Investigation of the inhibitory effect and mechanism of epigallocatechin-3-gallate against Streptococcus suis sortase A DOI
Fei Lou, Hui Huang, Yaping Li

et al.

Journal of Applied Microbiology, Journal Year: 2023, Volume and Issue: 134(9)

Published: Aug. 26, 2023

Streptococcus suis seriously harms people and animals, importantly, causes great economic losses in the pig industry. Similar to most Gram-positive pathogenic bacteria, sortase A (SrtA) of S. can mediate anchoring a variety virulence factors that contain specific sorting sequences surface bacterial cell wall envelope participate pathogenicity. The purpose this study is clarify molecular mechanism epigallocatechin-3-gallate (EGCG) inhibiting SrtA provide more evidence for development novel anti-S. infections drugs.Through substrate cleavage experiment, we found main component green tea, EGCG, effectively inhibit enzyme activity SrtA. Further, docking dynamics simulation were used its inhibitory effect, demonstrating EGCG mainly interacts with amino acids at 113 115 exert function. It was previously growth reduce suilysin expression. Our research reveals new function infection.Our proves transpeptidase We also accompanying mechanism, providing sufficient use as potential lead compound against infection.

Language: Английский

Isoferulic acid facilitates effective clearance of hypervirulent Klebsiella pneumoniae through targeting capsule DOI Creative Commons
Tingting Wang,

Huaizhi Yang,

Qiushuang Sheng

et al.

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(1), P. e1012787 - e1012787

Published: Jan. 6, 2025

Hypervirulent Klebsiella pneumoniae (hvKP) poses an alarming threat in clinical settings and global public health owing to its high pathogenicity, epidemic success rapid development of drug resistance, especially the emergence carbapenem-resistant lineages (CR-hvKP). With decline “last resort” antibiotic class decreasing efficacy first-line antibiotics, innovative alternative therapeutics are urgently needed. Capsule, essential virulence determinant, is a major cause enhanced pathogenicity hvKP thus represents attractive target prevent devastating outcomes caused by infection. Here, we identified isoferulic acid (IFA), natural phenolic compound widely present traditional herbal medicines, as potent broad-spectrum K . capsule inhibitor that suppresses polysaccharide synthesis increasing energy status bacteria. In this way, IFA remarkably reduced thickness impaired hypercapsule-associated hypermucoviscosity phenotype (HMV), thereby significantly sensitizing complement-mediated bacterial killing accelerating host cell adhesion phagocytosis. Consequently, facilitated effective clearance protected mice from lethal infection, evidenced limited dissemination significant improvement survival rate. conclusion, work promotes capsule-targeted therapeutic strategy for use promising candidate intervention curb particularly drug-resistant cases.

Language: Английский

Citations

0
A new approach to overcoming antibiotic-resistant bacteria: Traditional Chinese medicine therapy based on the gut microbiota DOI Creative Commons
Peng Xue, Rui Sang, Nan Li

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: April 6, 2023

With the irrational use of antibiotics and increasing abuse oral antibiotics, drug resistance gastrointestinal pathogens has become a prominent problem in clinical practice. Gut microbiota plays an important role maintaining human health, change also affects activity pathogenic bacteria. Interfering with antibiotic resistant bacteria by affecting gut regulatory signal. In application, due to unique advantages traditional Chinese medicine sterilization resistance, it is possible for improve microbial microenvironment. This review discusses strategies treatment drug-resistant bacterial infections changing microenvironment, unlocking interaction between

Language: Английский

Citations

8
Application of inhibitors targeting the type III secretion system in phytopathogenic bacteria DOI

Lu-Lu He,

Lan-Tu Xiong,

Xin Wang

et al.

Chinese Chemical Letters, Journal Year: 2024, Volume and Issue: unknown, P. 110044 - 110044

Published: May 1, 2024

Language: Английский

Citations

2
In vitro and in vivo activities of scutellarein, a novel polyphosphate kinase 1 inhibitor against Acinetobacter baumannii infection DOI Creative Commons
Yuping Song, Hongfa Lv, Lei Xu

et al.

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: Oct. 8, 2024

Inorganic polyphosphate (polyP)-targeted kinase 1 (PPK1) has attracted much attention by virtue of its importance in bacterial pathogenicity and persistence, as well exclusive presence microorganisms. However, only very few drugs have been found to be efficacious inhibiting the Acinetobacter baumannii (A. baumannii) PPK1 protein.

Language: Английский

Citations

2
Fisetin inhibits Salmonella Typhimurium type III secretion system regulator HilD and reduces pathology in vivo DOI Creative Commons
Siqi Li, Hongtao Liu,

Jingyan Shu

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 12(1)

Published: Dec. 11, 2023

Salmonella spp. remains a major worldwide health concern that causes significant morbidity and mortality in both humans animals. The spread of antimicrobial resistant strains has declined the efficacy conventional chemotherapy. Thus, novel anti-infection drugs or strategies are needed. Anti-virulence strategy represents one promising means for treatment bacterial infections. In this study, we found natural compound fisetin could inhibit invasion host cells by targeting SPI-1 regulation. Fisetin impaired interaction regulatory protein HilD with promoters its target genes, thereby suppressing expression T3SS-1 effectors as well structural proteins. Moreover, reduce pathology murine infection model. Collectively, our results suggest may serve lead development anti-Salmonella drugs.

Language: Английский

Citations

3
Investigation of the inhibitory effect and mechanism of epigallocatechin-3-gallate against Streptococcus suis sortase A DOI
Fei Lou, Hui Huang, Yaping Li

et al.

Journal of Applied Microbiology, Journal Year: 2023, Volume and Issue: 134(9)

Published: Aug. 26, 2023

Streptococcus suis seriously harms people and animals, importantly, causes great economic losses in the pig industry. Similar to most Gram-positive pathogenic bacteria, sortase A (SrtA) of S. can mediate anchoring a variety virulence factors that contain specific sorting sequences surface bacterial cell wall envelope participate pathogenicity. The purpose this study is clarify molecular mechanism epigallocatechin-3-gallate (EGCG) inhibiting SrtA provide more evidence for development novel anti-S. infections drugs.Through substrate cleavage experiment, we found main component green tea, EGCG, effectively inhibit enzyme activity SrtA. Further, docking dynamics simulation were used its inhibitory effect, demonstrating EGCG mainly interacts with amino acids at 113 115 exert function. It was previously growth reduce suilysin expression. Our research reveals new function infection.Our proves transpeptidase We also accompanying mechanism, providing sufficient use as potential lead compound against infection.

Language: Английский

Citations

1