Fluorescence imaging of pathophysiological microenvironments

Chemical Society Reviews, Год журнала: 2021, Номер 50(16), С. 8887 - 8902

Опубликована: Янв. 1, 2021

Abnormal microenvironments (viscosity, polarity, pH, etc. ) have been verified to be closely associated with numerous pathophysiological processes such as inflammation, neurodegenerative diseases, and cancer.

Язык: Английский

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Fluorescent small organic probes for biosensing

Chemical Science, Год журнала: 2021, Номер 12(10), С. 3406 - 3426

Опубликована: Янв. 1, 2021

Small-molecule based fluorescent probes are increasingly important for the detection and imaging of biological signaling molecules due to their simplicity, high selectivity sensitivity, whilst being non-invasive, suitable real-time analysis living systems. With this perspective we highlight sensing mechanisms including Förster resonance energy transfer (FRET), intramolecular charge (ICT), photoinduced electron (PeT), excited state proton (ESIPT), aggregation induced emission (AIE) multiple modality fluorescence approaches dual/triple (DSM or TSM). Throughout remaining challenges suggest potential directions development towards improved small-molecule biosensing.

Язык: Английский

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Activity-based NIR fluorescent probes based on the versatile hemicyanine scaffold: design strategy, biomedical applications, and outlook

Chemical Society Reviews, Год журнала: 2022, Номер 51(5), С. 1795 - 1835

Опубликована: Янв. 1, 2022

The discovery of a near-infrared (NIR, 650-900 nm) fluorescent chromophore hemicyanine dye with high structural tailorability is great significance in the field detection, bioimaging, and medical therapeutic applications. It exhibits many outstanding advantages including absorption emission NIR region, tunable spectral properties, photostability as well large Stokes shift. These properties are superior to those conventional fluorogens, such coumarin, fluorescein, naphthalimides, rhodamine, cyanine. Researchers have made remarkable progress developing activity-based multifunctional probes based on skeletons for monitoring vital biomolecules living systems through output fluorescence/photoacoustic signals, integration diagnosis treatment diseases using chemotherapy or photothermal/photodynamic therapy combination therapy. achievements prompted researchers develop more smart fluorogen template. In this review, we begin by describing brief history dyes, synthetic approaches, design strategies functional probes. Then, selected hemicyanine-based that can detect ions, small biomolecules, overexpressed enzymes diagnostic reagents systematically highlighted. Finally, potential drawbacks outlook future investigation clinical medicine transformation activatable also discussed.

Язык: Английский

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BODIPY‐Based Fluorescent Probes for Biothiols

Chemistry - A European Journal, Год журнала: 2019, Номер 26(19), С. 4172 - 4192

Опубликована: Ноя. 26, 2019

Abstract Fluorescent probes for biothiols have aroused increasing interest owing to their potential enable better understanding of the diverse physiological and pathological processes related biothiol species. BODIPY fluorophores exhibit excellent optical properties, which can be readily tailored by introducing functional units at various positions core. In present review, development fluorescent based on BODIPYs detection are systematically summarized, with emphasis preferable individual biothiols, as well simultaneous discrimination among cysteine (Cys), homocysteine (Hcy), reduced glutathione (GSH). addition, organelle‐targeting also highlighted. The general design principles, recognition mechanisms, biological applications elaboratively discussed, could provide a useful reference researchers worldwide interested in this area.

Язык: Английский

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Recent progress in fluorescent probes for detection of carbonyl species: Formaldehyde, carbon monoxide and phosgene

Coordination Chemistry Reviews, Год журнала: 2019, Номер 404, С. 213109 - 213109

Опубликована: Ноя. 11, 2019

Язык: Английский

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Recent progress in the development of fluorescent probes for detection of biothiols

Dyes and Pigments, Год журнала: 2020, Номер 177, С. 108321 - 108321

Опубликована: Март 1, 2020

Язык: Английский

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Direct Quantification and Visualization of Homocysteine, Cysteine, and Glutathione in Alzheimer’s and Parkinson’s Disease Model Tissues

Analytical Chemistry, Год журнала: 2021, Номер 93(28), С. 9878 - 9886

Опубликована: Июль 7, 2021

Alzheimer's disease (AD) and Parkinson's (PD) are chronic neurodegenerative diseases with high morbidity mortality. Homocysteine (Hcy), cysteine (Cys), glutathione (GSH) closely related to AD PD. However, the dynamics of Hcy, Cys, GSH in brain tissues potential pathogenesis between Cys/Hcy/GSH PD remain unclear. Herein, a novel fluorescent probe 1 multiple binding sites was rationally designed exploited for direct quantification serum total Hcy Cys along superior optical properties. Importantly, differentiation simultaneity fluorescence imaging were achieved living cells, tissues, mouse models this probe, providing evidences relationship Hcy/Cys/GSH AD/PD first time. In addition, studies demonstrated that elevated levels imbalanced redox homeostasis, increased amyloid aggregates, nerve cell cytotoxicity. These findings will greatly promote understanding functions diseases, demonstrating clinical promise early diagnosis prevention

Язык: Английский

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Glutathione-mediated nanomedicines for cancer diagnosis and therapy

Chemical Engineering Journal, Год журнала: 2021, Номер 426, С. 128880 - 128880

Опубликована: Фев. 11, 2021

Язык: Английский

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Quasi-LD-Targeted and ONOO^–-Responsive Fluorescent Probe for Investigating the Interaction of Nonalcoholic Fatty Liver with Drug-Induced Liver Injury

Analytical Chemistry, Год журнала: 2023, Номер 95(14), С. 5967 - 5975

Опубликована: Март 29, 2023

Hepatic lipid droplets (LDs) and peroxynitrite (ONOO-) levels are closely related to nonalcoholic fatty liver disease (NAFLD). Additionally, some drug-induced injury (DILI) is often associated with ONOO-. Here, we constructed screened the quasi-LDs-targeted ONOO--responsive fluorescent probe MBDP-Py+ investigate interaction of NAFLD DILI. By monitoring upregulation ONOO- accumulation LDs, was more sensitive efficient than tissue staining serum markers detection in evaluating early toxicity diagnosing anticancer-DILI. More importantly, enhancement fluorescence signals demonstrated that different stages NAFLD, dominant element combined DILI mice models. As degree deepens, synergistic effect two will lead serious damage.

Язык: Английский

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Recent advances in small-molecule fluorescent probes for studying ferroptosis

Chemical Society Reviews, Год журнала: 2022, Номер 51(18), С. 7752 - 7778

Опубликована: Янв. 1, 2022

Ferroptosis is an iron-dependent, non-apoptotic form of programmed cell death driven by excessive lipid peroxidation (LPO). Mounting evidence suggests that the unique modality involved in development and progression several diseases including cancer, cardiovascular (CVDs), neurodegenerative disorders, etc. However, pathogenesis signalling pathways ferroptosis are not fully understood, possibly due to lack robust tools for highly selective sensitive imaging analytes complex living systems. Up now, various small-molecule fluorescent probes have been applied as promising chemosensors studying through tracking biomolecules or microenvironment-related parameters vitro vivo. In this review, we comprehensively reviewed recent ferroptosis, with a focus on analytes, design strategies bioimaging applications. We also provided new insights overcome major challenges emerging field.

Язык: Английский

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