Imaging with mass spectrometry: Which ionization technique is best?

Journal of Mass Spectrometry, Journal Year: 2024, Volume and Issue: 59(5)

Published: April 16, 2024

Abstract The use of mass spectrometry (MS) to acquire molecular images biological tissues and other substrates has developed into an indispensable analytical tool over the past 25 years. Imaging technologies are widely used today study in situ spatial distributions for a variety analytes. Early MS were acquired using secondary ion matrix‐assisted laser desorption/ionization. Researchers have also designed ionization techniques recent years probe surfaces generate images, including desorption electrospray (DESI), nanoDESI, ablation ionization, infrared ionization. Investigators now plethora select from when performing imaging experiments. This brief perspective will highlight utility relative figures merit these within context their spectrometry.

Language: Английский

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Proteomic Insight Into Alzheimer's Disease Pathogenesis Pathways

PROTEOMICS, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

ABSTRACT Alzheimer's disease (AD) is a leading cause of dementia, but the pathogenesis mechanism still elusive. Advances in proteomics have uncovered key molecular mechanisms underlying AD, revealing complex network dysregulated pathways, including amyloid metabolism, tau pathology, apolipoprotein E (APOE), protein degradation, neuroinflammation, RNA splicing, metabolic dysregulation, and cognitive resilience. This review examines recent proteomic findings from AD brain tissues biological fluids, highlighting potential biomarkers therapeutic targets. By examining landscape them, we aim to deepen our understanding support developing precision medicine strategies for more effective interventions.

Language: Английский

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Points to Consider From the ESTP Pathology 2.0 Working Group: Overview on Spatial Omics Technologies Supporting Drug Discovery and Development

Toxicologic Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Recent advances in bioanalytical and imaging technologies have revolutionized our ability to assess complex biological pathological changes within tissue samples. Spatial omics, a rapidly evolving technology, enables the simultaneous detection of multiple biomolecules sections, allowing for high-dimensional molecular profiling microanatomical contexts. This offers powerful opportunity precise, multidimensional exploration disease pathophysiology. The Pathology 2.0 working group European Society Toxicologic (ESTP) includes subgroup dedicated spatial omics technologies. Their primary goal is raise awareness about these emerging their potential applications discovery toxicologic pathology. review provides an overview commonly used, commercially available platforms transcriptomic, proteomic, multiomic analysis, discussing technical aspects illustrative examples applications. To harness power translational drug human safety risk assessment, we emphasize important role pathologists at every stage workflow—from hypothesis generation sample preparation, data interpretation. offer novel opportunities target discovery, lead selection, preclinical clinical development compound development.

Language: Английский

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Unveiling the processing mechanism of Hezi-decoction-processed Tiebangchui: a synthesis approach using UPLC-Q-TOF-MS-based metabolomics and DESI-MSI

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 7, 2025

Tiebangchui (TBC, Tibetan name: བང་ང་ནག་པ།), the dried tuberous root of Aconitum pendulum Busch. and flavaum Hand.-Mazz., is a prevalent used medicine, recognized for its significant therapeutic effects despite high toxicity. It commonly employed in treating diseases categorized as "Long" (རླུང་ནད།), cold, "Huang-shui" (སེར་ཆུ་ནད།), leprosy, mania medicine. Notably, it utilized treatment rheumatoid arthritis, which classified under disease category according to medical theory. Given considerable toxicity, various processing techniques aimed at reducing harmful TBC are essential safe application clinical settings. Hezi-decoction-processed method distinctive effective traditional but overall variability chemical constituents still unclear. This investigation sought examine variety diterpenoid alkaloids tanning constituents, identify potential metabolic markers differentiating unprocessed varying times, determine optimal time toxicity maintaining efficacy. A combination metabolomic was developed, integrating ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) with desorption electrospray ionisation imaging (DESI-MSI) coupled quantitative analytical techniques. done objective monitoring dynamic alterations during time. Metabolic were observed via DESI-MSI, three five tannin acids quantified through use UPLC HPLC. Fifty-one compounds identified processed samples, 31 discernible from TBC. total 22 markers, such aconine, aconitine, benzoylaconine, chebulic acid, gallic corilagin, can proficiently distinguish between raw different times. And results content determination tannins showed that they stabilized 72 h. The monoester-diterpenoid (MDAs) diester-diterpenoid (DDAs) levels 0.0149% 0.0852% h, respectively. contents 1,2,3,4,6-O-pentagalloylglucose, chebulinic ellagic acid 8.9706, 9.3444, 1.2438, 5.7582, 3.1160 mg/g, distribution accumulation investigated by DESI-MS. DESI-MSI consistent those experiments. Combined multivariate statistical analysis, h demonstrated be appropriate attenuation efficacy reservation implementation this technique could contribute identification Hezi decoction-processed establishment quality control evaluation procedures ensure safety proposed has elucidate mechanism medicines other toxic Chinese medicines, given wide applicability.

Language: Английский

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Mass spectrometry in the age of green analytical chemistry

Green Chemistry, Journal Year: 2024, Volume and Issue: 26(9), P. 4975 - 4986

Published: Jan. 1, 2024

Vital aspects to consider when implementing green analytical chemistry in mass spectrometry.

Language: Английский

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Mass spectrometry imaging in environmental monitoring: From a scarce existing past to a promising future

Trends in Environmental Analytical Chemistry, Journal Year: 2024, Volume and Issue: 42, P. e00228 - e00228

Published: Feb. 3, 2024

Language: Английский

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Characterisation of Canine and Feline Breast Tumours, Their Metastases, and Corresponding Primary Cell Lines Using LA-REIMS and DESI-MS Imaging

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7752 - 7752

Published: July 15, 2024

Breast cancer, a complex disease with significant prevalence to form metastases, necessitates novel therapeutic strategies improve treatment outcomes. Here, we present the results of comparative molecular study primary breast tumours, their and corresponding cell lines using Desorption Electrospray Ionisation (DESI) Laser-Assisted Rapid Evaporative Mass Spectrometry (LA-REIMS) imaging. Our show that ambient ionisation mass spectrometry technology is suitable for rapid characterisation samples, providing lipid- metabolite-rich spectrum within seconds. demonstrates lipidomic fingerprint tumour not significantly distinguishable from its metastasis, in parallel similarity observed between respective lines. While differences were tumours lines, distinct signatures several phospholipids such as PA(36:2), PE(36:1), PE(P-38:4)/PE(O-38:5) LA-REIMS imaging PE(P-38:4)/PE(O-38:5), PS(36:1), PI(38:4) DESI-MSI identified both cells. We tumours' characteristics can be found offering promising avenue assessing responsiveness interventions. A analysis by revealed complementary information, demonstrating utility cancer.

Language: Английский

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Universal, untargeted detection of bacteria in tissues using metabolomics workflows

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 2, 2025

Abstract Fast and reliable identification of bacteria directly in clinical samples is a critical factor microbiological diagnostics. Current approaches require time-consuming bacterial isolation enrichment procedures, delaying stratified treatment. Here, we describe biomarker-based strategy that utilises small molecular metabolites lipids for direct detection complex using mass spectrometry (MS). A spectral metabolic library 233 species mined markers showing specificity at different phylogenetic levels. Using univariate statistical analysis method, determine 359 so-called taxon-specific (TSMs). We apply these TSMs to the situ healthy cancerous gastrointestinal tissues as well faecal samples. To demonstrate MS method-agnostic nature, are analysed spatial metabolomics traditional bulk-based approaches. In this work, found >90% samples, suggesting general applicability workflow detect presence with standard MS-based analytical methods.

Language: Английский

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Strategies for the direct coupling of sampling and sample preparation to ambient mass spectrometry

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Psychobiotics and the gut-brain axis: advances in metabolite quantification and their implications for mental health

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 20

Published: Feb. 5, 2025

Psychobiotics are live microorganisms that, when administered in adequate amounts, confer mental health benefits to the host. Several clinical studies have demonstrated significant from psychobiotic administration, making them an emerging topic food science. Certain strains of Lactobacillus, Bifidobacterium, Streptococcus, Escherichia, and Enterococcus species known for their ability modulate gut-brain axis provide benefits. Proposed action mechanisms include production neuroactive compounds or precursors, which may cross blood-brain barrier, transported by extracellular vesicles. However, there is a lack vivo evidence directly confirming these mechanisms, although indirect recent suggest potential pathways further investigation. To advance our understanding, it crucial study within host, with accurate quantification and/or precursors being key such studies. Current methods, however, face challenges, as low sensitivity detecting trace metabolites limited specificity due interference other compounds, impacting reliability measurements. This review discusses field psychobiotics, compound estimation techniques, perspectives improvement quantifying

Language: Английский

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