Click Conjugation of Zwitterionic Peptide with DNA Strand: An Efficient Antifouling Strategy for Versatile Photoelectrochemical Aptasensor

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Фев. 11, 2024

Advanced antifouling biosensors have garnered considerable attention for their potential precise and sensitive analysis in complex human bodily fluids. Herein, a pioneering approach was utilized to establish robust versatile photoelectrochemical aptasensor by conjugating zwitterionic peptide with DNA strand. Specifically, the branched (BZP) efficiently linked complementary (cDNA) through click reaction, forming BZP–cDNA conjugate. This intriguing conjugate exploited BZP domain create an biointerface, while cDNA component facilitated subsequent hybridization probe (pDNA). To advance development of aptasensor, upgraded PDA/HOF-101/ZnO ternary photoelectrode designed as signal converter modification conjugate, bipyridinium (MCEPy) molecule strong electron-withdrawing properties labeled at front end pDNA form pDNA–MCEPy probe. Targeting model mucin-1, remarkable enhancement photocurrent achieved exonuclease-I-aided target recycling. Such engineered peptide–DNA surpasses limitations imposed conventional peptide-based sensing modes, exhibiting unique advantages such versatility design capability amplification.

Язык: Английский

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CRISPR/Cas detection with nanodevices: moving deeper into liquid biopsy

Chemical Communications, Год журнала: 2024, Номер 60(17), С. 2301 - 2319

Опубликована: Янв. 1, 2024

This feature article summarises the latest advances, potential challenges, and future prospects of CRISPR/Cas gene-editing technologies in conjunction with emerging nanodevices for refinement precision liquid biopsy techniques.

Язык: Английский

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Detection of biological loads in sewage using the automated robot‐driven photoelectrochemical biosensing platform

Exploration, Год журнала: 2024, Номер 4(5)

Опубликована: Март 14, 2024

Abstract Real‐time polymerase chain reaction (RT‐PCR) remains the most prevalent molecular detection technology for sewage analysis but is plagued with numerous disadvantages, such as time consumption, high manpower requirements, and susceptibility to false negatives. In this study, an automated robot‐driven photoelectrochemical (PEC) biosensing platform constructed, that utilizes CRISPR/Cas12a system achieve fast, ultrasensitive, specificity of biological loads in sewage. The Shennong‐1 robot integrates several functional modules, involving sampling pretreatment streamline monitoring. A screen‐printed electrode employed a vertical graphene‐based working enhanced surface‐deposited Au nanoparticles (NPs). CdTe/ZnS quantum dots (QDs) are further fabricated through double‐stranded DNA (dsDNA) anchored on NPs. Using cDNA template Omicron BA.5 spike gene model, PEC biosensor demonstrates excellent analytical performance, lower limit 2.93 × 10 2 z m outstanding selectivity at level single‐base mutation recognition. Furthermore, rapid, accurate feasibility conclusion, allows early tracking infectious disease outbreaks, providing timely data support public health institutions take appropriate prevention control measures.

Язык: Английский

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A triple-amplified electrochemical-photoelectrochemical dual-mode biosensing platform for viral gene assay based on catalytic hairpin assembly, CRISPR-Cas12a and liposome-entrapped bifunctional methylene blue

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 411, С. 135744 - 135744

Опубликована: Апрель 3, 2024

Язык: Английский

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Cell-free systems: A synthetic biology tool for rapid prototyping in metabolic engineering

Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108522 - 108522

Опубликована: Янв. 1, 2025

Язык: Английский

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