Conserved, yet disruption-prone, gut microbiomes in neotropical bumblebees
mSphere,
Год журнала:
2023,
Номер
8(6)
Опубликована: Окт. 19, 2023
Social
bees
are
an
important
model
for
the
ecology
and
evolution
of
gut
microbiomes.
These
harbor
ancient,
specific,
beneficial
microbiomes
crucial
pollinators.
However,
most
research
has
concentrated
on
managed
honeybees
bumblebees
in
temperate
zone.
Here
we
used
16S
rRNA
gene
sequencing
to
characterize
wild
neotropical
bumblebee
communities
from
Colombia.
We
also
analyzed
drivers
microbiome
structure
across
our
data
previously
published
bumblebees.
Our
results
show
that
lineages
not
only
retained
their
ancient
bacterial
symbionts
during
dispersal
North
America
but
prone
major
disruption,
a
shift
is
strongly
associated
with
parasite
infection.
Finally,
found
much
more
structured
by
host
phylogeny
than
geography,
despite
very
different
environmental
conditions
plant
two
regions.
Язык: Английский
The trypanosomatid (Kinetoplastida: Trypanosomatidae) parasites in bees: A Review on their environmental circulation, impacts and implications
Current Research in Insect Science,
Год журнала:
2025,
Номер
7, С. 100106 - 100106
Опубликована: Янв. 1, 2025
Язык: Английский
Bumble bee gut microbial community structure differs between species and commercial suppliers, but metabolic potential remains largely consistent
Applied and Environmental Microbiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 6, 2025
ABSTRACT
Bumble
bees
are
key
pollinators
for
natural
and
agricultural
plant
communities.
Their
health
performance
supported
by
a
core
gut
microbiota
composed
of
few
bacterial
taxa.
However,
the
taxonomic
composition
community
structure
bumble
bee
microbiotas
can
vary
with
species,
environment,
origin
(i.e.,
whether
colonies
come
from
wild
or
commercial
rearing
facility),
it
is
unclear
metabolic
capabilities
therefore
as
well.
Here
we
used
metagenomic
sequencing
to
examine
composition,
structure,
potential
across
two
different
Bombus
impatiens
suppliers,
B.
,
three
other
species
sampled
sites
within
native
range
all
four
species.
We
found
that
varied
between
populations
origins
suppliers.
Notably,
Apibacter
consistently
present
in
some
species—suggesting
may
be
previously
unrecognized
phylotype
bees—and
lack
phylotypes
populations.
despite
variation
high-level
was
largely
consistent
hosts,
including
related
host
performance,
though
activity
remains
investigated.
IMPORTANCE
Our
study
first
compare
genome-level
whole
suppliers
In
addition,
profiled
full
time.
Overall,
our
results
provide
new
insight
into
function
will
help
researchers
evaluate
how
well
studies
conducted
one
population
translate
Research
on
increasing
relevance
continue
discover
ways
social
influence
health,
decline
abundance.
Язык: Английский
Changes in bumblebee queen gut microbiotas during and after overwintering diapause
Insect Molecular Biology,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 22, 2024
Abstract
Bumblebees
are
key
pollinators
with
gut
microbiotas
that
support
host
health.
After
bumblebee
queens
undergo
winter
diapause,
which
occurs
before
spring
colony
establishment,
their
disturbed,
but
little
is
known
about
community
dynamics
during
diapause
itself.
Queen
also
help
seed
worker
microbiotas,
so
it
important
they
recover
post‐diapause
to
a
typical
structure,
process
may
be
impeded
by
pesticide
exposure.
We
examined
how
queen
microbiota
structure
and
metabolic
potential
shift
after
whether
recovery
affected
To
do
so,
we
placed
commercial
Bombus
impatiens
into
euthanizing
them
at
0,
2
4
months
of
diapause.
Additionally,
allowed
some
from
for
1
week
euthanasia,
exposing
half
the
common
herbicide
glyphosate.
Using
whole‐community,
shotgun
metagenomic
sequencing,
found
core
bee
phylotypes
dominated
before,
two
phylotypes,
Schmidhempelia
Snodgrassella
,
ceased
detected
late
recovery.
Despite
fluctuations
in
taxonomic
remained
constant
through
Also,
glyphosate
exposure
did
not
affect
However,
assembly
quality
our
ability
detect
microbial
taxa
pathways
declined
alongside
abundance,
was
substantially
reduced
Our
study
offers
new
insights
change
taxonomically
functionally
life
stage
provides
guidance
future
studies
diapausing
bumblebees.
Язык: Английский
Neither sulfoxaflor, Crithidia bombi, nor their combination impact bumble bee colony development or field bean pollination
Scientific Reports,
Год журнала:
2023,
Номер
13(1)
Опубликована: Сен. 30, 2023
Many
pollinators,
including
bumble
bees,
are
in
decline.
Such
declines
known
to
be
driven
by
a
number
of
interacting
factors.
Decreases
bee
populations
may
also
negatively
impact
the
key
ecosystem
service,
pollination,
that
they
provide.
Pesticides
and
parasites
often
cited
as
two
drivers
declines,
particularly
have
previously
been
found
interact
with
one
another
detriment
health.
Here
we
test
effects
an
insecticide,
sulfoxaflor,
highly
prevalent
parasite,
Crithidia
bombi,
on
Bombus
terrestris.
After
exposing
colonies
realistic
doses
either
sulfoxaflor
and/or
bombi
fully
crossed
experiment,
were
allowed
forage
field
beans
outdoor
exclusion
cages.
Foraging
performance
was
monitored,
impacts
fruit
set
recorded.
We
no
effect
stressor,
or
their
interaction,
pollination
services
provide
beans,
at
individual
level
whole
colony
level.
Further,
there
any
treatment,
metric,
development.
Our
results
contrast
prior
findings
similar
insecticides
(neonicotinoids)
services,
development,
potentially
suggesting
is
less
harmful
compound
health
than
neonicotinoids
insecticides.
Язык: Английский
Glyphosate Effects on Growth and Biofilm Formation in Bee Gut Symbionts and Diverse Associated Bacteria
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 21, 2024
Abstract
Biofilm
formation
is
a
common
adaptation
enabling
bacteria
to
thrive
in
various
environments
and
withstand
external
pressures.
In
the
context
of
host-microbe
interactions,
biofilms
play
vital
roles
establishing
microbiomes
associated
with
animals
plants
are
used
by
opportunistic
microbes
facilitate
proliferation
within
hosts.
Investigating
biofilm
dynamics,
composition,
responses
environmental
stressors
crucial
for
understanding
microbial
community
assembly
regulation
health
disease.
this
study,
we
explore
independent
gut
colonization
vitro
abilities
core
members
honey
bee
(
Apis
mellifera
)
microbiota.
Additionally,
assess
impact
glyphosate,
widely
herbicide
antimicrobial
properties,
glyphosate-based
formulation
on
growth
symbionts
as
well
other
biofilm-forming
diverse
plants.
Our
results
demonstrate
that
several
strains
bacterial
species
can
independently
colonize
gut,
which
probably
depends
their
ability
form
biofilms.
Furthermore,
glyphosate
exposure
has
varying
effects
formation.
These
findings
imply
specific
impacts
both
ecological
host
health-related
implications.
Importance
Biofilms
essential
communities
establish
environments.
microbiota
member
Snodgrassella
alvi
forms
biofilms,
potentially
aiding
establishment
promoting
interactions
host.
show
members,
including
Bifidobacterium
,
Bombilactobacillus
Gilliamella
Lactobacillus
also
We
then
examine
disrupts
microbiota,
formation,
ranging
from
inhibition
enhancement,
reflecting
observations
beneficial
or
pathogenic
Thus,
may
influence
shaping
surfaces
impacting
outcomes.
Язык: Английский
Glyphosate effects on growth and biofilm formation in bee gut symbionts and diverse associated bacteria
Applied and Environmental Microbiology,
Год журнала:
2024,
Номер
90(8)
Опубликована: Авг. 21, 2024
Biofilm
formation
is
a
common
adaptation
enabling
bacteria
to
thrive
in
various
environments
and
withstand
external
pressures.
In
the
context
of
host-microbe
interactions,
biofilms
play
vital
roles
establishing
microbiomes
associated
with
animals
plants
are
used
by
opportunistic
microbes
facilitate
survival
within
hosts.
Investigating
biofilm
dynamics,
composition,
responses
environmental
stressors
crucial
for
understanding
microbial
community
assembly
regulation
health
disease.
this
study,
we
explore
Язык: Английский
Bioaccumulation of polycyclic aromatic hydrocarbons and microbiota dynamics across developmental stages of the Asian tiger mosquito, Aedes albopictus exposed to urban pollutants
Ecotoxicology and Environmental Safety,
Год журнала:
2024,
Номер
286, С. 117214 - 117214
Опубликована: Окт. 23, 2024
Язык: Английский
Seasonally increasing parasite load is associated with microbiome dysbiosis in wild bumblebees
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 1, 2023
Abstract
The
microbiome
is
increasingly
recognized
for
its
complex
relationship
with
host
fitness.
Bumblebees
are
to
a
characteristic
gut
community
that
derived
and
reinforced
through
social
contact
between
individuals.
bumblebee
species-poor,
primarily
composed
from
small
number
of
core
taxa
associated
the
greater
tribe
corbiculate
bees.
Experimental
findings
support
role
in
resistance
severe
infections
by
common
trypanosomal
parasite,
Crithidia
bombi
.
However,
most
studies
have
been
scale,
often
considering
just
one
or
two
species,
making
use
commercially-reared
To
better
understand
diversity
wild
populations,
we
deeply
sampled
field
populations
ten
sympatric
species
found
throughout
central
down
east
Maine
three-year
survey.
We
used
16S
amplicon
sequencing
produce
profiles,
qPCR
screen
samples
breadth
our
dataset
has
enabled
us
test
seasonal
interspecific
trends
community.
Controlling
these
external
sources
variation,
identified
microbial
factors
infection
parasite
load
infection.
Язык: Английский
A case study of the diet-microbiota-parasite interplay in bumble bees
Journal of Applied Microbiology,
Год журнала:
2023,
Номер
134(12)
Опубликована: Дек. 1, 2023
Diets
and
parasites
influence
the
gut
bacterial
symbionts
of
bumble
bees,
but
potential
interactive
effects
remain
overlooked.
The
main
objective
this
study
was
to
assess
isolated
sunflower
pollen,
its
phenolamides,
widespread
trypanosomatid
Crithidia
sp.
on
Bombus
terrestris
males.Bumble
bee
males
emerged
in
microcolonies
fed
either
(i)
willow
pollen
(control),
(ii)
or
(iii)
spiked
with
phenolamide
extracts
from
pollen.
These
were
infected
by
pathogen-free.
Using
16S
rRNA
amplicon
sequencing
(V3-V4
region),
we
observed
a
significant
alteration
beta
diversity
not
alpha
microbial
communities
compared
control
Similarly,
infection
parasite
altered
males,
irrespective
diet.
By
contrast,
did
observe
any
phenolamide-enriched
Changes
indicate
dissimilarities
taxa
between
treatment
groups,
while
lack
difference
demonstrates
no
changes
within
each
group.Bumble
bees
harbour
consistent
microbiota
worldwide,
our
results
suggest
that
are
somewhat
shaped
their
diets
as
well
interaction
these
two
factors.
This
confirms
suitable
biological
surrogates
effect
diet
infections
communities.
Язык: Английский