Inhibitory effects of luteolin on TLR3-mediated inflammation caused by TAK/NF-κB signaling in human corneal fibroblasts

International Journal of Ophthalmology, Год журнала: 2022, Номер 15(3), С. 371 - 379

Опубликована: Март 2, 2022

To study the role of luteolin (LUT) in expression toll-like receptors 3 (TLR3) ligand polyI:C stimulated inflammatory factors human corneal fibroblasts (HCFs).HCFs cells were cultivated with or without LUT polyI:C. The levels interleukin (IL)-6, IL-8, monocyte chemotactic protein-1 (MCP-1), vascular cell adhesion molecule (VCAM)-1, as well intercellular (ICAM)-1 measured using enzyme-linked immunosorbent assay (ELISA), immunoblotting reverse transcription-quantitative polymerase chain reaction (PCR) analyses. Immunoblotting was used to assess toll-interleukin-1 receptor-domain-containing adapter-inducing interferon-β (TRIF), TLR3, transforming growth factor-b-activated kinase 1 (TAK1), tumor necrosis factor receptor-associated 6 (TRAF6), transcription AP-1, nuclear (NF-κB)-inhibitory protein IκB-α degradation and phosphorylation. Immunofluorescence assays localize cellular location p65 subunit NF-κB.Corneal exposed demonstrated decreased VCAM-1, ICAM-1, MCP-1, IL-6, IL-8 upon exposure a time-dependent concentration-dependent manner. observed suppress polyI:C-triggered translocation NF-κB into nuclei, phosphorylation TAK, c-Jun, IκB-α, while no impact on TRIF TRAF6 observed.LUT proinflammatory molecules, chemokines, cytokines HCFs. These effects are likely mediated through TAK/NF-κB signal attenuation. Therefore, is candidate that can prevent TLR3-mediated inflammation response associated viral infection.

Язык: Английский

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Biomaterials used for tissue engineering of barrier-forming cell monolayers in the eye

Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11

Опубликована: Сен. 27, 2023

Cell monolayers that form a barrier between two structures play an important role for the maintenance of tissue functionality. In anterior portion eye, corneal endothelium forms controls fluid exchange aqueous humor chamber and stroma. This monolayer is central in pathogenesis Fuchs endothelial dystrophy (FECD). FECD common disease, which cells deposit extracellular matrix increases thickness its basal membrane (Descemet’s membrane), excrescences (guttae). With time, there decrease cell density generates vision loss. Transplantation healthy on Descemet substitute could become interesting alternative treatment this pathology. back retinal pigment epithelium (RPE) blood-retinal barrier, controlling choriocapillaris photoreceptors outer retina. disease dry age-related macular degeneration (dry AMD), deposits (drusen) RPE (Bruch’s membrane). These hinder exchange, resulting progressive death, turn photoreceptor Bruch’s membrane/choroidal stromal to replace before death eye disease. review will present different biomaterials are proposed engineering FECD, AMD.

Язык: Английский

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The Potential Reversible Transition between Stem Cells and Transient-Amplifying Cells: The Limbal Epithelial Stem Cell Perspective

Cells, Год журнала: 2024, Номер 13(9), С. 748 - 748

Опубликована: Апрель 25, 2024

Stem cells (SCs) undergo asymmetric division, producing transit-amplifying (TACs) with increased proliferative potential that move into tissues and ultimately differentiate a specialized cell type. Thus, TACs represent an intermediary state between stem differentiated cells. In the cornea, population of resides in limbal region, named epithelial (LESCs). As LESCs proliferate, they generate centripetally cornea corneal Upon injury, research suggests progenitor-like exists within can centrifugally limbus, where dedifferentiate LESCs. Herein, we summarize recent advances made understanding mechanism governs differentiation TACs, thereafter, We also outline evidence support existence whether could capabilities cornea. Furthermore, to gain further insights dynamics most findings other organ systems hypothesis SCs.

Язык: Английский

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Decoding cellular plasticity and niche regulation of limbal stem cells during corneal wound healing

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Июль 6, 2024

Abstract Background Dysfunction or deficiency of corneal epithelium results in vision impairment blindness severe cases. The rapid and effective regeneration epithelial cells relies on the limbal stem (LSCs). However, molecular functional responses LSCs their niche to injury remain elusive. Methods Single-cell RNA sequencing was performed tissues from normal mice defect models. Bioinformatics analysis confirm distinct characteristics cell fates LSCs. Knockdown Creb5 OSM treatment experiment were determine roles wound healing. Results Our data defined signatures reconstructed pseudotime trajectory cells. Gene network analyses characterized transcriptional landmarks that potentially regulate LSC dynamics, identified a transcription factor Creb5, expressed significantly upregulated after injury. Loss-of-function experiments revealed silencing delayed healing mobilization. Through cell–cell communication analysis, we 609 candidate regeneration-associated ligand-receptor interaction pairs between cells, discovered unique subset Arg1 + macrophages infiltrated injury, which present as source Oncostatin M (OSM), an IL-6 family cytokine, demonstrated effectively accelerate Conclusions This research provides valuable single-cell resource reference for discovery mechanisms potential clinical interventions aimed at ocular surface reconstruction.

Язык: Английский

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Biology and medicine on ocular surface restoration: advancements and limits of limbal stem cell deficiency treatments

The Ocular Surface, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

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