Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(26)
Опубликована: Апрель 19, 2024
Fluorescence
sensing
is
crucial
to
studying
biological
processes
and
diagnosing
diseases,
especially
in
the
second
near-infrared
(NIR-II)
window
with
reduced
background
signals.
However,
it's
still
a
great
challenge
construct
"off-on"
sensors
when
wavelength
extends
into
NIR-II
region
obtain
higher
imaging
contrast,
mainly
due
difficult
synthesis
of
spectral
overlapped
quencher.
Here,
we
present
new
fluorescence
quenching
strategy,
which
utilizes
steric
hindrance
quencher
(SHQ)
tune
molecular
packing
state
fluorophores
suppress
emission
signal.
Density
functional
theory
(DFT)
calculations
further
reveal
that
large
SHQs
can
competitively
pack
prevent
their
self-aggregation.
Based
on
this
mechanism,
novel
activatable
method
achieved
via
bio-analyte
responsive
invalidation
SHQ,
namely
Steric
Hindrance
Invalidation
geNerated
Emission
(SHINE)
strategy.
As
proof
concept,
ClO
Chemical Science,
Год журнала:
2023,
Номер
14(22), С. 5842 - 5871
Опубликована: Янв. 1, 2023
Biological
species,
including
reactive
oxygen
species
(ROS),
sulfur
(RSS),
nitrogen
(RNS),
F
−
,
Pd
2+
Cu
Hg
and
others,
are
crucial
for
the
healthy
functioning
of
cells
in
living
organisms.
ACS Chemical Biology,
Год журнала:
2021,
Номер
16(8), С. 1304 - 1317
Опубликована: Июль 27, 2021
Proteases
are
enzymes
capable
of
catalyzing
protein
breakdown,
which
is
critical
across
many
biological
processes.
There
several
families
proteases,
each
perform
key
functions
through
the
degradation
specific
proteins.
As
our
understanding
cancer
improves,
it
has
been
demonstrated
that
proteases
can
be
overactivated
during
progression
and
contribute
to
malignancy.
Optical
imaging
systems
employ
near-infrared
(NIR)
fluorescent
probes
detect
protease
activity
offer
clinical
promise,
both
for
early
detection
as
well
assessment
personalized
therapy.
In
this
Review,
we
review
design
NIR
their
successful
application
different
cancer-associated
proteases.
Angewandte Chemie International Edition,
Год журнала:
2022,
Номер
62(1)
Опубликована: Сен. 30, 2022
Decoding
cellular
processes
requires
visualization
of
the
spatial
distribution
and
dynamic
interactions
biomolecules.
It
is
therefore
not
surprising
that
innovations
in
imaging
technologies
have
facilitated
advances
biomedical
research.
The
advent
super-resolution
has
empowered
researchers
with
ability
to
answer
long-standing
questions
about
at
an
entirely
new
level.
Fluorescent
probes
greatly
enhance
specificity
resolution
experiments.
Here,
we
introduce
key
technologies,
a
brief
discussion
on
single-molecule
localization
microscopy
(SMLM).
We
evaluate
chemistry
photochemical
mechanisms
fluorescent
employed
SMLM.
This
Review
provides
guidance
identification
adoption
single
molecule
inspire
design
next-generation
amenable
imaging.
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(27), С. 14647 - 14659
Опубликована: Июнь 27, 2023
Heptamethine
indocyanines
are
invaluable
probes
for
near-infrared
(NIR)
imaging.
Despite
broad
use,
there
only
a
few
synthetic
methods
to
assemble
these
molecules,
and
each
has
significant
limitations.
Here,
we
report
the
use
of
pyridinium
benzoxazole
(PyBox)
salts
as
heptamethine
indocyanine
precursors.
This
method
is
high
yielding,
simple
implement,
provides
access
previously
unknown
chromophore
functionality.
We
applied
this
create
molecules
address
two
outstanding
objectives
in
NIR
fluorescence
First,
used
an
iterative
approach
develop
protein-targeted
tumor
When
compared
common
fluorophores,
optimized
probe
increases
specificity
monoclonal
antibody
(mAb)
nanobody
conjugates.
Second,
developed
cyclizing
with
goal
improving
cellular
uptake
fluorogenic
properties.
By
modifying
both
electrophilic
nucleophilic
components,
demonstrate
that
solvent
sensitivity
ring-open/ring-closed
equilibrium
can
be
modified
over
wide
range.
then
show
chloroalkane
derivative
compound
tuned
cyclization
properties
undergoes
particularly
efficient
no-wash
live
cell
imaging
using
organelle-targeted
HaloTag
self-labeling
proteins.
Overall,
chemistry
reported
here
broadens
scope
accessible
functionality,
and,
turn,
enables
discovery
promising
advanced
applications.
Chemical Science,
Год журнала:
2023,
Номер
14(8), С. 2139 - 2148
Опубликована: Янв. 1, 2023
An
AIE-active
probe
with
NIR
emission
was
designed
to
achieve
ultralong-term
spatiotemporal
imaging
of
the
plasma
membranes
plant
cells,
and
its
long-term
monitoring
ability
wide
applicability
diverse
cells
were
demonstrated.
