensals-and also their dynamic variability. Symbionts commensals can confer crucial diverse benefits to hosts, playing nutritional roles or affecting fitness, development, nutrition, reproduction, defence against environmental stress immunity. Nonpathogenic bacteria may play a role in modifying tick-borne pathogen colonization transmission, as relationships between microorganisms existing together one environment be competitive, exclusive, facilitating absent, with many potential implications for both human animal health. Consequently, ticks represent compelling yet challenging system which investigate composition functional ecological bacterial communities, thus merits greater attention. Ultimately, deciphering carried by well symbiont-tick interactions will garner invaluable information, aid some future arthropod-pest vector-borne transmission control strategies. This review outlines research on microbiome dynamics, highlights elements favouring reciprocal influence agents finally discusses how diseases might potentially controlled through manipulation future.
Язык: Английский
Процитировано
81
Current Research in Parasitology and Vector-Borne Diseases, Год журнала: 2021, Номер 1, С. 100036 - 100036
Опубликована: Янв. 1, 2021
The main importance of ticks resides in their ability to harbor pathogens that can be transmitted terrestrial vertebrates including humans. Recently, studies have focused on the taxonomic and functional composition tick microbiome, its microbial diversity variation under different factors species, sex, environment among others. Of special interest are interactions between tick, microbiome since influence pathogen colonization within vector, potentially, transmission vertebrate host. In this review, we tackled a synthesis growing field microbiomes. We focus current state research, addressing controversial hotly debated topics advances precise manipulation microbiome. Furthermore, discuss innovative anti-tick microbiota vaccines as possible tool for modulation thus, control tick-borne diseases. Deciphering tick-microbiome spur new strategies diseases via
Язык: Английский
Процитировано
81
Frontiers in Immunology, Год журнала: 2021, Номер 12
Опубликована: Март 2, 2021
Ticks are ectoparasitic arthropods that necessarily feed on the blood of their vertebrate hosts. The success acquisition depends pharmacological properties tick saliva, which is injected into host during feeding. Saliva also used as a vehicle by several types pathogens to be transmitted host, making ticks versatile vectors diseases for humans and other animals. When feeds an infected pathogen reaches gut must migrate its salivary glands via hemolymph successfully subsequent next stage In addition, some can colonize ovaries transovarially progeny. immune system, well system invertebrates, more rudimentary than vertebrates, presenting only innate responses. Although simpler, large number species evidences efficiency system. factors act in each organ interacts with pathogens; therefore, these potential targets development new strategies control tick-borne diseases. objective this review present prevailing knowledge discuss challenges studying immunity, especially regarding gaps interconnections. To end, we use comparative approach focusing various components humoral cellular such signaling pathways, antimicrobial peptides, redox metabolism, complement-like molecules regulated cell death. role microbiota vector competence discussed.
Язык: Английский
Процитировано
80
Frontiers in Microbiology, Год журнала: 2020, Номер 11
Опубликована: Апрель 22, 2020
Obligatory hematophagous arthropods such as lice, bugs, flies, and ticks harbor bacterial endosymbionts that are expected to complement missing essential nutrients in their diet. Genomic some experimental evidence support this expectation. Hard (Acari: Ixodidae) associated with several lineages of symbionts, very few were experimentally shown be aspects tick's fitness. In order pinpoint the nature interactions between hard we tested effect massive elimination Coxiella-like (CLE) by antibiotics on development fitness brown dog tick (Rhipicephalus sanguineus). Administration ofloxacin engorged (blood fed) nymphs resulted significant acute reduction CLE loads - an also persisted subsequent life stages (aposymbiotic ticks). As a result, post-feeding aposymbiotic female (but not male) was delayed. Additionally, adult females needed significantly prolonged feeding period replete (detach from host), had reduced engorgement weight lower capacity produce eggs. Consequently, fecundity fertility reduced. Eggs produced free CLE, resulting larvae unable feed successfully. Our findings demonstrate observed effects due antibiotic administration. suggest contribution is mandatory for oocyte embryogenesis, but required during females, when blood meal processing tissue buildup taking place. Presumably, under these extreme physiological demands, contribute R. sanguineus through supplementing micro- macronutrients. Further nutrient complementary studies hypothesis.
Язык: Английский
Процитировано
76
Nature Reviews Microbiology, Год журнала: 2023, Номер 21(9), С. 604 - 618
Опубликована: Май 22, 2023
Язык: Английский
Процитировано
41
mBio, Год журнала: 2022, Номер 14(1)
Опубликована: Дек. 13, 2022
Insects are highly successful in colonizing a wide spectrum of ecological niches and feeding on diversity diets. This is notably linked to their capacity get from microbiota any essential component lacking the diet such as vitamins amino acids. Over century research based dietary analysis, antimicrobial treatment, gnotobiotic rearing, culture-independent microbe detection progressively generated wealth information about role specific aspects insect fitness. Thanks recent increase sequencing capacities, whole-genome number symbionts has facilitated tracing biosynthesis pathways, validation experimental data evolutionary analyses. field considerable set hosts harboring or nonspecific members. Here, we review current knowledge involvement tick nutrition, with particular focus B vitamin provision. We specifically question if there specificity provision by compared redundant yet contribution microbes. successively highlight known microbial during three main life stages invertebrates: postembryonic development, adulthood, reproduction.
Язык: Английский
Процитировано
39