ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
17(1), С. 725 - 738
Опубликована: Дек. 16, 2024
Targeting
tumor
markers
is
one
of
the
most
important
approaches
to
therapy,
and
"suicide"
pattern
marker
response
a
very
challenging
study.
Telomerase,
as
key
factors
associated
with
human
longevity
cancer
progression,
considered
be
an
emerging
biomarker
for
diagnosis.
The
targeted
drug
delivery
nanobomb─BIBR1532@HSN/FQDNA/MUC1
aptamer
(B@HDA)
prepared
in
this
study
based
on
hollow
silica
nanoparticles
(HSN)
CRISPR
systems.
Amino-modified
FQDNA
amino-modified
MUC1
are
covalently
attached
surface
carboxyl-functionalized
HSN.
modified
directs
nanobomb
specifically
target
breast
cells
(MCF-7)
sequesters
telomerase
inhibitor
(BIBR1532)
within
Telomerase
primers
(TPs)
recognized
by
highly
expressed
MCF-7
elongated
form
DNA
substrates.
substrate
pairs
crRNA
bases
effectively
activate
CRISPR-Cas12a.
activated
CRISPR-Cas12a
precisely
cut
FQDNA,
releasing
BIBR1532,
which
inhibits
activity.
This
strategy
achieves
"suicide".
described
above
has
following
advantages.
(1)
"closing"
effect
contributes
reducing
nonspecific
release
BIBR1532.
(2)
B@HDA,
combined
CRISPR,
regulates
mitochondrial
dysfunction
cell
senescence
cells.
(3)
In
tumor-bearing
mouse
model,
exhibits
good
biocompatibility
obvious
ablation
tumors,
suggesting
potential
application
prospects
across
wide
range
lines.
summary,
proposed
provides
tunable
switch
approach
specific
inhibition
reduction
growth,
representing
promising
avenue
promoting
treating
cancer.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Окт. 8, 2024
Liquid
biopsy
is
a
minimally
invasive
method
that
uses
biofluid
samples
instead
of
tissue
for
cancer
diagnosis.
Exosomes
are
small
extracellular
vesicles
secreted
by
donor
cells
and
act
as
mediators
intercellular
communication
in
human
health
disease.
Due
to
their
important
roles,
exosomes
have
been
considered
promising
biomarkers
liquid
biopsy.
However,
traditional
methods
exosome
isolation
cargo
detection
time-consuming
inefficient,
limiting
practical
application.
In
the
past
decades,
many
new
strategies,
such
microfluidic
chips,
nanowire
arrays
electrochemical
biosensors,
proposed
achieve
rapid,
accurate
high-throughput
analysis
exosomes.
this
review,
we
discussed
about
advance
exosome-based
technology,
including
isolation,
enrichment,
approaches.
The
comparison
currently
available
also
included.
Finally,
summarized
advantages
limitations
present
strategies
further
gave
perspective
future
translational
use.
Epigenetics & Chromatin,
Год журнала:
2024,
Номер
17(1)
Опубликована: Окт. 16, 2024
Cancer
has
arisen
from
both
genetic
mutations
and
epigenetic
changes,
making
epigenetics
a
crucial
area
of
research
for
innovative
cancer
prevention
treatment
strategies.
This
dual
perspective
propelled
into
the
forefront
research.
review
highlights
important
roles
DNA
methylation,
histone
modifications
non-coding
RNAs
(ncRNAs),
particularly
microRNAs
(miRNAs)
long
RNAs,
which
are
key
regulators
cancer-related
gene
expression.
It
explores
potential
epigenetic-based
therapies
to
revolutionize
patient
outcomes
by
selectively
modulating
specific
markers
involved
in
tumorigenesis.
The
examines
promising
biomarkers
early
detection
prognosis.
also
recent
progress
oligonucleotide-based
therapies,
including
antisense
oligonucleotides
(ASOs)
antimiRs,
precisely
modulate
processes.
Furthermore,
concept
editing
is
discussed,
providing
insight
future
role
precision
medicine
patients.
integration
nanomedicine
therapy
been
explored
offers
approaches
improve
therapeutic
efficacy.
comprehensive
advances
seeks
advance
field
oncology,
ultimately
culminating
improved
fight
against
cancer.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(25), С. 9654 - 9662
Опубликована: Июнь 12, 2023
Triple-negative
breast
cancer
is
particularly
difficult
to
treat
because
of
its
high
degree
malignancy
and
poor
prognosis.
A
fluorescence
resonance
energy
transfer
(FRET)
nanoplatform
plays
a
very
important
role
in
disease
diagnosis
treatment
due
unique
detection
performance.
Combining
the
properties
agglomeration-induced
emission
fluorophore
FRET
pair,
nanoprobe
(HMSN/DOX/RVRR/PAMAM/TPE)
induced
by
specific
cleavage
was
designed.
First,
hollow
mesoporous
silica
nanoparticles
(HMSNs)
were
used
as
drug
carriers
load
doxorubicin
(DOX).
HMSN
nanopores
coated
with
RVRR
peptide.
Then,
polyamylamine/phenylethane
(PAMAM/TPE)
combined
outermost
layer.
When
Furin
cut
off
peptide,
DOX
released
adhered
PAMAM/TPE.
Finally,
TPE/DOX
pair
constituted.
The
overexpression
triple-negative
cell
line
(MDA-MB-468
cell)
can
be
quantitatively
detected
signal
generation,
so
monitor
physiology.
In
conclusion,
HMSN/DOX/RVRR/PAMAM/TPE
nanoprobes
designed
provide
new
idea
for
quantitative
delivery,
which
conducive
early
cancer.
Chemical Communications,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Extracellular
vesicles
have
been
deemed
as
potential
drug
carriers
for
treatment
of
various
diseases.
Recent
advances
summarized,
including
the
sources,
delivery
function,
extraction
and
cargo-loading
technology
extracellular
vesicles.
Chemical Communications,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
CZPA
NC,
which
targets
cell
and
mitochondrial
function,
can
increase
intracellular
hypoxia
activate
a
drug
to
produce
radicals,
causing
DNA
damage,
dysfunction,
changes
in
morphology.
The Analyst,
Год журнала:
2024,
Номер
149(4), С. 1250 - 1261
Опубликована: Янв. 1, 2024
An
all-in-one
detection
strategy
was
proposed
for
the
identification
of
breast
cancer-derived
exosomal
miRNA-21,
exhibiting
high
sensitivity
and
specificity
on
a
pen-based
paper
chip.
Extracellular
vesicles
(EVs)
are
emerging
biomarkers
in
liquid
biopsy
that
have
gained
increasing
attention
disease
diagnosis
and
prognosis
monitoring.
Most
reported
detection
methods
require
the
isolation
of
EVs
from
complex
body
liquids,
often
involving
multiple
washing
steps
to
remove
excess
reagents
eliminate
background
interference.
Nonetheless,
these
not
only
cause
loss
but
also
result
poor
repeatability
prolonged
duration.
The
focus
on
wash-free
is
due
specific
ability
avoid
removal
surplus
and,
some
cases,
even
purification
EVs.
Viewing
different
methodological
perspectives,
this
review
summarizes
recent
advances
EVs,
containing
aggregation
induction,
proximity
sensing,
allosteric
probes,
phase
separation,
Roman
spectroscopy,
field-effect
transistor
microcantilever.
pros
cons
each
strategy
impartially
evaluated
concludes
prospects
for
future
developments
field.
