ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
17(1), С. 725 - 738
Опубликована: Дек. 16, 2024
Targeting
tumor
markers
is
one
of
the
most
important
approaches
to
therapy,
and
"suicide"
pattern
marker
response
a
very
challenging
study.
Telomerase,
as
key
factors
associated
with
human
longevity
cancer
progression,
considered
be
an
emerging
biomarker
for
diagnosis.
The
targeted
drug
delivery
nanobomb─BIBR1532@HSN/FQDNA/MUC1
aptamer
(B@HDA)
prepared
in
this
study
based
on
hollow
silica
nanoparticles
(HSN)
CRISPR
systems.
Amino-modified
FQDNA
amino-modified
MUC1
are
covalently
attached
surface
carboxyl-functionalized
HSN.
modified
directs
nanobomb
specifically
target
breast
cells
(MCF-7)
sequesters
telomerase
inhibitor
(BIBR1532)
within
Telomerase
primers
(TPs)
recognized
by
highly
expressed
MCF-7
elongated
form
DNA
substrates.
substrate
pairs
crRNA
bases
effectively
activate
CRISPR-Cas12a.
activated
CRISPR-Cas12a
precisely
cut
FQDNA,
releasing
BIBR1532,
which
inhibits
activity.
This
strategy
achieves
"suicide".
described
above
has
following
advantages.
(1)
"closing"
effect
contributes
reducing
nonspecific
release
BIBR1532.
(2)
B@HDA,
combined
CRISPR,
regulates
mitochondrial
dysfunction
cell
senescence
cells.
(3)
In
tumor-bearing
mouse
model,
exhibits
good
biocompatibility
obvious
ablation
tumors,
suggesting
potential
application
prospects
across
wide
range
lines.
summary,
proposed
provides
tunable
switch
approach
specific
inhibition
reduction
growth,
representing
promising
avenue
promoting
treating
cancer.
Biosensors,
Год журнала:
2023,
Номер
13(11), С. 968 - 968
Опубликована: Ноя. 6, 2023
A
powerful
and
accurate
method
for
identifying
isolating
cells
would
be
of
great
importance
due
to
its
sensitivity,
gentleness
effectiveness.
Here,
we
designed
a
receptor-based
DNA
logic
device
that
allows
Boolean
analysis
multiple
cells.
For
ease
expression,
the
molecules
on
cell
surface
can
bind
aptamer
are
referred
as
"receptors".
This
sends
signals
based
sgc8c
sgc4f
receptor
expression
by
performing
NOT,
NOR,
AND
OR
operations,
amplifies
evaluates
using
HCR.
Meanwhile,
release
ICG
from
endopore
HMSNs
is
controlled
affecting
structural
changes
in
device.
approach
accurately
identify
treat
demand
presence
or
absence
cell-specific
receptors,
facilitating
development
personalized
medicine.
Advanced NanoBiomed Research,
Год журнала:
2023,
Номер
4(1)
Опубликована: Дек. 3, 2023
Liquid
biopsy
has
received
increasing
attention
as
a
new
disease
detection
modality
because
of
its
noninvasive,
simple
sampling,
and
reproducible
assay
advantages.
Among
the
markers
liquid
biopsy,
extracellular
vesicles
(EVs)
are
considered
promising
biomarkers
they
contain
large
amount
biological
information
have
significant
role
in
physiological
activities.
The
emergence
progression
some
these
diseases
associated
with
miRNAs
carried
by
EVs
(EV‐miRNAs).
Therefore,
high‐sensitive
EV‐miRNAs
is
essential
clinical
applications.
A
growing
number
strategies,
including
biosensors,
situ
methods,
microfluidics
been
developed
for
EV‐miRNA
applied
diagnosis
such
cancer.
This
review
summarizes
probes,
signal
amplification,
methods
detection,
well
application
membrane
fusion‐based
integrated
microfluidic
chips
detection.
challenges
materials
techniques
diagnostic
applications
also
discussed.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
17(1), С. 725 - 738
Опубликована: Дек. 16, 2024
Targeting
tumor
markers
is
one
of
the
most
important
approaches
to
therapy,
and
"suicide"
pattern
marker
response
a
very
challenging
study.
Telomerase,
as
key
factors
associated
with
human
longevity
cancer
progression,
considered
be
an
emerging
biomarker
for
diagnosis.
The
targeted
drug
delivery
nanobomb─BIBR1532@HSN/FQDNA/MUC1
aptamer
(B@HDA)
prepared
in
this
study
based
on
hollow
silica
nanoparticles
(HSN)
CRISPR
systems.
Amino-modified
FQDNA
amino-modified
MUC1
are
covalently
attached
surface
carboxyl-functionalized
HSN.
modified
directs
nanobomb
specifically
target
breast
cells
(MCF-7)
sequesters
telomerase
inhibitor
(BIBR1532)
within
Telomerase
primers
(TPs)
recognized
by
highly
expressed
MCF-7
elongated
form
DNA
substrates.
substrate
pairs
crRNA
bases
effectively
activate
CRISPR-Cas12a.
activated
CRISPR-Cas12a
precisely
cut
FQDNA,
releasing
BIBR1532,
which
inhibits
activity.
This
strategy
achieves
"suicide".
described
above
has
following
advantages.
(1)
"closing"
effect
contributes
reducing
nonspecific
release
BIBR1532.
(2)
B@HDA,
combined
CRISPR,
regulates
mitochondrial
dysfunction
cell
senescence
cells.
(3)
In
tumor-bearing
mouse
model,
exhibits
good
biocompatibility
obvious
ablation
tumors,
suggesting
potential
application
prospects
across
wide
range
lines.
summary,
proposed
provides
tunable
switch
approach
specific
inhibition
reduction
growth,
representing
promising
avenue
promoting
treating
cancer.
