3 Biotech, Год журнала: 2024, Номер 15(1)
Опубликована: Дек. 19, 2024
Язык: Английский
Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)
Опубликована: Июль 1, 2024
The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements potential therapeutic applications, focusing primarily on two areas: emerging cell-based therapies promising non-cell modalities. Within context cell therapy, discuss capacity overcome existing translational challenges faced by mainstream therapy paradigms, provide a detailed discussion advantages principal design considerations for boosting efficacy as well list specific examples different disease scenarios. We then explore drug delivery, physical intervention therapies, other areas (e.g., bioadhesives, artificial tissues, biosensors), emphasizing utility beyond mere delivery vehicles. Additionally, complement our latest progress clinical application outline future research directions, particularly terms integration with advanced biomanufacturing This review aims present comprehensive view critical insights into selection both tailored meet requirements diverse diseases situations.
Язык: Английский
Процитировано
83
Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)
Опубликована: Сен. 16, 2023
Abstract Extracellular vesicles (EVs) are 30–150 nm membrane-bound naturally secreted by cells and play important roles in intercellular communication delivering regulatory molecules such as proteins, lipids, nucleic acids metabolites to recipient cells. As natural nano-carriers, EVs possess desirable properties high biocompatibility, biological barrier permeability, low toxicity, immunogenicity, making them potential therapeutic delivery vehicles. derived from specific have inherent targeting capacity towards cell types, which is yet not satisfactory enough for targeted therapy development needs be improved. Surface modifications endow with abilities, significantly improving their efficiency. Herein, we first briefly introduce the biogenesis, composition, uptake function of EVs, review cargo loading approaches EVs. Then, summarize recent advances surface engineering strategies focusing on applications engineered therapy. Altogether, hold great promise various cargos, show promising effects multiple diseases. Graphical
Язык: Английский
Процитировано
56
Materials Today Bio, Год журнала: 2023, Номер 22, С. 100760 - 100760
Опубликована: Авг. 11, 2023
Exosomes have emerged as a promising cell-free therapeutic approach. However, challenges in large-scale production, quality control, and heterogeneity must be overcome before they can used clinically. Biomimetic exosomes containing key components of natural been assembled through extrusion, artificial synthesis, liposome fusion to address these limitations. These exosome-mimetics (EMs) possess similar morphology function but provide higher yields, faster size compared conventional exosomes. This article provides an overview the chemical biological properties various synthetic exosome systems, including nanovesicles (NVs), EMs, hybrid We highlight recent advances production applications nanobiotechnology discuss advantages, limitations, potential clinical programming assembly mimetics.
Язык: Английский
Процитировано
51
Journal of Drug Delivery Science and Technology, Год журнала: 2024, Номер 92, С. 105338 - 105338
Опубликована: Янв. 6, 2024
The rapid advancement of CRISPR-Cas9 technology has instigated a profound transformation in genome editing with significant implications for fields like health, agriculture, and biotechnology. This abstract provides an overview the historical significance fundamental components CRISPR-Cas9, notably Cas9 protein guide RNA, underscoring its pivotal role genetic manipulation. It emphasizes CRISPR-Cas9's preeminence domain precise editing, driving breakthroughs personalized medicine, gene therapy, agriculture. Of paramount importance is integration nanomaterials, encompassing lipid-based polymeric nanoparticles, alongside viral vectors, serving as potent vehicles augmenting delivery efficiency precision. We explore strategies aimed at enhancing through while also addressing ethical regulatory considerations. In expert opinion section, we offer nuanced perspective on present state field, highlighting potential transformative progress research therapy. stands brink unlocking new possibilities providing innovative solutions to address pressing global challenges.
Язык: Английский
Процитировано
39
Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)
Опубликована: Июль 4, 2024
Abstract Messenger RNA (mRNA) has emerged as a promising therapeutic molecule with numerous clinical applications in treating central nervous system disorders, tumors, COVID-19, and other diseases. mRNA therapies must be encapsulated into safe, stable, effective delivery vehicles to preserve the cargo from degradation prevent immunogenicity. Exosomes have gained growing attention because of their good biocompatibility, low immunogenicity, small size, unique capacity traverse physiological barriers, cell-specific tropism. Moreover, these exosomes can engineered utilize natural carriers target specific cells or tissues. This targeted approach will enhance efficacy reduce side effects mRNAs. However, difficulties such lack consistent reliable methods for exosome purification efficient encapsulation large mRNAs addressed. article outlines current breakthroughs cell-derived vesicle-mediated its biomedical applications. Graphical
Язык: Английский
Процитировано
25
Psychiatry and Clinical Neurosciences, Год журнала: 2023, Номер 78(2), С. 83 - 96
Опубликована: Окт. 25, 2023
Extracellular vesicles (EVs) are membrane‐enclosed nanovesicles secreted by cells into the extracellular space and contain functional biomolecules, e.g. signaling receptors, bioactive lipids, nucleic acids, proteins, which can serve as biomarkers. Neurons glial secrete EVs, contributing to various physiological pathological aspects of brain diseases. EVs confer their role in bidirectional crosstalk between central nervous system (CNS) periphery owing distinctive ability cross unique blood–brain barrier (BBB). Thus, blood, cerebrospinal fluid (CSF), urine be intriguing biomarkers, enabling minimally invasive diagnosis CNS Although there has been an enormous interest evaluating promising lack ultra‐sensitive approaches for isolating detecting brain‐derived (BDEVs) hindered development efficient This review presents recent salient findings exosomal focusing on disorders. We summarize highly sensitive sensors EV detection state‐of‐the‐art methods single detection. Finally, prospect developing advanced analysis non‐invasive diseases is presented.
Язык: Английский
Процитировано
30
Cell Death Discovery, Год журнала: 2023, Номер 9(1)
Опубликована: Июль 21, 2023
Abstract The organoids represent one of the greatest revolutions in biomedical field past decade. This three-dimensional (3D) micro-organ cultured vitro has a structure highly similar to that tissue and organ. Using regeneration ability stem cells, 3D organ-like called intestinal is established, which can mimic characteristics real organs, including morphology, function, personalized response specific stimuli. Here, we discuss current cell-based models, understanding molecular pathophysiology, high-throughput screening drugs, drug efficacy testing, toxicological evaluation, organ-based inflammatory bowel disease (IBD). We summarize advances limitations state-of-the-art reconstruction platforms for organoids. challenges, advantages, prospects organs as an model system precision medicine are also discussed.
Язык: Английский
Процитировано
26
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 2241 - 2264
Опубликована: Март 1, 2024
Abstract: Recently, gene therapy has become a subject of considerable research and been widely evaluated in various disease models. Though it is considered as stand-alone agent for COVID-19 vaccination, still suffering from the following drawbacks during its translation bench to bedside: high sensitivity exogenous nucleic acids enzymatic degradation; severe side effects induced either by or components formulation; difficulty cross barriers before reaching therapeutic target. Therefore, successful application therapy, safe reliable transport vector urgently needed. Extracellular vesicles (EVs) are ideal candidate delivery drugs owing their low immunogenicity, good biocompatibility toxicity. To better understand properties EVs advantages drug vehicles, this review covers origin methods generation, well common isolation purification research, with pros cons discussed. Meanwhile, engineering also highlighted. In addition, paper presents progress EVs-mediated microRNAs, small interfering RNAs, messenger plasmids, antisense oligonucleotides. We believe will provide theoretical basis development drugs. Keywords: extracellular vesicles, system,
Язык: Английский
Процитировано
12
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 1363 - 1383
Опубликована: Фев. 1, 2024
Osteoporosis (OP) affects millions of people worldwide, especially postmenopausal women and the elderly. Although current available anti-OP agents can show promise in slowing down bone resorption, most are not specifically delivered to hard tissue, causing significant toxicity. A bone-targeted nanodrug delivery system reduce side effects precisely deliver drug candidates bone. This review focuses on progress nanoparticles OP therapy. We enumerate existing medications, types categorize pairs common bone-targeting functional groups. Finally, we summarize potential use treatment. Ongoing research into development targeted ligands nanocarriers will continue expand possibilities OP-targeted therapies clinical application.
Язык: Английский
Процитировано
11
Macromolecular Rapid Communications, Год журнала: 2024, Номер 45(11)
Опубликована: Фев. 24, 2024
Abstract Hydrogels, key in biomedical research for their hydrophilicity and versatility, have evolved with hydrogel microspheres (HMs) of micron‐scale dimensions, enhancing role minimally invasive therapeutic delivery, tissue repair, regeneration. The recent emergence nanomaterials has ushered a revolutionary transformation the field, which demonstrates tremendous potential targeted therapies, biological imaging, disease diagnostics. Consequently, integration advanced nanotechnology promises to trigger new revolution realm hydrogels. HMs loaded combine advantages both hydrogels nanomaterials, enables multifaceted functionalities such as efficient drug sustained release, therapy, lubrication, biochemical detection, medical biosensing monitoring, micro‐robotics. Here, this review comprehensively expounds upon commonly used classifications. Then, it provides comprehensive insights into raw materials preparation methods HMs. Besides, common strategies employed achieve nano‐micron combinations are summarized, latest applications these combined field elucidated. Finally, valuable future design development provided.
Язык: Английский
Процитировано
10