Isoferulic acid facilitates effective clearance of hypervirulent Klebsiella pneumoniae through targeting capsule
PLoS Pathogens,
Год журнала:
2025,
Номер
21(1), С. e1012787 - e1012787
Опубликована: Янв. 6, 2025
Hypervirulent
Klebsiella
pneumoniae
(hvKP)
poses
an
alarming
threat
in
clinical
settings
and
global
public
health
owing
to
its
high
pathogenicity,
epidemic
success
rapid
development
of
drug
resistance,
especially
the
emergence
carbapenem-resistant
lineages
(CR-hvKP).
With
decline
“last
resort”
antibiotic
class
decreasing
efficacy
first-line
antibiotics,
innovative
alternative
therapeutics
are
urgently
needed.
Capsule,
essential
virulence
determinant,
is
a
major
cause
enhanced
pathogenicity
hvKP
thus
represents
attractive
target
prevent
devastating
outcomes
caused
by
infection.
Here,
we
identified
isoferulic
acid
(IFA),
natural
phenolic
compound
widely
present
traditional
herbal
medicines,
as
potent
broad-spectrum
K
.
capsule
inhibitor
that
suppresses
polysaccharide
synthesis
increasing
energy
status
bacteria.
In
this
way,
IFA
remarkably
reduced
thickness
impaired
hypercapsule-associated
hypermucoviscosity
phenotype
(HMV),
thereby
significantly
sensitizing
complement-mediated
bacterial
killing
accelerating
host
cell
adhesion
phagocytosis.
Consequently,
facilitated
effective
clearance
protected
mice
from
lethal
infection,
evidenced
limited
dissemination
significant
improvement
survival
rate.
conclusion,
work
promotes
capsule-targeted
therapeutic
strategy
for
use
promising
candidate
intervention
curb
particularly
drug-resistant
cases.
Язык: Английский
A new approach to overcoming antibiotic-resistant bacteria: Traditional Chinese medicine therapy based on the gut microbiota
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2023,
Номер
13
Опубликована: Апрель 6, 2023
With
the
irrational
use
of
antibiotics
and
increasing
abuse
oral
antibiotics,
drug
resistance
gastrointestinal
pathogens
has
become
a
prominent
problem
in
clinical
practice.
Gut
microbiota
plays
an
important
role
maintaining
human
health,
change
also
affects
activity
pathogenic
bacteria.
Interfering
with
antibiotic
resistant
bacteria
by
affecting
gut
regulatory
signal.
In
application,
due
to
unique
advantages
traditional
Chinese
medicine
sterilization
resistance,
it
is
possible
for
improve
microbial
microenvironment.
This
review
discusses
strategies
treatment
drug-resistant
bacterial
infections
changing
microenvironment,
unlocking
interaction
between
Язык: Английский
Application of inhibitors targeting the type III secretion system in phytopathogenic bacteria
Lu-Lu He,
Lan-Tu Xiong,
Xin Wang
и другие.
Chinese Chemical Letters,
Год журнала:
2024,
Номер
unknown, С. 110044 - 110044
Опубликована: Май 1, 2024
Язык: Английский
In vitro and in vivo activities of scutellarein, a novel polyphosphate kinase 1 inhibitor against Acinetobacter baumannii infection
Microbial Cell Factories,
Год журнала:
2024,
Номер
23(1)
Опубликована: Окт. 8, 2024
Inorganic
polyphosphate
(polyP)-targeted
kinase
1
(PPK1)
has
attracted
much
attention
by
virtue
of
its
importance
in
bacterial
pathogenicity
and
persistence,
as
well
exclusive
presence
microorganisms.
However,
only
very
few
drugs
have
been
found
to
be
efficacious
inhibiting
the
Acinetobacter
baumannii
(A.
baumannii)
PPK1
protein.
Язык: Английский
Fisetin inhibits Salmonella Typhimurium type III secretion system regulator HilD and reduces pathology in vivo
Microbiology Spectrum,
Год журнала:
2023,
Номер
12(1)
Опубликована: Дек. 11, 2023
Salmonella
spp.
remains
a
major
worldwide
health
concern
that
causes
significant
morbidity
and
mortality
in
both
humans
animals.
The
spread
of
antimicrobial
resistant
strains
has
declined
the
efficacy
conventional
chemotherapy.
Thus,
novel
anti-infection
drugs
or
strategies
are
needed.
Anti-virulence
strategy
represents
one
promising
means
for
treatment
bacterial
infections.
In
this
study,
we
found
natural
compound
fisetin
could
inhibit
invasion
host
cells
by
targeting
SPI-1
regulation.
Fisetin
impaired
interaction
regulatory
protein
HilD
with
promoters
its
target
genes,
thereby
suppressing
expression
T3SS-1
effectors
as
well
structural
proteins.
Moreover,
reduce
pathology
murine
infection
model.
Collectively,
our
results
suggest
may
serve
lead
development
anti-Salmonella
drugs.
Язык: Английский
Investigation of the inhibitory effect and mechanism of epigallocatechin-3-gallate against Streptococcus suis sortase A
Journal of Applied Microbiology,
Год журнала:
2023,
Номер
134(9)
Опубликована: Авг. 26, 2023
Streptococcus
suis
seriously
harms
people
and
animals,
importantly,
causes
great
economic
losses
in
the
pig
industry.
Similar
to
most
Gram-positive
pathogenic
bacteria,
sortase
A
(SrtA)
of
S.
can
mediate
anchoring
a
variety
virulence
factors
that
contain
specific
sorting
sequences
surface
bacterial
cell
wall
envelope
participate
pathogenicity.
The
purpose
this
study
is
clarify
molecular
mechanism
epigallocatechin-3-gallate
(EGCG)
inhibiting
SrtA
provide
more
evidence
for
development
novel
anti-S.
infections
drugs.Through
substrate
cleavage
experiment,
we
found
main
component
green
tea,
EGCG,
effectively
inhibit
enzyme
activity
SrtA.
Further,
docking
dynamics
simulation
were
used
its
inhibitory
effect,
demonstrating
EGCG
mainly
interacts
with
amino
acids
at
113
115
exert
function.
It
was
previously
growth
reduce
suilysin
expression.
Our
research
reveals
new
function
infection.Our
proves
transpeptidase
We
also
accompanying
mechanism,
providing
sufficient
use
as
potential
lead
compound
against
infection.
Язык: Английский