ABSTRACT
The
bacteria
living
in
the
human
gut
are
essential
for
host
health.
Though
composition
and
metabolism
of
these
well
described
both
healthy
hosts
those
with
intestinal
disease,
less
is
known
about
metabolic
activity
prior
to,
during,
disease
development—especially
regarding
bacterial
replication.
Here,
we
use
a
recently
developed
single-cell
technique
alongside
existing
metagenomics-based
tools
to
identify,
track,
quantify
replicating
ex
vivo
situ
dextran
sodium
sulfate
(DSS)
mouse
model
colitis.
We
show
that
proportion
decreases
when
mice
have
highest
levels
inflammation
returns
baseline
as
begin
recovering.
In
addition,
report
significant
alterations
community
during
colitis
development.
On
taxa
level,
observe
changes
abundance
such
mucus-degrading
Akkermansia
poorly
Erysipelatoclostridium
genus.
further
demonstrate
many
exhibit
variable
replication
rates
colitis,
including
muciniphila
.
Lastly,
development
positively
correlated
increases
presence
which
predicted
be
fast
replicators.
This
could
suggest
potential
replicate
quickly
may
an
advantage
inflammation.
These
data
support
need
additional
research
using
activity-based
approaches
characterize
response
its
consequences
microbial
community.
IMPORTANCE
It
inside
important
Indeed,
type
present
their
different
people
versus
disease.
However,
how
replicating,
especially
someone
begins
develop
particularly
it
thought
metabolically
active
more
relevant
address
this
gap
several
complementary
reveal
quickly,
recover
from
work
can
serve
future
identify
actively
growing
impacting
health,
or
why
particular
tend
thrive
Food & Function,
Год журнала:
2023,
Номер
15(2), С. 516 - 529
Опубликована: Дек. 11, 2023
The
structure
of
soluble
dietary
fiber
unfermented
wheat
bran
(UFSDF)
and
fermented
Isaria
cicadae
Miq
.
(FSDF)
their
effects
on
mice
with
colitis
were
studied.
Results
showed
that
FSDF
had
better
anti-inflammatory
effect.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 1, 2024
Abstract
The
bacteria
living
in
the
human
gut
are
essential
for
host
health.
Though
composition
and
metabolism
of
these
is
well
described
both
healthy
hosts
those
with
intestinal
disease,
less
known
about
activity
prior
to,
during,
disease
development
–
especially
regarding
bacterial
replication.
Here,
we
use
a
recently
developed
single-cell
technique
alongside
existing
metagenomics-based
tools
to
identify,
track,
quantify
replicating
their
replication
dynamics
dextran
sodium
sulfate
mouse
model
colitis.
We
show
that
proportion
decreases
when
mice
have
highest
levels
inflammation
returns
baseline
as
begin
recovering.
additionally
report
significant
alterations
total
community
during
colitis
development.
On
taxa
level,
observe
changes
abundance
such
mucus-degrading
Akkermansia
muciniphila
poorly
Erysipelatoclostridium
genus.
further
demonstrate
many
exhibit
variable
rates
colitis,
including
A.
.
Lastly,
positively
correlated
increases
presence
predicted
be
fast
replicators,
suggesting
potential
replicate
quickly
may
an
advantage
inflammation.
These
data
support
need
additional
research
using
activity-based
approaches
characterize
response
its
consequences
microbial
at
large.
Importance
It
inside
important
Indeed,
type
which
present
different
people
versus
disease.
However,
how
replicating,
someone
begins
develop
This
it
thought
active
more
relevant
addressing
this
gap
by
several
complementary
reveal
quickly,
recover
from
work
can
serve
future
identify
impacting
health,
or
why
particular
tend
thrive
ABSTRACT
The
bacteria
living
in
the
human
gut
are
essential
for
host
health.
Though
composition
and
metabolism
of
these
well
described
both
healthy
hosts
those
with
intestinal
disease,
less
is
known
about
metabolic
activity
prior
to,
during,
disease
development—especially
regarding
bacterial
replication.
Here,
we
use
a
recently
developed
single-cell
technique
alongside
existing
metagenomics-based
tools
to
identify,
track,
quantify
replicating
ex
vivo
situ
dextran
sodium
sulfate
(DSS)
mouse
model
colitis.
We
show
that
proportion
decreases
when
mice
have
highest
levels
inflammation
returns
baseline
as
begin
recovering.
In
addition,
report
significant
alterations
community
during
colitis
development.
On
taxa
level,
observe
changes
abundance
such
mucus-degrading
Akkermansia
poorly
Erysipelatoclostridium
genus.
further
demonstrate
many
exhibit
variable
replication
rates
colitis,
including
muciniphila
.
Lastly,
development
positively
correlated
increases
presence
which
predicted
be
fast
replicators.
This
could
suggest
potential
replicate
quickly
may
an
advantage
inflammation.
These
data
support
need
additional
research
using
activity-based
approaches
characterize
response
its
consequences
microbial
community.
IMPORTANCE
It
inside
important
Indeed,
type
present
their
different
people
versus
disease.
However,
how
replicating,
especially
someone
begins
develop
particularly
it
thought
metabolically
active
more
relevant
address
this
gap
several
complementary
reveal
quickly,
recover
from
work
can
serve
future
identify
actively
growing
impacting
health,
or
why
particular
tend
thrive
