Spectral tracing of deuterium for imaging glucose metabolism

Nature Biomedical Engineering, Год журнала: 2019, Номер 3(5), С. 402 - 413

Опубликована: Апрель 29, 2019

Язык: Английский

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Bond-selective imaging by optically sensing the mid-infrared photothermal effect

Science Advances, Год журнала: 2021, Номер 7(20)

Опубликована: Май 14, 2021

Mid-infrared photothermal microscopy enables vibrational spectroscopic imaging at submicrometer spatial resolution.

Язык: Английский

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The Emerging Role of Raman Spectroscopy as an Omics Approach for Metabolic Profiling and Biomarker Detection toward Precision Medicine

Chemical Reviews, Год журнала: 2023, Номер 123(13), С. 8297 - 8346

Опубликована: Июнь 15, 2023

Omics technologies have rapidly evolved with the unprecedented potential to shape precision medicine. Novel omics approaches are imperative toallow rapid and accurate data collection integration clinical information enable a new era of healthcare. In this comprehensive review, we highlight utility Raman spectroscopy (RS) as an emerging technology for clinically relevant applications using significant samples models. We discuss use RS both label-free approach probing intrinsic metabolites biological materials, labeled where signal from reporters conjugated nanoparticles (NPs) serve indirect measure tracking protein biomarkers

Язык: Английский

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Volumetric chemical imaging by clearing-enhanced stimulated Raman scattering microscopy

Proceedings of the National Academy of Sciences, Год журнала: 2019, Номер 116(14), С. 6608 - 6617

Опубликована: Март 14, 2019

Significance Cells form structures and perform functions through intricate 3D tissue organizations. However, due to scattering, coherent Raman microscopy—a powerful method complementary fluorescence imaging—suffers from limited imaging depth in tissues. Here, we develop a volumetric chemical with greater than 10-fold increase. We formulate Raman-tailored tissue-clearing recipe combine it advanced microscopies. Equipped the toolbox of analyses, elucidate complex structures, compositions, metabolic dynamics diverse tissues including lipid synthesis throughout tumor spheroids, networks axons, vasculatures, cell bodies brain regions, as well heterogeneous metabolism.

Язык: Английский

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Raman Imaging of Small Biomolecules

Annual Review of Biophysics, Год журнала: 2019, Номер 48(1), С. 347 - 369

Опубликована: Март 20, 2019

Imaging techniques greatly facilitate the comprehensive knowledge of biological systems. Although imaging methodology for biomacromolecules such as protein and nucleic acids has been long established, microscopic contrast mechanisms are relatively limited small biomolecules, which equally important participants in processes. Recent developments Raman imaging, including both microscopy tailored vibrational tags, have created exciting opportunities noninvasive biomolecules living cells, tissues, organisms. Here, we summarize principle workflow small-biomolecule by microscopy. Then, review recent efforts example, lipids, metabolites, drugs. The unique advantage manifested a variety applications that provided novel insights.

Язык: Английский

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Electronic Preresonance Stimulated Raman Scattering Microscopy

The Journal of Physical Chemistry Letters, Год журнала: 2018, Номер 9(15), С. 4294 - 4301

Опубликована: Июль 12, 2018

Optical microscopy has generated great impact for modern research. While fluorescence provides the ultimate sensitivity, it generally lacks chemical information. Complementarily, vibrational imaging methods provide rich chemical-bond-specific contrasts. Nonetheless, they usually suffer from unsatisfying sensitivity or compromised biocompatibility. Recently, electronic preresonance stimulated Raman scattering (EPR-SRS) was reported, achieving simultaneous high detection and superb specificity of chromophores. With newly synthesized Raman-active dyes, this method readily breaks optical color barrier is well-suited supermultiplex in biological samples. In Perspective, we first review previous utilizations resonance various spectroscopy microscopy. We then discuss physical origin uniqueness region, followed by quantitative analysis enhancement factors involved EPR-SRS On basis, an outlook future development as well broad applications biophotonics.

Язык: Английский

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Multiplex Stimulated Raman Scattering Imaging Cytometry Reveals Lipid-Rich Protrusions in Cancer Cells under Stress Condition

iScience, Год журнала: 2020, Номер 23(3), С. 100953 - 100953

Опубликована: Фев. 29, 2020

In situ measurement of cellular metabolites is still a challenge in biology. Conventional methods, such as mass spectrometry or fluorescence microscopy, would either destroy the sample introduce strong perturbations to target molecules. Here, we present multiplex stimulated Raman scattering (SRS) imaging cytometry label-free single-cell analysis platform with chemical specificity and high-throughput capabilities. Using SRS cytometry, studied metabolic responses human pancreatic cancer cells under stress by starvation chemotherapeutic drug treatments. We unveiled protrusions containing lipid droplets marker for stress-resistant cells. Furthermore, spectroscopic mapping, that triglyceride are used local energy production through lipolysis, autophagy, β-oxidation. Our findings demonstrate potential targeting metabolism selective treatment cancers. Collectively, these results highlight powerful tool biological discoveries high-throughput, high-content capacity.

Язык: Английский

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Multiplexed live-cell profiling with Raman probes

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Июнь 7, 2021

Abstract Single-cell multiparameter measurement has been increasingly recognized as a key technology toward systematic understandings of complex molecular and cellular functions in biological systems. Despite extensive efforts analytical techniques, it is still generally challenging for existing methods to decipher large number phenotypes single living cell. Herein we devise multiplexed Raman probe panel with sharp mutually resolvable peaks simultaneously quantify cell surface proteins, endocytosis activities, metabolic dynamics an individual live When coupling whole-cell spontaneous micro-spectroscopy, demonstrate the utility this technique 14-plexed live-cell profiling phenotyping under various drug perturbations. In particular, single-cell enables powerful clustering, correlation, network analysis insights. This platform compatible cytometry, low instrument complexity capable highly robust straightforward manner, thereby contributing valuable tool both basic biology translation applications such high-content sorting discovery.

Язык: Английский

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Optoacoustic imaging in endocrinology and metabolism

Nature Reviews Endocrinology, Год журнала: 2021, Номер 17(6), С. 323 - 335

Опубликована: Апрель 19, 2021

Язык: Английский

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Surface-enhanced Raman scattering nanotags for bioimaging

Journal of Applied Physics, Год журнала: 2021, Номер 129(19)

Опубликована: Май 17, 2021

Surface-enhanced Raman scattering (SERS) technique has shown extraordinary features for biomedical applications. The implementation of SERS nanotags opened a new era bioimaging and detections. As powerful tool, provide favorable properties such as fingerprint spectrum, narrow peak linewidth, good photostability, high spatial resolution accompanied by various rational designs nanoparticles. They have proven useful imaging agents in vivo, ex vitro detection cancerous cells tissues. This tutorial provides the basic principles nanotags, including recent progress SERS-based applications, well outlooks into future developments toward practical clinical SERS.

Язык: Английский

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