Truncated PD1 Engineered Gas‐Producing Extracellular Vesicles for Ultrasound Imaging and Subsequent Degradation of PDL1 in Tumor Cells
Advanced Science,
Год журнала:
2024,
Номер
11(12)
Опубликована: Янв. 23, 2024
PDL1
blockade
therapy
holds
great
promise
in
cancer
immunotherapy.
Ultrasound
imaging
of
expression
the
tumor
is
importance
predicting
therapeutic
efficacy.
As
a
proof-of-concept
study,
novel
ultrasound
contrast
agent
has
been
innovated
here
to
image
and
block
tissue.
Briefly,
extracellular
vesicles
(EVs)
are
engineered
display
truncated
PD1
(tPD1)
on
surface
bind
with
high
affinity
by
fusion
EV-abundant
transmembrane
protein
PTGFRN.
The
EVs
then
encapsulated
Ca(HCO
Язык: Английский
Engineered extracellular vesicle-based gene therapy for the treatment of discogenic back pain
Biomaterials,
Год журнала:
2024,
Номер
308, С. 122562 - 122562
Опубликована: Апрель 1, 2024
Painful
musculoskeletal
disorders
such
as
intervertebral
disc
(IVD)
degeneration
associated
with
chronic
low
back
pain
(termed
"Discogenic
pain",
DBP),
are
a
significant
socio-economic
burden
worldwide
and
contribute
to
the
growing
opioid
crisis.
Yet
there
very
few
if
any
successful
interventions
that
can
restore
tissue's
structure
function
while
also
addressing
symptomatic
pain.
Here
we
have
developed
novel
non-viral
gene
therapy,
using
engineered
extracellular
vesicles
(eEVs)
deliver
developmental
transcription
factor
FOXF1
degenerated
IVD
in
an
vivo
model.
Injured
IVDs
treated
eEVs
loaded
demonstrated
robust
sex-specific
reductions
behaviors
compared
control
groups.
Furthermore,
restoration
of
animals
were
observed,
increases
height,
tissue
hydration,
proteoglycan
content,
mechanical
properties.
This
is
first
study
successfully
modulating
animal
model
DBP
eEV-based
delivery
genes.
Such
strategy
be
readily
translated
other
painful
disorders.
Язык: Английский
Multiplex Detection of Biomarkers Empowered by Nanomaterials
Precision Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 21, 2025
Язык: Английский
Review of recent preclinical and clinical research on ligand-targeted liposomes as delivery systems in triple negative breast cancer therapy
Journal of Liposome Research,
Год журнала:
2024,
Номер
34(4), С. 671 - 696
Опубликована: Март 23, 2024
Triple-negative
breast
Cancer
(TNBC)
is
one
of
the
deadliest
types,
making
up
about
20%
all
cancers.
Chemotherapy
traditional
manner
progressed
TNBC
treatment;
however,
it
has
a
short-term
result
with
high
reversibility
pace.
The
lack
targeted
treatment
limited
and
person-dependent
options
for
those
suffering
from
cautions
to
be
worst
type
cancer
among
patients.
Consequently,
appropriate
this
disease
considered
major
clinical
challenge.
Therefore,
various
methods
have
been
developed
treat
TNBC,
which
chemotherapy
most
common
well-known
approach
recently
studied.
Although
effective
are
chemotherapies,
they
often
accompanied
by
critical
limitations,
especially
specific
functionality.
These
lead
systematic
toxicity
and,
ultimately,
expansion
multidrug-resistant
(MDR)
cells.
finding
novel
efficient
techniques
enhance
targeting
an
essential
requirement.
Liposomes
demonstrated
that
method
drug
delivery;
large
number
liposome-based
delivery
systems
annually
developed,
small
just
received
authorization
application.
new
approaches
using
liposomes
target
their
structure
ligands
increase
therapeutic
efficiency
diminish
undesired
side
effects
on
body
tissues.
current
study
describes
recent
strategies
research
associated
functionalizing
liposomes'
different
as
carriers
in
treating
TNBCs
preclinical
stages.
Язык: Английский
Tissue Nanotransfection Silicon Chip and Related Electroporation-Based Technologies for In Vivo Tissue Reprogramming
Nanomaterials,
Год журнала:
2024,
Номер
14(2), С. 217 - 217
Опубликована: Янв. 19, 2024
Tissue
nanotransfection
(TNT),
a
cutting-edge
technique
of
in
vivo
gene
therapy,
has
gained
substantial
attention
various
applications
ranging
from
tissue
reprogramming
regenerative
medicine,
and
wound
healing
to
cancer
treatment.
This
harnesses
the
advancements
semiconductor
processes,
facilitating
integration
conventional
transdermal
delivery
methods—nanoelectroporation
microneedle
technologies.
TNT
silicon
chips
have
demonstrated
considerable
promise
fibroblast
cells
skin
into
vascular
or
neural
preclinical
studies
assist
recovery
injured
limbs
damaged
brain
tissue.
More
recently,
application
been
extended
area
exosomes,
which
are
vital
for
intracellular
communication
track
their
functionality
during
process.
In
this
review,
we
provide
an
in-depth
examination
design,
fabrication,
chips,
alongside
critical
analysis
electroporation-based
transfer
mechanisms.
Additionally,
review
discussed
existing
limitations
challenges
current
technique,
may
project
future
trajectories
landscape
therapy.
Through
exploration,
aims
shed
light
on
prospects
broader
context
therapy
regeneration.
Язык: Английский
Exosomes and breast cancer angiogenesis; Highlights in intercellular communication
Cancer Cell International,
Год журнала:
2024,
Номер
24(1)
Опубликована: Дек. 18, 2024
Breast
cancer
(BC)
is
a
prevalent
and
highly
lethal
in
females.
Like
other
types,
the
intricate
cellular
molecular
heterogeneity
leads
to
variation
of
therapeutic
outcomes.
The
development
progression
blood
vessels
increase
tumor
cell
expansion
metastasis
remote
sites.
Based
on
several
pieces
scientific
data,
different
mediators
cells
are
involved
promotion
angiogenesis
into
parenchyma.
Recent
data
have
indicated
critical
role
extracellular
vesicles,
especially
exosomes
(Exos),
transfer
molecules
between
BC
cells.
Due
unique
physicochemical
properties,
certain
signaling
molecules,
Exos
at
center
attention
terms
biomarkers
bullets
patients.
Along
with
these
statements,
understanding
modulatory
seems
clinical
setting.
Here,
mechanisms
by
which
can
orchestrate
phenomenon
via
discussed
detail.
present
study
help
us
understand
pro-/anti-angiogenesis
design
better
oncostatic
strategies.
Язык: Английский
Tissue nano-transfection of antimicrobial genes drives bacterial biofilm killing in wounds and is potentially mediated by extracellular vesicles
Journal of Controlled Release,
Год журнала:
2024,
Номер
376, С. 1300 - 1315
Опубликована: Ноя. 13, 2024
Язык: Английский
Quality control and validation of extracellular vesicles isolated from cultured human breast cancer cells
BMC Research Notes,
Год журнала:
2024,
Номер
17(1)
Опубликована: Июль 23, 2024
Abstract
Objective
Extracellular
vesicles
(EVs)
have
been
shown
to
play
a
critical
role
in
promoting
tumorigenesis.
As
EV
research
grows,
it
is
of
importance
standardization
isolation,
quality
control,
characterization
and
validation
methods
across
studies
along
with
reliable
references
explore
troubleshooting
solutions.
Therefore,
our
objective
this
Research
Note
was
isolate
EVs
from
multiple
breast
cancer
cell
lines
describe
perform
protocols
for
as
outlined
by
the
list
minimal
information
(MISEV)
International
Society
Vesicles.
Results
To
EVs,
two
techniques
were
employed:
ultracentrifugation
size
exclusion
chromatography.
Ultracentrifugation
yielded
better
recovery
hands
therefore
used
further
validation.
In
order
satisfy
MISEV
requirements,
protein
quantification,
immunoblotting
positive
(CD9,
CD63,
TSG101)
negative
(TGFβ1,
β-tubulin)
markers,
nanoflow
cytometry
electron
microscopy
performed.
With
these
experiments,
we
demonstrate
that
yield
validated
varied
between
different
lines.
Protocols
optimized
accommodate
low
levels
various
technical
suggestions
are
included
potential
application
other
types
may
provide
benefit
investigators
interested
future
studies.
Язык: Английский