Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Progress in Neurobiology, Год журнала: 2019, Номер 179, С. 101607 - 101607

Опубликована: Март 22, 2019

Язык: Английский

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Hormonal axes in Drosophila: regulation of hormone release and multiplicity of actions

Cell and Tissue Research, Год журнала: 2020, Номер 382(2), С. 233 - 266

Опубликована: Авг. 22, 2020

Hormones regulate development, as well many vital processes in the daily life of an animal. Many these hormones are peptides that act at a higher hierarchical level animal with roles organizers globally orchestrate metabolism, physiology and behavior. Peptide can on multiple peripheral targets simultaneously convey basal states, such metabolic status sleep-awake or arousal across central neuronal circuits. Thereby, they coordinate responses to changing internal external environments. The activity neurosecretory cells is controlled either by (1) cell autonomous sensors, (2) other neurons relay signals from sensors tissues (3) feedback target cells. Thus, hormonal signaling axis commonly comprises several components. In mammals vertebrates, axes known, hypothalamic-pituitary-gonad hypothalamic-pituitary-thyroid reproduction respectively. It has been proposed basic organization evolutionarily old cellular homologs hypothalamic-pituitary system be found for instance insects. To obtain appreciation similarities between insect vertebrate axes, we review systems Drosophila. Our outlines major peptidergic pathways known Drosophila presents set schemes orchestrating systems. detailed larval adult displays only very those arthropods vertebrates.

Язык: Английский

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Signaling Pathways That Regulate the Crustacean Molting Gland

Frontiers in Endocrinology, Год журнала: 2021, Номер 12

Опубликована: Июнь 21, 2021

A pair of Y-organs (YOs) are the molting glands decapod crustaceans. They synthesize and secrete steroid hormones (ecdysteroids) their activity is controlled by external internal signals. The YO transitions through four physiological states over molt cycle, which mediated molt-inhibiting hormone (MIH; basal state), mechanistic Target Rapamycin Complex 1 (mTORC1; activated Transforming Growth Factor-β (TGFβ)/Activin (committed ecdysteroid (repressed state) signaling pathways. MIH, produced in eyestalk X-organ/sinus gland complex, inhibits synthesis ecdysteroids. model for MIH organized into a cAMP/Ca

Язык: Английский

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Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus

Scientific Reports, Год журнала: 2016, Номер 6(1)

Опубликована: Дек. 7, 2016

Abstract The Australian redclaw crayfish ( Cherax quadricarinatus ) has recently received attention as an emerging candidate for sustainable aquaculture production in Australia and worldwide. More importantly, C. serves a good model organism the commercially important group of decapod crustaceans it is distributed worldwide, easy to maintain laboratory its reproductive cycle been well documented. In order better understand key reproduction development regulating mechanisms crustaceans, molecular toolkit available organisms such must be expanded. However, there no study undertaken establish neuropeptidome. Here we report comprehensive neuropeptide genes expressed eyestalk . We characterised 53 putative neuropeptide-encoding transcripts based on features neuropeptides other species. Of those, 14 implicated regulation were chosen assessment their tissue distribution using RT-PCR. Further insights are discussed relation current knowledge species potential follow up studies. Overall, resulting data lays foundation future gene-based neuroendocrinology studies

Язык: Английский

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Transcriptomic analysis of crustacean neuropeptide signaling during the moult cycle in the green shore crab, Carcinus maenas

BMC Genomics, Год журнала: 2018, Номер 19(1)

Опубликована: Сен. 26, 2018

Ecdysis is an innate behaviour programme by which all arthropods moult their exoskeletons. The complex suite of interacting neuropeptides that orchestrate ecdysis well studied in insects, but details the crustacean cassette are fragmented and our understanding this process comparatively crude, preventing a meaningful evolutionary comparison. To begin to address issue we identified transcripts coding for putative receptors central nervous system (CNS) Y-organs (YO) within crab, Carcinus maenas, mapped expression profiles across accurately defined stages cycle using RNA-sequencing. We also gene epidermally-derived YO, only role synthesis ecdysteroid moulting hormones, elucidate peptides G protein-coupled (GPCRs) might have function ecdysis. Transcriptome mining CNS transcriptome yielded neuropeptide representing 47 families 66 GPCRs. Neuropeptide were differentially expressed included carcikinin, hyperglycemic hormone-2, cardioactive peptide, whilst single receptor, proctolin R1, was expressed. Carcikinin mRNA particular exhibited dramatic increases pre-moult, suggesting regulation. Crustacean hormone-2 elevated post- pre-moult regulates insect plays stereotyped motor activity during ecdysis, pre-moult. In several receptor cycle, as neuropeptide, neuroparsin-1. Whilst differential expected, discovery surprising. Analysis GPCR transcript between YO epidermis revealed 11 be upregulated thus now candidates peptide control data presented represent comprehensive survey deduced C. maenas neuropeptidome Importantly, described these staged cycles tissues key programme. This study provides important avenues future exploration functionality receptor-ligand pairs crustaceans.

Язык: Английский

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The biology and evolution of the Dilp8-Lgr3 pathway: A relaxin-like pathway coupling tissue growth and developmental timing control

Mechanisms of Development, Год журнала: 2018, Номер 154, С. 44 - 50

Опубликована: Апрель 30, 2018

Язык: Английский

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Coleoptera genome and transcriptome sequences reveal numerous differences in neuropeptide signaling between species

PeerJ, Год журнала: 2019, Номер 7, С. e7144 - e7144

Опубликована: Июнь 17, 2019

Insect neuropeptides are interesting for the potential their receptors hold as plausible targets a novel generation of pesticides. Neuropeptide genes have been identified in number different species belonging to variety insects. Results suggest significant neuropeptide variation between orders, but much less is known neuropeptidome variability within an insect order. I therefore compared neuropeptidomes Coleoptera.Publicly available genome sequences, transcriptomes and original sequence data form short read archives were analyzed presence or absence coding well some seventeen beetle species.Significant differences exist Coleoptera here, while many that previously characterized from Tribolium castaneum appear very similar all species, not others lacking one more species. On other hand, leucokinin, which was presumed be universally absent Coleoptera, still present non-Polyphaga beetles.The composition same order may large exists orders.

Язык: Английский

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Arthropod IGF, relaxin and gonadulin, putative orthologs of Drosophila insulin-like peptides 6, 7 and 8, likely originated from an ancient gene triplication

PeerJ, Год журнала: 2020, Номер 8, С. e9534 - e9534

Опубликована: Июль 10, 2020

Background Insects have several genes coding for insulin-like peptides and they been particularly well studied in Drosophila . Some of these hormones function as growth are produced by the fat body brain. These act through a typical insulin receptor tyrosine kinase. Two other either known or suspected to G-protein coupled receptor. Although insulin-related from insect species, peptide 8, one that uses receptor, has so far only identified flies. However, its is widespread within arthropods hence it should orthologs. Such putative orthologs were recently decapods called gonadulins. Methodology In an effort identify gonadulins public genome assemblies short-read archives insects explored presence transcripts their receptors. Results Gonadulins detected number arthropods. those species which transcriptome data gonads available gonadulin expressed ovaries at least some also testes. differences expression ovary between actively reproducing non-reproducing females differs more than 100-fold. Putative ilp 6 identified. non-Dipteran C-terminally extensions alternatively spliced. The predicted arthropod factors. cockroaches, termites stick factors, relaxin, third peptide, encoded next another suggesting result local gene triplication. close chromosomal association was found factor spiders. Phylogenetic tree analysis kinases insects, chelicerates shows signaling pathway evolved differently three groups. receptors related 8 similarly show significant Conclusion A triplication early ancestor likely yielded gonadulin, relaxin. Orthologs now commonly present almost certainly include 6, 7 8.

Язык: Английский

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A brief and updated introduction to the neuroendocrine system of crustaceans

Molecular and Cellular Endocrinology, Год журнала: 2024, Номер 590, С. 112265 - 112265

Опубликована: Май 1, 2024

Язык: Английский

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The moulting arthropod: a complete genetic toolkit review

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Год журнала: 2024, Номер 99(6), С. 2338 - 2375

Опубликована: Июль 22, 2024

ABSTRACT Exoskeletons are a defining character of all arthropods that provide physical support for their segmented bodies and appendages as well protection from the environment predation. This ubiquitous yet evolutionarily variable feature has been instrumental in facilitating adoption variety lifestyles exploitation ecological niches across environments. Throughout radiation produced more than one million described modern species, adaptability afforded by segmentation exoskeletons led to diversity is unrivalled amongst animals. However, because limited extensibility exoskeleton chitin cuticle components, they must be periodically shed replaced with new larger ones, notably accommodate growing individuals encased within. Therefore, grow discontinuously undergoing periodic moulting events, which follow series steps preparatory pre‐moult phase ecdysis itself post‐moult maturation exoskeletons. Each event represents particularly vulnerable period an arthropod's life cycle, so processes tightly regulated meticulously executed ensure successful transitions normal growth development. Decades research representative foundation understanding mechanisms involved. Building on this, studies continue develop test hypotheses presence function molecular including neuropeptides, hormones, receptors, so‐called early, late, fate genes, arthropod diversity. Here, we review literature comprehensive overview status accumulated knowledge genetic toolkit governing moulting. From biosynthesis regulation ecdysteroid sesquiterpenoid factors involved hormonal stimulation responses remodelling, identify commonalities differences, highlighting major gaps, groups. We examine available evidence supporting current models how components operate together prepare for, execute, recover ecdysis, comparing reports Chelicerata, Myriapoda, Crustacea, Hexapoda. Evidence generally highly taxonomically imbalanced, most based insect study systems. Biases also evident different phases processes, early triggers late effectors being least explored. Our synthesis contrasts reported observations reasonably plausible assumptions given taxonomic sampling, exposes weak or gaps need addressing. Encouragingly, advances genomics driving diversification tractable systems cataloguing putative toolkits previously under‐explored taxa. Analysis genome transcriptome data supported experimental investigations have validated “ultra‐conserved” core genes processes. The machinery likely evolved elaborations this conserved pathway backbone, but exploration needed characterise lineage‐specific changes novelties. Furthermore, linking these transformative innovations Arthropoda remains hampered untested assumptions. Promisingly however, emerging framework highlights avenues underlying genetics dynamic biology through complex physiology

Язык: Английский

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