Homeostatic artificial cells enable self-protection in prototissue spheroids
Chem,
Год журнала:
2025,
Номер
unknown, С. 102409 - 102409
Опубликована: Фев. 1, 2025
The
bigger
pictureLiving
cells
form
tissues
with
specialized
functions
that
maintain
homeostasis
through
feedback-regulated
operations.
In
tissues,
cellular
communication
and
collective
behavior
are
crucial
for
coordinating
tasks
ensuring
overall
functionality.
Developing
artificial
(ACs)
can
be
organized
into
prototissues
to
mimic
these
behaviors
is
a
significant
advancement
toward
creating
functional
AC
systems.
These
systems
essential
understanding
how
design
networks
behaviors,
deciphering
life's
fundamental
principles,
advancing
biomedical
applications.
Here,
we
create
AC-based
prototissue
spheroids
stabilize
their
microenvironment
against
external
pH
threats
using
mechanism
encoded
within
individual
ACs.
Like
living
communicate
chemically
collectively
resist
environmental
challenges,
paving
the
way
more
advanced
in
future.Highlights•Microfluidic
production
of
reversible
permeability•Homeostatic
ACs
modulate
internal
via
chemo-structural
feedback•Prototissues
states
threats•Inter-AC
mixed
counteracts
threatsSummaryPrototissues
made
from
aim
replicate
such
as
communication,
behavior,
homeostasis.
Despite
progress
developing
diverse
types,
building
achieving
effective
well
remain
challenging.
We
introduce
an
intrinsic
homeostatic
control
stable
protect
cargo
fluctuations.
contain
pH-modulating
enzymes
pH-sensitive
membrane,
allowing
self-regulation
feedback.
They
adjust
by
importing
substrates
specific
range
self-regulate
substrate
influx.
This
enables
them
local
pH,
manage
release,
facilitate
interactive
spheroids.
Our
findings
demonstrate
potential
synthetic
tissue
mimics,
replicating
protective
communicative
engineering
applications.Graphical
abstract
Язык: Английский
Protein Recruitment to Dynamic DNA-RNA Host Condensates
Journal of the American Chemical Society,
Год журнала:
2024,
Номер
146(43), С. 29344 - 29354
Опубликована: Окт. 17, 2024
We
describe
the
design
and
characterization
of
artificial
nucleic
acid
condensates
that
are
engineered
to
recruit
locally
concentrate
proteins
interest
in
vitro.
These
emerge
from
programmed
interactions
nanostructured
motifs
assembling
three
DNA
strands
one
RNA
strand
can
include
an
aptamer
domain
for
recruitment
a
target
protein.
Because
designed
form
regardless
presence
protein,
they
function
as
"host"
compartments.
As
model
we
consider
Streptavidin
(SA)
due
its
widespread
use
binding
assays.
In
addition
demonstrating
protein
recruitment,
two
approaches
control
onset
condensation
recruitment.
The
first
approach
uses
UV
irradiation,
physical
stimulus
bypasses
need
exchanging
molecular
inputs
is
particularly
convenient
emulsion
droplets.
second
transcription,
ubiquitous
biochemical
reaction
central
development
next
generation
living
materials.
then
show
combination
transcription
degradation
leads
autonomous
dissipative
system
which
host
occur
transiently
condensate
size
well
time
scale
transition
be
controlled
by
level
RNA-degrading
enzyme.
conclude
biotinylated
beads
recruited
SA-host
condensates,
may
therefore
find
immediate
separation
variety
biotin-tagged
components.
Язык: Английский
Coacervate Droplets as Biomimetic Models for Designing Cell‐Like Microreactors
Macromolecular Rapid Communications,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 26, 2024
Abstract
Coacervates
are
versatile
compartments
formed
by
liquid–liquid
phase
separation.
Their
dynamic
behavior
and
molecularly
crowded
microenvironment
make
them
ideal
materials
for
creating
cell‐like
systems
such
as
synthetic
cells
microreactors.
Recently,
combinations
of
natural
molecules
have
been
exploited
via
simple
or
complex
coacervation
to
create
that
can
be
used
build
hierarchical
chemical
with
life‐like
properties.
This
review
highlights
recent
advances
in
the
design
coacervate
their
application
biomimetic
reactors
cell
mimicking
systems.
It
first
explores
variety
influence
structure
on
controlled
behavior.
Then,
applications
coacervates
reviewed,
focusing
how
they
microreactors
through
ability
sequester
provide
a
distinct
regulatory
reactions
aqueous
media.
Язык: Английский
Coacervation in systems chemistry
Communications Chemistry,
Год журнала:
2024,
Номер
7(1)
Опубликована: Ноя. 22, 2024
Communications
Chemistry
is
delighted
to
introduce
a
Collection
of
articles
that
combine
coacervation
with
systems
chemistry.
Here,
the
Guest
Editors
importance
research
at
interface
between
these
two
topics
and
highlight
current
challenges
in
this
area.
Язык: Английский
Protein recruitment to dynamic DNA-RNA host condensates
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 4, 2024
Abstract
We
describe
the
design
and
characterization
of
artificial
nucleic
acid
condensates
that
are
engineered
to
recruit
locally
concentrate
proteins
interest
in
vitro
.
These
emerge
from
programmed
interactions
nanostructured
motifs
assembling
three
DNA
strands
one
RNA
strand
can
include
an
aptamer
domain
for
recruitment
a
target
protein.
Because
designed
form
regardless
presence
protein,
they
function
as
“host”
compartments.
As
model
protein
we
consider
streptavidin
(SA)
due
its
widespread
use
binding
assays,
thus
host
presented
here
could
find
immediate
physical
separation
variety
biotin-tagged
components.
In
addition
demonstrating
recruitment,
two
approaches
control
onset
condensation
recruitment.
The
first
approach
uses
UV
irradiation,
stimulus
bypasses
need
exchanging
molecular
inputs
is
particularly
convenient
emulsion
droplets.
second
transcription,
ubiquitous
biochemical
reaction
central
development
next
generation
living
materials.
finally
show
combination
transcription
degradation
leads
autonomous
dissipative
system
which
occur
transiently,
condensate
size
well
timescale
transient
be
controlled
by
level
degrading
enzyme.
For
Table
Contents
Only
Язык: Английский