Phage display based biosensing: Recent advances and challenges

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 173, С. 117629 - 117629

Опубликована: Март 5, 2024

Язык: Английский

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Sensitive Lateral Flow Immunoassay Based on Specific Peptide and Superior Oxidase Mimics with a Universal Dual-Mode Significant Signal Amplification

Analytical Chemistry, Год журнала: 2023, Номер 95(33), С. 12532 - 12540

Опубликована: Авг. 8, 2023

Rapid and sensitive antigen detection using a lateral flow immunoassay (LFIA) is crucial for diagnosing infectious diseases due to its simplicity, speed, user-friendly features. However, it remains critical issue explore specific biorecognition elements powerful signal amplification. In this study, taking SARS-CoV-2 as proof of concept, peptide, WFLNDSELIML, binding the spike (S) was identified by nonamplified biopanning method, which exhibited high affinity target, with dissociation constant 9.29 ± 1.55 nM. Molecular docking analysis reveals that peptide binds N-terminal domain S antigen. Then, capture probe angiotensin-converting enzyme 2 probe, peptide-based (pLFIA) without any antibody developed, polydopamine nanosphere (PDA)@MnO2 nanocomposite excellent oxidase-like activity used colorimetric label, exhibiting dual-mode remarkable amplification natural melanin on-demand nanozyme catalytic enhancement. The PDA@MnO2-based pLFIA capable detecting limit 8.01 pg/mL, 18.7 times lower than conventional tagged gold nanoparticles. Additionally, as-proposed can detect up 150 transduction units/mL pseudoviruses spiked in saliva samples. Given outstanding analytical performance, proposed may offer reliable option rapid diagnosis SARS-CoV-2.

Язык: Английский

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One-pot synthesized Au@Pt nanostars-based lateral flow immunoassay for colorimetric and photothermal dual-mode detection of SARS-CoV-2 nucleocapsid antibody

Analytica Chimica Acta, Год журнала: 2024, Номер 1292, С. 342241 - 342241

Опубликована: Янв. 11, 2024

Язык: Английский

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Smart and emerging point of care electrochemical sensors based on nanomaterials for SARS-CoV-2 virus detection: Towards designing a future rapid diagnostic tool

Chemosphere, Год журнала: 2024, Номер 352, С. 141269 - 141269

Опубликована: Янв. 31, 2024

Язык: Английский

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Net-Shaped DNA Nanostructure-Based Lateral Flow Assays for Rapid and Sensitive SARS-CoV-2 Detection

Analytical Chemistry, Год журнала: 2024, Номер 96(8), С. 3291 - 3299

Опубликована: Фев. 2, 2024

Lateral flow assay (LFA)-based rapid antigen tests are experiencing extensive global uptake as an expeditious and highly effective modality for the screening of viral infections during COVID-19 pandemic. While these devices have played a significant role in alleviating burden on public healthcare system, their specificity sensitivity fall short compared with molecular tests. In this study, we endeavor to address both limitations through utilization DNA nanotechnology LFA format, wherein substitute target-specific antibody designer nanostructure-based probes recognizing SARS-CoV-2 virus via multivalent, pattern-matching interactions. We meticulously designed Net-shaped nanostructure strategically arranged trimeric clusters aptamers that specifically recognize spike proteins SARS-CoV-2. This approach has proven instrumental bolstering virus-binding affinity LFAs. Our findings indicate high sensitivity, enabling detection loads ranging from 103 108 copies/mL. notable is maintained across various strains, obviating need intricate sample preparation protocols. The significance heightened lies crucial by nanostructure, which facilitates extremely low levels loads. not only enhances overall reliability self-testing but also reduces likelihood false-negative results, especially cases load within patient samples.

Язык: Английский

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Lateral nanoarchitectonics from nano to life: Ongoing challenge in interfacial chemical science

Chemical Science, Год журнала: 2024, Номер 15(45), С. 18715 - 18750

Опубликована: Янв. 1, 2024

The construction of functional structures within a single plane still has lot challenges. This review article will help us find new groundbreaking directions in lateral nanoarchitectonics toward advanced material organization.

Язык: Английский

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Sensitive amperometric immunosensor for pathogen antigen based on MoS2@AuNPs assembling dual-peptide as bioprobes with significant dual signal amplification

Analytica Chimica Acta, Год журнала: 2025, Номер 1355, С. 344015 - 344015

Опубликована: Апрель 6, 2025

Язык: Английский

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Dual Signal Amplification by Urease Catalysis and Silver Nanoparticles for Ultrasensitive Colorimetric Detection of Nucleic Acids

Analytical Chemistry, Год журнала: 2023, Номер 95(30), С. 11359 - 11364

Опубликована: Июль 19, 2023

Signal amplification techniques are highly desirable for the analysis of low-level targets that closely related with diseases and monitoring important biological processes. However, it is still challenging to achieve this goal in a facile economical way. Herein, we developed novel dual signal strategy by combining urease catalysis release Ag+ from silver nanoparticles (AgNPs). This was used quantifying DNA sequence (HIV-1) human immunodeficiency virus (HIV). target HIV-1 hybridizes capture probe on magnetic beads reporter AgNPs, forming sandwich complex. The captured AgNPs then transformed into numerous ions inactivate ureases. Without catalytic production ammonia urea, substrate solution shows low pH 5.8 will increase otherwise. change monitored indicator (phenol red), which allows colorimetric detection. proposed approach sensitive, easy use, economic, universal, exhibiting detection limit 9.7 fM (i.e., 1.94 attomoles) dynamic linear range 4 orders

Язык: Английский

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Sensitive dual-channel lateral flow immunoassay tagged with high brightness latex microsphere for simultaneous detection of respiratory viral antigens

Chemical Engineering Journal, Год журнала: 2024, Номер 497, С. 154826 - 154826

Опубликована: Авг. 13, 2024

Язык: Английский

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Comprehensive genomic analysis of the SARS-CoV-2 Omicron variant BA.2.76 in Jining City, China, 2022

BMC Genomics, Год журнала: 2024, Номер 25(1)

Опубликована: Апрель 17, 2024

Abstract Objective This study aims to analyze the molecular characteristics of novel coronavirus (SARS-CoV-2) Omicron variant BA.2.76 in Jining City, China. Methods Whole-genome sequencing was performed on 87 cases SARS-CoV-2 infection. Evolutionary trees were constructed using bioinformatics software sequence homology, sites, N-glycosylation and phosphorylation sites. Results All whole-genome sequences classified under evolutionary branch BA.2.76. Their similarity reference strain Wuhan-Hu-1 ranged from 99.72 99.74%. In comparison Wuhan-Hu-1, exhibited 77–84 nucleotide differences 27 deletions. A total 69 amino acid 9 deletions, 1 stop codon mutation identified across 18 proteins. Among them, spike (S) protein highest number ORF8 showed a Q27 mutation. Multiple proteins displayed variations glycosylation Conclusion continues evolve, giving rise new strains with enhanced transmission, stronger immune evasion capabilities, reduced pathogenicity. The application high-throughput technologies epidemic prevention control COVID-19 provides crucial insights into virus at genomic level, thereby holding significant implications for pandemic.

Язык: Английский

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