Experimental and Pre-Analytical Considerations of Endocannabinoid Quantification in Human Biofluids Prior to Mass Spectrometric Analysis

Targets, Год журнала: 2025, Номер 3(1), С. 11 - 11

Опубликована: Март 11, 2025

The quantification of endocannabinoids in biological fluids is becoming increasingly popular as an indicator psychological and physiological function. Numerous methods to quantify the endocannabinoid ligands have been published so far, yet their concentrations responses often exhibit significant variability across studies. Endocannabinoids regulate interact with a wide range biomolecules, causing vary between cohorts individuals, sensitivities them depend on pre-experimental behaviours activities. Moreover, matrix effects produced by complex nature biofluids necessitate rigorous sample preparation techniques, all which introduce opportunities for both inter- intra-assay variability. This review aims address causes prior mass spectrometric analysis, including biofluid choice, human variability, collection extraction methods. If these factors are fully considered standardised introduced, may become more reliable, allowing utility clinical markers progress.

Язык: Английский

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Advances in targeted liquid chromatography‐tandem mass spectrometry methods for endocannabinoid and N‐acylethanolamine quantification in biological matrices: A systematic review

Mass Spectrometry Reviews, Год журнала: 2024, Номер unknown

Опубликована: Июль 3, 2024

Liquid chromatography paired with tandem mass spectrometry (LC-MS/MS) is the gold standard in measurement of endocannabinoid concentrations biomatrices. We conducted a systematic review literature to identify advances targeted LC-MS/MS methods period 2017-2024. found that for quantification are relatively consistent both across time and Recent have primarily been three areas: (1) sample preparation techniques, specific chosen biomatrix; (2) range biomatrices tested, recently favoring blood matrices; (3) breadth endocannabinoid-like analytes incorporated into assays. This provides summary recent guide researchers looking establish best quantifying endocannabinoids

Язык: Английский

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Peripheral endocannabinoids in major depressive disorder and alcohol use disorder: a systematic review

BMC Psychiatry, Год журнала: 2024, Номер 24(1)

Опубликована: Авг. 8, 2024

Abstract Background Major Depressive Disorder (MDD) and Alcohol Use (AUD) are two high-prevalent conditions where the Endocannabinoid system (ECS) is believed to play an important role. The ECS regulates how different neurotransmitters interact in both disorders, which crucial for controlling emotions responses stress reward stimuli. Measuring peripheral endocannabinoids (eCBs) human serum plasma can help overcome limitations of detecting endocannabinoid levels brain. This systematic review aims identify eCBs patients with MDD and/or AUD find use as diagnostic, prognostic biomarkers, potential therapeutic targets. Methods We conducted a literature search according Preferred Reporting Items Systematic Reviews Meta-Analysis (PRISMA) guidelines from earliest manuscript until October 22, 2023, three electronic databases. included studies adults who had current diagnosis evaluated or endocannabinoids. carefully considered known variables that may affect levels. Results 17 articles this review, measured 170 359 patients. Stressors increase 2-arachidonyl-glycerol (2-AG) concentrations, 2-AG be particular feature depression severity chronicity. Anxiety symptoms negatively correlated anandamide (AEA) AEA significantly increases during early abstinence AUD. Studies suggest negative correlation between Oleoylethanolamide (OEA) length They also show significant OEA fatty acid amide hydrolase (FAAH) activity. Eicosapentaenoylethanolamide (EPEA) clinical remission rates depression. Included such gender, chronicity, symptom severity, comorbid psychiatric symptoms, case AUD, stress-inducibility eCBs. Conclusions highlights role plays Peripheral appear useful biomarkers these further research Using accessible biological samples blood well-designed develop novel therapies disorders.

Язык: Английский

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Sex Differences in Endocannabinoid and Inflammatory Markers Associated with Posttraumatic Stress Disorder

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 14, 2025

Abstract Background Posttraumatic stress disorder (PTSD) is one of the most sex-polarized psychiatric disorders, with women exhibiting twice prevalence men. The biological mechanisms underlying this sex disparity are not fully understood. Growing evidence suggests that alterations stress-buffering endocannabinoid (eCB) system and heightened inflammation central to PTSD pathophysiology may contribute sex-biases in risk severity. Here, we examined sex-differences levels circulating eCBs peripheral pro-inflammatory markers a cohort men non-psychiatric controls. Methods 88 individuals 85 sex- age-matched controls (HC) were retrospectively selected from Mass General Brigham Biobank. Serum was assayed measure [anandamide (AEA), 2-arachidonyl glycerol (2-AG), oleoylethanolamide (OEA), arachidonic acid (AA] inflammatory [interleukin-1β (IL-1β), IL-6, IL-8, IL-18, tumor necrosis factor-alpha (TNFα), C-reactive Protein (CRP)]. Linear regression used predict differential abundance each analyte by disease state (PTSD/HC) within male female subgroups. Two-sided t-tests Benjamini-Hochberg correction examine differences across Analyses repeated those comorbid major depressive disorder. Results Male patients showed significant decrease AEA, AA OEA compared ( p ’s < .001) subgroups (PTSD HCs) p< .001 ). In contrast, among females, elevated IL-6 p<.05 ) IL-8 Both exhibited an increase TNFα concentrations ), HCs. Similar results obtained subgroup MDD after controlling for FAAH 385A genotype. Conclusions Our findings show first time eCB absent PTSD, who turn exhibit broader markers, thus suggesting perturbations vary sex.

Язык: Английский

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Endocannabinoid system modulation for visceral abdominal pain in inflammatory bowel disease and irritable bowel syndrome: A protocol for systematic review and meta-analysis

HRB Open Research, Год журнала: 2025, Номер 8, С. 40 - 40

Опубликована: Март 13, 2025

Visceral Pain is a common debilitating symptom of inflammatory bowel disease (IBD) and irritable syndrome (IBS). The endocannabinoid system (ECS) prime target for alleviation visceral pain, given its important role in both gastrointestinal physiology pain. We will conduct systematic review randomised controlled trials (RCTs) cannabis, cannabinoids, cannabis-based medicines (CBMs), other ECS modulators patients with IBD IBS, comparing any preparation cannabinoid, CBM, or pharmacological modulator the (in dose, by route administration), control (placebo, / psychological dietary intervention). search CENTRAL, MEDLINE (PubMed), EMBASE, Web Science databases, as well WHO International Clinical Trials Registry Platform ClinicalTrials.gov Registries, together reference checking citation searching, following PRISMA guidelines. Our objectives are to evaluate benefits harms modulation abdominal pain compared placebo interventions. primary outcomes be proportion people (a) at least 30% reduction (b) 50% intensity defined IMMPACT. Secondary include change intensity, physical emotional functioning, fatigue sleep measures, quality life, severity, adverse effects. assess risk bias RCTs using Cochrane Risk Bias 2 tool. Where there sufficient data that directly comparable, we meta-analyses results each outcome. use GRADEpro GDT tool certainty evidence This synthesise available regarding all types treatment related comorbidities IBS patients.

Язык: Английский

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Exploratory study on plasma Acylglycerol and Acylethanolamide dysregulation in substance use and attention-deficit/hyperactivity disorder: Implications for novel biomarkers in dual diagnosis

Progress in Neuro-Psychopharmacology and Biological Psychiatry, Год журнала: 2025, Номер unknown, С. 111350 - 111350

Опубликована: Апрель 1, 2025

Substance use disorder (SUD) is a major global public health challenge, frequently co-occurring with psychiatric conditions such as attention-deficit/hyperactivity (ADHD). Endocannabinoid system (ECS) dysregulation has been implicated in both SUD and ADHD, but the interplay between these remains poorly understood. This study investigates plasma concentrations of endocannabinoid-congeners individuals SUD, without comorbid to identify potential biomarkers. exploratory included 469 participants divided into three groups: (1) healthy controls (n = 136), (2) patients ADHD 267), (3) 66). Plasma 12 endocannabinoid-related molecules, including acylglycerols (2-AG, 2-LG, 2-OG) acylethanolamides (AEA, DEA, DHEA, DGLEA, LEA, OEA, PEA, POEA, SEA), were quantified using high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS). A multinomial Elastic Net regression model was applied assess their biomarker potential. Patients exhibited significantly lower 2-AG 2-LG compared controls, while most elevated, except for POEA SEA. comorbidity associated 2-AG, AEA, SEA, PEA elevated. Machine learning analysis identified SEA key biomarkers, achieving an accuracy 72.1 % ROC-AUC 0.77. suggests distinct ECS alterations highlighting Future research should validate findings larger cohorts explore ECS-targeted therapeutic interventions dual-diagnosis populations.

Язык: Английский

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Validation of a Lc-Ms/Ms Method for Quantification of Testosterone, Allopregnanolone and its Isomers in Human Serum: Comparison to Immunoassay

Опубликована: Янв. 1, 2024

Язык: Английский

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Sex-Specific Changes to Brain Fatty Acids, Plasmalogen, and Plasma Endocannabinoids in Offspring Exposed to Maternal and Postnatal High-Linoleic-Acid Diets

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(14), С. 7911 - 7911

Опубликована: Июль 19, 2024

Linoleic acid (LA) is required for neuronal development. We have previously demonstrated sex-specific changes in cardiovascular and hepatic function rat offspring from mothers consuming a high-LA diet, with some effects associated reduced LA concentration the postnatal diet. At this time, impact of high-maternal-LA diet on brain development potential to alter any adverse are unknown. Rat fed low- (LLA) or (HLA) diets during pregnancy lactation were weaned at day 25 (PN25) LLA HLA until sacrifice adulthood (PN180). In offspring’s brains, increased docosapentaenoate males. The maternal LA, arachidonate, docosapentaenoate, C18:0 dimethylacetal (DMA), C16:0 DMA, DMA/C16:0, DMA/C18:0, but decreased eoicosenoate, nervoniate, lignocerate, oleate Maternal vaccenate had an interaction effect myristate, palmitoleate, eicosapentaenoate females, eicosadienoate. Postnatal stearate docosapentaenoate. oleate, arachidate, docosahexaenoic (DHA)/omega (n)-6 docosapentaenoic (DPA) females. DHA/n-6 DPA males plasma endocannabinoids (arachidonoyl ethanolamide 2-arachidonoyl glycerol), as well other N-acyl ethanolamides testosterone. alters fatty acids, endocannabinoids, plasmalogen concentrations development-specific manner.

Язык: Английский

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Age and sex effects of a validated LC-MS/MS method for the simultaneous quantification of testosterone, allopregnanolone, and its isomers in human serum

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 13, 2024

Abstract Despite the great relevance of neurosteroid allopregnanolone and related isomers to various health conditions, quantification typically involves immunoassay, which suffers from serious issues with cross-reactivity closely molecules. This article describes development partial validation a liquid chromatography coupled tandem mass spectrometry assay for simultaneous allopregnanolone, pregnanolone, isopregnanolone, epi-allopregnanolone, testosterone in human serum healthy males females aged 5–85 years. 1-amino-4-methylpiperazine (AMP) was used as derivatisation reagent enhance ionisation signal. Linearity calculated at 0.99 lower limit 10.08 pg/mL along linearity 0.98 42.32 testosterone. Application method showed sex age effects across lifespan both testosterone, whereas comparative immunoassay not able detect differences same samples. Our this should provide new tool researchers discover role its health, how it compares hormones.

Язык: Английский

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Maternal obesity changes the small intestine endocannabinoid system and fecal metabolites of weanling rats associated with reduced intestinal permeability and impaired glucose homeostasis

The Journal of Nutritional Biochemistry, Год журнала: 2024, Номер 136, С. 109802 - 109802

Опубликована: Ноя. 14, 2024

Язык: Английский

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