Опубликована: Янв. 1, 2024
Язык: Английский
Frontiers in Pharmacology, Год журнала: 2025, Номер 16
Опубликована: Апрель 7, 2025
Tiebangchui (TBC, Tibetan name: བང་ང་ནག་པ།), the dried tuberous root of Aconitum pendulum Busch. and flavaum Hand.-Mazz., is a prevalent used medicine, recognized for its significant therapeutic effects despite high toxicity. It commonly employed in treating diseases categorized as "Long" (རླུང་ནད།), cold, "Huang-shui" (སེར་ཆུ་ནད།), leprosy, mania medicine. Notably, it utilized treatment rheumatoid arthritis, which classified under disease category according to medical theory. Given considerable toxicity, various processing techniques aimed at reducing harmful TBC are essential safe application clinical settings. Hezi-decoction-processed method distinctive effective traditional but overall variability chemical constituents still unclear. This investigation sought examine variety diterpenoid alkaloids tanning constituents, identify potential metabolic markers differentiating unprocessed varying times, determine optimal time toxicity maintaining efficacy. A combination metabolomic was developed, integrating ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) with desorption electrospray ionisation imaging (DESI-MSI) coupled quantitative analytical techniques. done objective monitoring dynamic alterations during time. Metabolic were observed via DESI-MSI, three five tannin acids quantified through use UPLC HPLC. Fifty-one compounds identified processed samples, 31 discernible from TBC. total 22 markers, such aconine, aconitine, benzoylaconine, chebulic acid, gallic corilagin, can proficiently distinguish between raw different times. And results content determination tannins showed that they stabilized 72 h. The monoester-diterpenoid (MDAs) diester-diterpenoid (DDAs) levels 0.0149% 0.0852% h, respectively. contents 1,2,3,4,6-O-pentagalloylglucose, chebulinic ellagic acid 8.9706, 9.3444, 1.2438, 5.7582, 3.1160 mg/g, distribution accumulation investigated by DESI-MS. DESI-MSI consistent those experiments. Combined multivariate statistical analysis, h demonstrated be appropriate attenuation efficacy reservation implementation this technique could contribute identification Hezi decoction-processed establishment quality control evaluation procedures ensure safety proposed has elucidate mechanism medicines other toxic Chinese medicines, given wide applicability.
Язык: Английский
Процитировано
1
Journal of Mass Spectrometry, Год журнала: 2024, Номер 59(5)
Опубликована: Апрель 16, 2024
Abstract The use of mass spectrometry (MS) to acquire molecular images biological tissues and other substrates has developed into an indispensable analytical tool over the past 25 years. Imaging technologies are widely used today study in situ spatial distributions for a variety analytes. Early MS were acquired using secondary ion matrix‐assisted laser desorption/ionization. Researchers have also designed ionization techniques recent years probe surfaces generate images, including desorption electrospray (DESI), nanoDESI, ablation ionization, infrared ionization. Investigators now plethora select from when performing imaging experiments. This brief perspective will highlight utility relative figures merit these within context their spectrometry.
Язык: Английский
Процитировано
7
PROTEOMICS, Год журнала: 2025, Номер unknown
Опубликована: Янв. 10, 2025
ABSTRACT Alzheimer's disease (AD) is a leading cause of dementia, but the pathogenesis mechanism still elusive. Advances in proteomics have uncovered key molecular mechanisms underlying AD, revealing complex network dysregulated pathways, including amyloid metabolism, tau pathology, apolipoprotein E (APOE), protein degradation, neuroinflammation, RNA splicing, metabolic dysregulation, and cognitive resilience. This review examines recent proteomic findings from AD brain tissues biological fluids, highlighting potential biomarkers therapeutic targets. By examining landscape them, we aim to deepen our understanding support developing precision medicine strategies for more effective interventions.
Язык: Английский
Процитировано
1
Toxicologic Pathology, Год журнала: 2025, Номер unknown
Опубликована: Фев. 10, 2025
Recent advances in bioanalytical and imaging technologies have revolutionized our ability to assess complex biological pathological changes within tissue samples. Spatial omics, a rapidly evolving technology, enables the simultaneous detection of multiple biomolecules sections, allowing for high-dimensional molecular profiling microanatomical contexts. This offers powerful opportunity precise, multidimensional exploration disease pathophysiology. The Pathology 2.0 working group European Society Toxicologic (ESTP) includes subgroup dedicated spatial omics technologies. Their primary goal is raise awareness about these emerging their potential applications discovery toxicologic pathology. review provides an overview commonly used, commercially available platforms transcriptomic, proteomic, multiomic analysis, discussing technical aspects illustrative examples applications. To harness power translational drug human safety risk assessment, we emphasize important role pathologists at every stage workflow—from hypothesis generation sample preparation, data interpretation. offer novel opportunities target discovery, lead selection, preclinical clinical development compound development.
Язык: Английский
Процитировано
1
Green Chemistry, Год журнала: 2024, Номер 26(9), С. 4975 - 4986
Опубликована: Янв. 1, 2024
Vital aspects to consider when implementing green analytical chemistry in mass spectrometry.
Язык: Английский
Процитировано
6
Trends in Environmental Analytical Chemistry, Год журнала: 2024, Номер 42, С. e00228 - e00228
Опубликована: Фев. 3, 2024
Язык: Английский
Процитировано
4
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(14), С. 7752 - 7752
Опубликована: Июль 15, 2024
Breast cancer, a complex disease with significant prevalence to form metastases, necessitates novel therapeutic strategies improve treatment outcomes. Here, we present the results of comparative molecular study primary breast tumours, their and corresponding cell lines using Desorption Electrospray Ionisation (DESI) Laser-Assisted Rapid Evaporative Mass Spectrometry (LA-REIMS) imaging. Our show that ambient ionisation mass spectrometry technology is suitable for rapid characterisation samples, providing lipid- metabolite-rich spectrum within seconds. demonstrates lipidomic fingerprint tumour not significantly distinguishable from its metastasis, in parallel similarity observed between respective lines. While differences were tumours lines, distinct signatures several phospholipids such as PA(36:2), PE(36:1), PE(P-38:4)/PE(O-38:5) LA-REIMS imaging PE(P-38:4)/PE(O-38:5), PS(36:1), PI(38:4) DESI-MSI identified both cells. We tumours' characteristics can be found offering promising avenue assessing responsiveness interventions. A analysis by revealed complementary information, demonstrating utility cancer.
Язык: Английский
Процитировано
4
Mass Spectrometry, Год журнала: 2024, Номер 13(1), С. A0142 - A0142
Опубликована: Фев. 9, 2024
Among the most typical posttranslational modifications is glycosylation, which often involves covalent binding of an oligosaccharide (glycan) to either asparagine (N-linked) or a serine/threonine (O-linked) residue. Studies imply that N-glycan portion glycoprotein could serve as particular disease biomarker rather than protein itself because N-linked glycans have been widely recognized evolve with advancement tumors and other diseases. N-glycans found on sites especially significant. Since play clearly defined functions in folding proteins, cellular transport, transmission signals, them linked several illnesses. However, these N-glycans' production not template driven, they substantial morphological range, rendering it difficult distinguish species are relevant biology medicine using standard techniques. Mass spectrometry (MS) techniques emerged effective analytical tools for investigating role glycosylation health illness. This due developments MS equipment, data collection, sample handling By recording spatial dimension glycan's distribution
Язык: Английский
Процитировано
3
Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Янв. 2, 2025
Abstract Fast and reliable identification of bacteria directly in clinical samples is a critical factor microbiological diagnostics. Current approaches require time-consuming bacterial isolation enrichment procedures, delaying stratified treatment. Here, we describe biomarker-based strategy that utilises small molecular metabolites lipids for direct detection complex using mass spectrometry (MS). A spectral metabolic library 233 species mined markers showing specificity at different phylogenetic levels. Using univariate statistical analysis method, determine 359 so-called taxon-specific (TSMs). We apply these TSMs to the situ healthy cancerous gastrointestinal tissues as well faecal samples. To demonstrate MS method-agnostic nature, are analysed spatial metabolomics traditional bulk-based approaches. In this work, found >90% samples, suggesting general applicability workflow detect presence with standard MS-based analytical methods.
Язык: Английский
Процитировано
0
Elsevier eBooks, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0