Drosophila suzukii (Diptera: Drosophilidae): A Decade of Research Towards a Sustainable Integrated Pest Management Program

Journal of Economic Entomology, Год журнала: 2021, Номер 114(5), С. 1950 - 1974

Опубликована: Июль 26, 2021

Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) also known as spotted-wing drosophila (SWD), is a pest native to Southeast Asia. In the last few decades, has expanded its range affect all major European and American fruit production regions. SWD highly adaptive insect that able disperse, survive, flourish under of environmental conditions. Infestation by generates both direct indirect economic impacts through yield losses, shorter shelf life infested fruit, increased costs. Fresh markets, frozen berries, export programs have been impacted due zero tolerance for infestation. As control rely heavily on insecticides, exceedance maximum residue levels (MRLs) resulted in crop rejections. The impact particularly severe organic operations, mainly limited availability effective insecticides. Integrated management (IPM) could significantly reduce chemical inputs but would require substantial changes horticultural practices. This review evaluates most promising methods studied part an IPM strategy against across world. For each considered techniques, effectiveness, impact, sustainability, stage development are discussed.

Язык: Английский

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Highly contiguous assemblies of 101 drosophilid genomes

eLife, Год журнала: 2021, Номер 10

Опубликована: Июль 19, 2021

Over 100 years of studies in Drosophila melanogaster and related species the genus have facilitated key discoveries genetics, genomics, evolution. While high-quality genome assemblies exist for several this group, they only encompass a small fraction genus. Recent advances long-read sequencing allow tens or even hundreds to be efficiently generated. Here, we utilize Oxford Nanopore build an open community resource 101 lines 93 drosophilid encompassing 14 groups 35 sub-groups. The genomes are highly contiguous complete, with average contig N50 10.5 Mb greater than 97% BUSCO completeness 97/101 assemblies. We show that Nanopore-based accurate coding regions, particularly respect insertions deletions. These assemblies, along detailed laboratory protocol assembly pipelines, released as public will serve starting point addressing broad questions ecology, evolution at scale species.

Язык: Английский

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Transposable elements in Drosophila

Mobile DNA, Год журнала: 2020, Номер 11(1)

Опубликована: Июль 3, 2020

has been studied as a biological model for many years and discoveries in biology rely on this species. Research transposable elements (TEs) is not an exception.

Язык: Английский

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f‐Statistics estimation and admixture graph construction with Pool‐Seq or allele count data using the R package poolfstat

Molecular Ecology Resources, Год журнала: 2021, Номер 22(4), С. 1394 - 1416

Опубликована: Ноя. 27, 2021

By capturing various patterns of the structuring genetic variation across populations, f -statistics have proved highly effective for inference demographic history. Such statistics are defined as covariances SNP allele frequency differences among sets populations without requiring haplotype information and hence particularly relevant analysis pooled sequencing (Pool-Seq) data. We here propose a reinterpretation F (and D ) parameters in terms probability gene identity derive from this unified definition unbiased estimators both Pool-Seq data standard count obtained individual genotypes. implemented these new version R package poolfstat, which now includes wide range methods: (i) three-population test admixture; (ii) four-population treeness; (iii) F4 -ratio estimation admixture rates; (iv) fitting, visualization (semi-automatic) construction graphs. A comprehensive evaluation methods poolfstat on simulated (with coverages error rates) confirmed accuracy approaches, even most cost-effective design involving relatively low coverages. further analysed real made 14 invasive species Drosophila suzukii, allowed refining history native invasion routes followed by emblematic pest. Our provides community with user-friendly efficient all-in-one tool to unravel complex population histories large-size or

Язык: Английский

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Behavioral and Genomic Sensory Adaptations Underlying the Pest Activity ofDrosophila suzukii

Molecular Biology and Evolution, Год журнала: 2021, Номер 38(6), С. 2532 - 2546

Опубликована: Фев. 11, 2021

Abstract Studying how novel phenotypes originate and evolve is fundamental to the field of evolutionary biology as it allows us understand organismal diversity generated maintained. However, determining basis challenging involves orchestrated changes at multiple biological levels. Here, we aim overcome this challenge by using a comparative species framework combining behavioral, gene expression, genomic analyses egg-laying substrate-choice behavior invasive pest Drosophila suzukii. First, used behavioral assays evolution ripe fruit oviposition preference in D. suzukii compared with closely related subpulchrella biarmipes well melanogaster. We show that lay eggs on both rotten fruits, suggesting transition was gradual. Second, two-choice assays, studied suzukii, subpulchrella, biarmipes, melanogaster differentially process key sensory cues distinguishing from during egg-laying. found suzukii’s for part mediated through species-specific stiff substrates. Last, sequenced annotated high-quality genome subpulchrella. Using approaches, identified candidate genes involved ability seek out target fruits. Our results provide detail stepwise activity indicating important when finding host, molecular mechanisms potentially underlying their adaptation new ecological niche.

Язык: Английский

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The Worldwide Invasion ofDrosophila suzukiiIs Accompanied by a Large Increase of Transposable Element Load and a Small Number of Putatively Adaptive Insertions

Molecular Biology and Evolution, Год журнала: 2021, Номер 38(10), С. 4252 - 4267

Опубликована: Май 21, 2021

Transposable elements (TEs) are ubiquitous and mobile repeated sequences. They major determinants of host fitness. Here, we characterized the TE content spotted wing fly Drosophila suzukii. Using a recently improved genome assembly, reconstructed sequences de novo found that TEs occupy 47% mostly located in gene-poor regions. The majority insertions segregate at low frequencies, indicating recent probably ongoing activity. To explore dynamics context biological invasions, studied variation abundance genomic data from 16 invasive six native populations D. We large increase load correlated with reduced Watterson estimate genetic diversity θw^ proxy effective population size. did not find any correlation between contents bioclimatic variables, minor effect environmentally induced A genome-wide association study revealed ca. 2,000 regions associated abundance. find, however, evidence such an enrichment for genes known to interact activity (e.g., transcription factor encoding or piRNA pathway). Finally, insertion frequencies 15 putatively adaptive insertions, them being likely invasion history species.

Язык: Английский

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Genomic Designing for Biotic Stress Resistant Grapevine

Springer eBooks, Год журнала: 2022, Номер unknown, С. 87 - 255

Опубликована: Янв. 1, 2022

Язык: Английский

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Population genomics of Drosophila suzukii reveal longitudinal population structure and signals of migrations in and out of the continental United States

G3 Genes Genomes Genetics, Год журнала: 2021, Номер 11(12)

Опубликована: Окт. 2, 2021

Abstract Drosophila suzukii, or spotted-wing drosophila, is now an established pest in many parts of the world, causing significant damage to numerous fruit crop industries. Native East Asia, D. suzukii infestations started United States a decade ago, occupying wide range climates. To better understand invasion ecology this pest, knowledge past migration events, population structure, and genetic diversity needed. In study, we sequenced whole genomes 237 individual flies collected across continental States, as well several sites Europe, Brazil, identify analyze hundreds thousands markers. We observed strong structure between Western Eastern US populations, but no evidence any different latitudes within suggesting that there are broad-scale adaptations occurring response differences winter detect admixture from Hawaii agreement with previously identified introduction routes inferred microsatellite analysis. also potential signals back which could have important implications for shipping quarantine policies exported agriculture. anticipate large genomic dataset will spur future research into underlying activity development novel control methods agricultural pest.

Язык: Английский

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Higher evolutionary dynamics of gene copy number for Drosophila glue genes located near short repeat sequences

BMC Ecology and Evolution, Год журнала: 2024, Номер 24(1)

Опубликована: Фев. 2, 2024

Abstract Background During evolution, genes can experience duplications, losses, inversions and gene conversions. Why certain are more dynamic than others is poorly understood. Here we examine how several Sgs encoding glue proteins, which make up a bioadhesive that sticks the animal during metamorphosis, have evolved in Drosophila species. Results We examined high-quality genome assemblies of 24 species to study evolutionary dynamics four present D. melanogaster part same family - Sgs1, Sgs3, Sgs7 Sgs8 across approximately 30 millions years. annotated total 102 grouped them into 4 subfamilies. here new nomenclature for these based on protein sequence conservation, genomic location presence/absence internal repeats. Two types were uncovered. The first category ( Sgs3x, Sgs3e ) showed few losses but no duplication, local inversion conversion. second group Sgs3b, Sgs7, exhibited multiple events Our data suggest presence short “new glue” near latter may accelerated their dynamics. Conclusions comparative analysis suggests influenced by context. molecular, phylogenetic provides foundation investigating role various life.

Язык: Английский

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Biotechnology-enhanced genetic controls of the global pest Drosophila suzukii

Trends in biotechnology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

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