Improving the use of CRISPR/Cas9 gene editing machinery as a cancer therapeutic tool with the help of nanomedicine

3 Biotech, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 19, 2024

Язык: Английский

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Exosome-Based Drug Delivery Systems

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Extracellular vesicles, especially exosomes, have attracted widespread attention in the biomedical field recent years. They a unique ability to efficiently transport variety of bioactive molecules, property that makes them show great potential precision medicine. In addition, exosomes can evade detection by immune system, providing new solution for drug delivery and cancer research. This manuscript provides an overview exosome biogenesis, isolation-related techniques, their application as therapeutic vehicles. We discuss various strategies loading exosomal cargo engineering targeted delivery, highlighting advances exosome-based vaccines personalized therapies. book chapter concludes emphasizing transformative impact therapeutics on medicine, outlining future direction this its overcome traditional limitations.

Язык: Английский

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A Semiconducting Polymer NanoCRISPR for Near-Infrared Photoactivatable Gene Editing and Cancer Gene Therapy

Nano Letters, Год журнала: 2025, Номер unknown

Опубликована: Март 7, 2025

Clustered regularly interspaced short palindromic repeat (CRISPR) gene editing has poor efficacy and off-target side effect concerns. We herein report a semiconducting polymer (SP)-based nanoCRISPR system to improve CRISPR delivery allow for near-infrared (NIR) photoactivatable cancer therapy. An amphiphilic SP acts as photothermal converter, its backbone is grafted with single-stranded deoxyribonucleic acid (DNA), which enables hybridization single guide ribonucleic (sgRNA) via complementary base pairing form sgRNA/SP-DNA. This sgRNA/SP-DNA nanosystem (nanoCRISPR) can effectively deliver sgRNA into cells generate heat under NIR laser irradiation the effect. The localized triggers dissociation of DNA control release sgRNA, thereby achieving precise regulation activity. technology able precisely regulate expression green fluorescent protein (GFP) polo-like kinase 1 (PLK1) precision

Язык: Английский

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Redefining Hepatocellular Carcinoma Treatment: Nanotechnology Meets Tumor Immune Microenvironment

Journal of drug targeting, Год журнала: 2025, Номер unknown, С. 1 - 47

Опубликована: Март 13, 2025

Hepatocellular carcinoma (HCC) is one of the most lethal malignancies worldwide, characterized by its complex pathogenesis and poor therapeutic outcomes. Despite recent advances in targeted molecular therapies, immune checkpoint inhibitors (ICIs), radiotherapy, conventional chemotherapy, five-year survival rate for this neoplasm remains dismally low. The progress nanotechnology has revolutionized cancer treatment years. These provide unprecedented opportunities to overcome current limitations different modalities. This review provides a comprehensive analysis how interfaces with tumor microenvironment (TIME) HCC can present new frontier interventions HCC. We critically overview latest developments nanoparticle-based delivery systems various drugs also other antitumor agents like thermal therapy radiotherapy. highlight unique ability nanoparticles modulate immunosuppressive (TME) enhance efficacy. Furthermore, we analyze emerging strategies that exploit nanoformulations biological barriers drug bioavailability treatment.

Язык: Английский

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Plasmid DNA and mRNA delivery: Approaches and challenges

Advances in immunology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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