Chemical Science,
Год журнала:
2020,
Номер
12(10), С. 3379 - 3392
Опубликована: Июнь 18, 2020
Near-infrared
(NIR)
fluorescence
imaging
has
improved
depth
relative
to
conventional
in
the
visible
region,
demonstrating
great
potential
both
fundamental
biomedical
research
and
clinical
practice.
To
improve
detection
specificity,
NIR
probes
have
been
under
extensive
development.
This
review
summarizes
particular
application
of
optical
with
NIR-I
window
(700-900
nm)
or
NIR-II
(1000-1700
emission
for
diagnosis
nephron-urological
diseases.
These
molecular
enabled
contrast-enhanced
anatomical
structures
physiological
function
as
well
early
acute
kidney
injury,
iatrogenic
ureteral
injury
bladder
cancer.
The
design
strategies
are
specifically
elaborated
along
representative
applications.
challenges
perspectives
this
field
also
discussed.
Chemical Society Reviews,
Год журнала:
2021,
Номер
50(21), С. 12098 - 12150
Опубликована: Янв. 1, 2021
The
microenvironment
(local
environment),
including
viscosity,
temperature,
polarity,
hypoxia,
and
acidic-basic
status
(pH),
plays
indispensable
roles
in
cellular
processes.
Significantly,
organelles
require
an
appropriate
to
perform
their
specific
physiological
functions,
disruption
of
the
microenvironmental
homeostasis
could
lead
malfunctions
organelles,
resulting
disorder
disease
development.
Consequently,
monitoring
within
is
vital
understand
organelle-related
physiopathology.
Over
past
few
years,
many
fluorescent
probes
have
been
developed
help
reveal
variations
regions.
Given
that
a
comprehensive
understanding
particular
region
great
significance
for
further
exploration
life
events,
thorough
summary
this
topic
urgently
required.
However,
there
has
not
critical
review
published
recently
on
small-molecule
chemosensors
microenvironment.
With
review,
we
summarize
recent
progress
since
2015
towards
based
imaging
regions,
mitochondria,
lysosomes,
lipid
drops,
endoplasmic
reticulum,
golgi,
nucleus,
cytoplasmic
matrix
cell
membrane.
Further
classifications
at
suborganelle
level,
according
detection
factors
by
probes,
pH
are
presented.
Notably,
each
category,
design
principles,
chemical
synthesis,
recognition
mechanism,
signals,
bio-imaging
applications
summarized
compared.
In
addition,
limitations
current
microenvironment-sensitive
analyzed
prospects
future
developments
outlined.
nutshell,
comprehensively
summarizes
highlights
small
molecule
sensing
regions
2015.
We
anticipate
will
facilitate
deeper
encourage
research
directed
development
microenvironments.
ACS Nano,
Год журнала:
2020,
Номер
14(9), С. 11017 - 11028
Опубликована: Авг. 13, 2020
Although
ferroptosis
therapy
has
been
proven
to
be
a
promising
strategy
for
cancer
treatment,
its
efficacy
still
might
limited
by
insufficient
H2O2
supply
in
tumor
tissue.
Herein,
we
designed
cell
membrane-cloaked
cascade
nanoreactor
based
on
ferric
metal–organic
frameworks
(MOF)
and
glucose
oxidase
(GOx)
decoration
synergistic
ferroptosis–starvation
anticancer
therapy.
The
GOx
can
catalyze
generate
sufficient
therapy,
the
consumption
caused
utilized
as
another
attractive
treatment
called
starvation
When
reached
sites,
high
concentration
of
GSH
reduced
Fe3+
trigger
structure
collapse
MOF
release
Fe2+
catalyzed
oxidation
H2O2.
Then
Fenton
reaction
happened
between
produce
hydroxyl
radicals
(•OH)
promoted
With
these
reactions,
was
realized.
Furthermore,
membrane
endows
homologous
targeting
immune
escaping
ability,
which
facilitated
accumulate
into
site
with
efficiency.
exhibits
efficiency
suppression
situ
consumed
produced
compounds,
promote
development
precise
cooperative
spatiotemporal
controllability.
Journal of the American Chemical Society,
Год журнала:
2021,
Номер
143(2), С. 912 - 924
Опубликована: Янв. 8, 2021
Biomembranes
constitute
a
basis
for
all
compartments
of
live
cells,
and
therefore,
the
monitoring
their
lipid
organization
is
essential
understanding
cell
status
activity.
However,
sensing
imaging
specifically
in
different
organelles
cells
remain
challenging.
Here,
we
designed
an
array
solvatochromic
probes
based
on
Nile
Red
bearing
ligands
specific
targeting
endoplasmic
reticulum,
mitochondria,
lysosomes,
Golgi
apparatus,
plasma
membranes,
droplets.
These
polarity-sensitive
detected
variations
order
by
changing
emission
maximum,
as
evidenced
fluorescence
spectroscopy
model
membranes.
In
colocalization
microscopy
experiments
with
reference
organelle
markers,
they
exhibited
good
selectivity.
Using
two-color
microscopy,
new
enabled
local
polarity
cells.
To
exclude
biased
effect
probe
design
sensitivity
to
membrane
properties,
calibrated
membranes
under
microscope,
which
first
quantitative
description
each
interest.
Cholesterol
extraction/enrichment
confirmed
capacity
sense
order,
revealing
that
poor
cholesterol
are
particularly
affected
its
enrichment.
The
also
revealed
oxidative
mechanical
stresses
produced
changes
were
characteristic
organelle,
mitochondria
lysosomes
being
stress
sensitive.
powerful
toolbox
response
physical
chemical
stimuli
at
level
individual
organelles,
remains
underexplored
direction
cellular
research.
Chemistry - A European Journal,
Год журнала:
2019,
Номер
26(19), С. 4172 - 4192
Опубликована: Ноя. 26, 2019
Abstract
Fluorescent
probes
for
biothiols
have
aroused
increasing
interest
owing
to
their
potential
enable
better
understanding
of
the
diverse
physiological
and
pathological
processes
related
biothiol
species.
BODIPY
fluorophores
exhibit
excellent
optical
properties,
which
can
be
readily
tailored
by
introducing
functional
units
at
various
positions
core.
In
present
review,
development
fluorescent
based
on
BODIPYs
detection
are
systematically
summarized,
with
emphasis
preferable
individual
biothiols,
as
well
simultaneous
discrimination
among
cysteine
(Cys),
homocysteine
(Hcy),
reduced
glutathione
(GSH).
addition,
organelle‐targeting
also
highlighted.
The
general
design
principles,
recognition
mechanisms,
biological
applications
elaboratively
discussed,
could
provide
a
useful
reference
researchers
worldwide
interested
in
this
area.
Angewandte Chemie International Edition,
Год журнала:
2021,
Номер
60(31), С. 16763 - 16776
Опубликована: Март 9, 2021
Abstract
Metal–organic
frameworks
(MOFs)
hold
great
promise
for
biomedical
applications
owing
to
their
unique
properties.
The
porous
structures
make
MOFs
excellent
candidates
the
delivery
of
different
drugs;
flexibility
in
choosing
metal
ions
and
organic
ligands
makes
it
feasible
prepare
with
intrinsic
antitumor
activities
further
devise
MOF–drug
synergistic
systems;
many
other
types
agents
could
also
be
developed
using
as
precursors/templates.
Thus,
past
two
decades
have
witnessed
development
MOF‐based
drugs,
especially
field.
This
Minireview
mainly
focuses
on
design
agents.
Four
aspects
covering
whole
field
are
introduced:
carriers,
agents,
systems,
MOF‐derived
challenges
opportunities
clinical
discussed.
Chemical Society Reviews,
Год журнала:
2023,
Номер
52(7), С. 2322 - 2357
Опубликована: Янв. 1, 2023
Typical
PeT-based
fluorescent
probes
are
multi-component
systems
where
a
fluorophore
is
connected
to
recognition/activating
group
by
an
unconjugated
linker.
powerful
tools
for
cell
imaging
and
disease
diagnosis
due
their
low
fluorescence
background
significant
enhancement
towards
the
target.
This
review
provides
research
progress
that
target
polarity,
pH
biological
species
(reactive
oxygen
species,
biothiols,
biomacromolecules,
etc.)
over
last
five
years.
In
particular,
we
emphasise
molecular
design
strategies,
mechanisms,
application
of
these
probes.
As
such,
this
aims
provide
guidance
enable
researchers
develop
new
improved
probes,
as
well
promoting
use
sensing,
imaging,
therapy.
Nature Communications,
Год журнала:
2020,
Номер
11(1)
Опубликована: Дек. 8, 2020
Abstract
Mitochondria–lysosome
interactions
are
essential
for
maintaining
intracellular
homeostasis.
Although
various
fluorescent
probes
have
been
developed
to
visualize
such
interactions,
they
remain
unable
label
mitochondria
and
lysosomes
simultaneously
dynamically
track
their
interaction.
Here,
we
introduce
a
cell-permeable,
biocompatible,
viscosity-responsive,
small
organic
molecular
probe,
Coupa,
monitor
the
interaction
of
in
living
cells.
Through
functional
fluorescence
conversion,
Coupa
can
with
blue
red
fluorescence,
correlation
between
red–blue
intensity
indicates
progress
mitochondria–lysosome
interplay
during
mitophagy.
Moreover,
because
its
is
sensitive
viscosity,
allowed
us
precisely
localize
sites
contact
reveal
increases
local
viscosity
on
associated
contact.
Thus,
our
probe
represents
an
attractive
tool
localization
dynamic
tracking
Chemical Communications,
Год журнала:
2021,
Номер
58(10), С. 1495 - 1509
Опубликована: Дек. 22, 2021
This
feature
article
discusses
the
recent
advances
in
design
strategies,
structural
features
and
applications
of
fluorescent
probes
for
lipid
droplets.
