Type VI secretion systems promote intraspecific competition and host interactions in a bee gut symbiont DOI Creative Commons
Erick V. S. Motta, Patrick J. Lariviere, Korin Rex Jones

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(44)

Опубликована: Окт. 23, 2024

The Type VI Secretion System (T6SS) is a sophisticated mechanism utilized by gram-negative bacteria to deliver toxic effector proteins into target cells, influencing microbial community dynamics and host interactions. In this study, we investigated the role of T6SSs in Snodgrassella alvi wkB2, core bacterial symbiont honey bee gut microbiota. We generated single- double-knockout mutants targeting essential genes ( tssD tssE ) both T6SS-1 T6SS-2 assessed their colonization competition capabilities vivo. Our results indicate that are nonessential for gut, although mutant strains exhibited significantly lower levels compared wild-type (WT) strain. Further, defined experiment showed S. wkB2 do not impact interspecific among bacteria. However, cocolonization experiments with closely related demonstrated plays mediating intraspecific competition. Transcriptomic analysis guts monocolonized WT or T6SS revealed differential expression immunity-related relative microbiota-deprived bees, such as upregulation antimicrobial peptide apidaecin presence defensin , suggesting contribute shaping immune responses. These findings provide insight ecological roles microbiota, emphasizing importance maintaining competitive host–bacterial

Язык: Английский

Type VI secretion systems promote intraspecific competition and host interactions in a bee gut symbiont DOI Creative Commons
Erick V. S. Motta, Patrick J. Lariviere, Korin Rex Jones

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(44)

Опубликована: Окт. 23, 2024

The Type VI Secretion System (T6SS) is a sophisticated mechanism utilized by gram-negative bacteria to deliver toxic effector proteins into target cells, influencing microbial community dynamics and host interactions. In this study, we investigated the role of T6SSs in Snodgrassella alvi wkB2, core bacterial symbiont honey bee gut microbiota. We generated single- double-knockout mutants targeting essential genes ( tssD tssE ) both T6SS-1 T6SS-2 assessed their colonization competition capabilities vivo. Our results indicate that are nonessential for gut, although mutant strains exhibited significantly lower levels compared wild-type (WT) strain. Further, defined experiment showed S. wkB2 do not impact interspecific among bacteria. However, cocolonization experiments with closely related demonstrated plays mediating intraspecific competition. Transcriptomic analysis guts monocolonized WT or T6SS revealed differential expression immunity-related relative microbiota-deprived bees, such as upregulation antimicrobial peptide apidaecin presence defensin , suggesting contribute shaping immune responses. These findings provide insight ecological roles microbiota, emphasizing importance maintaining competitive host–bacterial

Язык: Английский

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