Similarities between decapod and insect neuropeptidomes

PeerJ, Год журнала: 2016, Номер 4, С. e2043 - e2043

Опубликована: Май 26, 2016

Background. Neuropeptides are important regulators of physiological processes and behavior. Although they tend to be generally well conserved, recent results using trancriptome sequencing on decapod crustaceans give the impression significant differences between species, raising question whether such real or artefacts. Methods. The BLAST+ program was used find short reads coding neuropeptides neurohormons in publicly available read archives. Such were then similar same archives, DNA assembly Trinity employed construct contigs encoding neuropeptide precursors as completely possible. Results. seven species analyzed this fashion, crabs Eriocheir sinensis, Carcinus maenas Scylla paramamosain , shrimp Litopenaeus vannamei lobster Homarus americanus fresh water prawn Macrobrachium rosenbergii crayfish Procambarus clarkii had remarkably neuropeptidomes. some could not assembled, many cases individual pertaining missing show unambiguously that these present species. In other cases, tissues express those construction cDNA libraries. One novel identified: elongated PDH (pigment dispersing hormone), a variation has two-amino-acid insertion its core sequence. Hyrg is another peptide ubiquitously decapods likely precursor. Discussion. Many insect have lost one more genes, but apart from hyrg all at least allatotropin only decapods. This strong similarity neuropeptidomes makes it possible predict receptors for been deorphanized insects. includes androgenic insulin-like seems homologous drosophila 8.

Язык: Английский

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Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus

Scientific Reports, Год журнала: 2016, Номер 6(1)

Опубликована: Дек. 7, 2016

Abstract The Australian redclaw crayfish ( Cherax quadricarinatus ) has recently received attention as an emerging candidate for sustainable aquaculture production in Australia and worldwide. More importantly, C. serves a good model organism the commercially important group of decapod crustaceans it is distributed worldwide, easy to maintain laboratory its reproductive cycle been well documented. In order better understand key reproduction development regulating mechanisms crustaceans, molecular toolkit available organisms such must be expanded. However, there no study undertaken establish neuropeptidome. Here we report comprehensive neuropeptide genes expressed eyestalk . We characterised 53 putative neuropeptide-encoding transcripts based on features neuropeptides other species. Of those, 14 implicated regulation were chosen assessment their tissue distribution using RT-PCR. Further insights are discussed relation current knowledge species potential follow up studies. Overall, resulting data lays foundation future gene-based neuroendocrinology studies

Язык: Английский

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A brief and updated introduction to the neuroendocrine system of crustaceans

Molecular and Cellular Endocrinology, Год журнала: 2024, Номер 590, С. 112265 - 112265

Опубликована: Май 1, 2024

Язык: Английский

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Molecular mechanisms of sex determination and differentiation in decapod crustaceans for potential aquaculture applications: An overview

Reviews in Aquaculture, Год журнала: 2024, Номер 16(4), С. 1819 - 1839

Опубликована: Май 23, 2024

Abstract Crustacean aquaculture, especially monosex culture, requires a comprehensive knowledge of the mechanisms regulating sex determination and differentiation. Decapod crustaceans display either female heterogamety (ZW/ZZ) or male (XY/XX) as their mode genotypic determination. However, some decapod species exhibit sexual plasticity via removing androgenic gland, knockdown insulin receptor transcripts, administrating exogenous steroids, altering environmental factors. Although master sex‐determining gene in decapods remains elusive, various genes, hormones, neurotransmitter are emerging important factors This review looks into recent advancements studies related to genetic system that trigger reversal. Moreover, elucidation role differentiation both females males, well underlying interrelated regulatory mechanisms, provides justification for three levels application: induction populations, detection precocious genotype, prevention uncontrolled reproduction. Finally, we proposed future directions bioinformatics analysis genome editing coupled with control increase yield economic decapods. In conclusion, this foundation research on reproductive biology regulation decapods, aim aiding practitioners advancing crustacean aquaculture industry.

Язык: Английский

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Allatostatins C, double C and triple C, the result of a local gene triplication in an ancestral arthropod

General and Comparative Endocrinology, Год журнала: 2016, Номер 230-231, С. 153 - 157

Опубликована: Апрель 21, 2016

Язык: Английский

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Transcriptome Analysis and Differential Gene Expression on the Testis of Orange Mud Crab, Scylla olivacea, during Sexual Maturation

PLoS ONE, Год журнала: 2017, Номер 12(1), С. e0171095 - e0171095

Опубликована: Янв. 30, 2017

Adequate genetic information is essential for sustainable crustacean fisheries and aquaculture management. The commercially important orange mud crab, Scylla olivacea, prevalent in Southeast Asia region highly sought after. Although it a suitable candidate, full domestication of this species hampered by the lack knowledge about sexual maturation process molecular mechanisms behind it, especially males. To date, data on its whole genome yet to be reported S. olivacea. available transcriptome published previously focus primarily females role central nervous system reproductive development. De novo sequencing testes olivacea from immature, maturing mature stages were performed. A total approximately 144 million high-quality reads generated de assembled into 160,569 transcripts with length 142.2 Mb. Approximately 15-23% annotated when compared public protein sequence databases (i.e. UniProt database, Interpro Pfam database Drosophila melanogaster database), GO-categorised GO Ontology terms. 156,181 Single-Nucleotide Polymorphisms (SNPs) mined present study. Transcriptome comparison among different revealed one gene (beta crystallin like gene) most significant differential expression-up-regulated immature stage down-regulated stages. This was further validated qRT-PCR. In conclusion, comprehensive testis crabs obtained. report provides an invaluable resource enhancing our understanding species' structure biology, as expressed controlled their gonads.

Язык: Английский

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Insights Into Sexual Maturation and Reproduction in the Norway Lobster (Nephrops norvegicus) via in silico Prediction and Characterization of Neuropeptides and G Protein-coupled Receptors

Frontiers in Endocrinology, Год журнала: 2018, Номер 9

Опубликована: Июль 27, 2018

Multiple biological processes across development and reproduction are modulated by neuropeptides that predominantly produced secreted from an animal's central nervous system. In the past few years, advancement of next-generation sequencing technologies has enabled large-scale prediction putative neuropeptide genes in multiple non-model species, including commercially important decapod crustaceans. contrast, knowledge G protein-coupled receptors (GPCRs), through which act on target cells, is still very limited. current study, we have used silico transcriptome analysis to elucidate encoding GPCRs Norway lobster (Nephrops norvegicus), one most valuable crustaceans Europe. Fifty-seven precursor-encoding transcripts were detected, phoenixin, a vertebrate neurohormone not been detected any invertebrate species prior this study. Neuropeptide gene expression immature mature female N. norvegicus, revealed some reproduction-related almost exclusively expressed females. addition, total 223 GPCR-encoding identified, 116 encode GPCR-A (Rhodopsin), 44 GPCR-B (Secretin) 63 other GPCRs. Our findings increase molecular toolbox neural signaling components allowing for further advances fisheries/larvae culture species.

Язык: Английский

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Molecular insights into reproduction regulation of female Oriental River prawns Macrobrachium nipponense through comparative transcriptomic analysis

Scientific Reports, Год журнала: 2017, Номер 7(1)

Опубликована: Сен. 18, 2017

Abstract The oriental river prawn, Macrobrachium nipponense , is an important commercial aquaculture resource in China. During breeding season, short ovary maturation cycles of female prawns cause multi-generation reunions ponds and affect the growth females representing individual miniaturization (known as autumn -propagation). These reproductive characteristics pose problems for large - scale farming. To date, molecular mechanisms reproduction regulation M . remain unclear. address this issue, we performed transcriptome sequencing gene expression analyses eyestalk cerebral ganglia M. during non-breeding seasons. Differentially expressed enrichment analysis results revealed several related terms signaling pathways, such “photoreceptor activity”, “structural constituent cuticle” “G-protein coupled receptor activity”. following six key genes from were predicted to mediate environmental factors regulating : neuroparsin, neuropeptide F II, orcokinin crustacean cardioactive peptide, pigment-dispersing hormone 3 tachykinin. will contribute a better understanding prawns. Further detailed functional candidate neuropeptides are needed shed light on crustacean.

Язык: Английский

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Prediction of Scylla olivacea (Crustacea; Brachyura) peptide hormones using publicly accessible transcriptome shotgun assembly (TSA) sequences

General and Comparative Endocrinology, Год журнала: 2016, Номер 230-231, С. 1 - 16

Опубликована: Март 7, 2016

Язык: Английский

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Expansion of the neuropeptidome of the globally invasive marine crab Carcinus maenas

General and Comparative Endocrinology, Год журнала: 2016, Номер 235, С. 150 - 169

Опубликована: Май 11, 2016

Язык: Английский

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