Comprehensive insights into pathogenicity and emerging CRISPR-integrated biosensors for the early detection of breast cancer biomarkers

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 112908 - 112908

Опубликована: Янв. 1, 2025

Язык: Английский

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Dual-Accelerated Signal Amplification in Biosensing via Spatial Confining Catalytic Hairpin Assembly-Activated Spherical CRISPR/Cas12a System for Trans-Cleavage of Hairpin DNA Reporters

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 21, 2025

MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression and implicated in various diseases, including cancer. Due to their critical role diagnostics, there is a growing need for sensitive, specific, rapid detection methods miRNAs. In this study, we present dual-accelerated signal amplification platform miRNA biosensing, which integrates spatial confining catalytic hairpin assembly (SC-CHA) with spherical CRISPR/Cas12a (S-CRISPR/Cas12a) system (SC-CHA@S-CRISPR/Cas12a) trans-cleavage of DNA reporters. The method employs biotinylated palindrome-rich sequence (PAS) form nanoballs, serve as scaffold the operation SC-CHA upon binding. products bind crRNA Cas 12a protein, activating S-CRISPR/Cas12a cleave reporter generate detectable fluorescence signal. uniqueness lies combined use nanoballs reporters, both significantly accelerate reaction kinetics, resulting generation. Additionally, nanostructure, integrated system, greatly enhances biostability accelerating kinetics. These features enable exhibit high sensitivity, limit (LOD) low 13.75 fM, excellent specificity, successfully distinguishing miRNA-21 from other assay also biostable, demonstrating reliable performance complex biological samples such human serum. This dual-acceleration approach offers promising solution rapid, specific potential applications early cancer diagnosis clinical monitoring.

Язык: Английский

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Biomaterial-Based CRISPR/Cas9 Delivery Systems for Tumor Treatment

Biomaterials Research, Год журнала: 2024, Номер 28

Опубликована: Янв. 1, 2024

CRISPR/Cas9 gene editing technology is characterized by high specificity and efficiency, has been applied to the treatment of human diseases, especially tumors involving multiple genetic modifications. However, clinical application still faces some major challenges, most urgent which development optimized delivery vectors. Biomaterials are currently best choice for use in vectors owing their tunability, biocompatibility, efficiency. As research on biomaterial continues progress, hope system oncology therapy builds. In this review, we first detail its potential applications tumor therapy. Then, introduce different forms compare physical, viral, non-viral addition, analyze characteristics types We further review recent progress biomaterials as treat specific tumors. Finally, summarize shortcomings prospects biomaterial-based systems.

Язык: Английский

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Harnessing the power of clustered regularly interspaced short palindromic repeats (CRISPR) based microfluidics for next-generation molecular diagnostics

Molecular Biology Reports, Год журнала: 2024, Номер 51(1)

Опубликована: Авг. 8, 2024

Язык: Английский

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CRISPR/Cas12a-enhanced DNA nanomachine for multiple respiratory pathogens detection

Chemical Communications, Год журнала: 2024, Номер 60(99), С. 14814 - 14817

Опубликована: Янв. 1, 2024

We constructed an ICP-MS analysis strategy of a CRISPR/Cas12a-enhanced DNA nanomachine to detect multiple respiratory pathogens.

Язык: Английский

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Development of a Rapid On-Site Detection Method for Largemouth Bass Virus Based on Rpa-Crispr/Cas12a System

Опубликована: Янв. 1, 2025

Язык: Английский

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Antifouling fusion-mediated diagnostic platform to detect viral DNA-positive extracellular vesicles for in situ blood-based liquid biopsy

Biosensors and Bioelectronics, Год журнала: 2025, Номер 286, С. 117568 - 117568

Опубликована: Май 9, 2025

Язык: Английский

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CRISPR-assisted bioanalytical sensing of cytokines

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118318 - 118318

Опубликована: Май 1, 2025

Язык: Английский

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Applications and development trend of biosensors in the detection and diagnosis of gastrointestinal tumors

BioMedical Engineering OnLine, Год журнала: 2025, Номер 24(1)

Опубликована: Июнь 3, 2025

Gastrointestinal malignancies present a significant menace to the well-being of people across globe. Consequently, prompt and precise identification such tumors is utmost importance. Biosensor technology emerges as promising avenue in this regard, possessing considerable potential for detection diagnosis gastrointestinal tumors. This achieved through integration biometric components with physical chemical transducers, enabling rapid, sensitive, specific capabilities. In context colorectal cancer, notable advancements have been made nucleic acids, proteins, cells, exosomes. For instance, threshold miRNA has refined amole level, while methodologies diverse protein markers are under continuous improvement. addition, exosome techniques evolving steadily. Esophageal on other hand, exhibits its own characteristic enzyme cascade immune sensors, among others. Similarly, gastric there numerous innovative approaches miRNA, Looking ahead, performance biosensors tumor anticipated enhance further. The combination multiple joint expected gain prominence, realization miniaturization holds drive improvements treatment tumors, ultimately leading better patient outcomes more effective approach combating these life-threatening diseases.

Язык: Английский

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Establishment of a Lateral Flow Dipstick Detection Method for Influenza A Virus Based on CRISPR/Cas12a System

Deleted Journal, Год журнала: 2024, Номер 6(37), С. 946 - 952

Опубликована: Янв. 1, 2024

This study aimed to develop a rapid, visual PCR-CRISPR/Cas12-LFD method for detecting influenza A by utilizing the conserved region of matrix protein gene.

Язык: Английский

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