Hydrogel forming microneedles loaded with VEGF and Ritlecitinib/polyhydroxyalkanoates nanoparticles for mini-invasive androgenetic alopecia treatment

Bioactive Materials, Год журнала: 2024, Номер 38, С. 95 - 108

Опубликована: Апрель 23, 2024

Androgenetic alopecia (AGA), the most prevalent clinical hair loss, lacks safe and effective treatments due to downregulated angiogenic genes insufficient vascularization in perifollicular microenvironment of bald scalp AGA patients. In this study, a hyaluronic acid (HA) based hydrogel-formed microneedle (MN) was designed, referred as V-R-MNs, which simultaneously loaded with vascular endothelial growth factor (VEGF) novel loss drug Ritlecitinib, latter is encapsulated slowly biodegradable polyhydroxyalkanoates (PHAs) nanoparticles (R-PHA NPs) for minimally invasive treatment. The integration HA hydrogel alongside PHA significantly bolstered mechanical characteristics microneedles enhanced skin penetration efficiency. Due biosafety, strength, controlled degradation properties formed microneedles, V-R-MNs can effectively penetrate skin's stratum corneum, facilitating direct delivery VEGF Ritlecitinib invasive, painless long-term sustained release manner. not only promoted angiogenesis improve immune around follicle promote proliferation development cells, but also application MNs produce certain stimulation could angiogenesis. comparison minoxidil treatment, regeneration effect V-R-MN model mice characterized by rapid onset anagen phase, improved quality, greater coverage. This introduces new, clinically safer, more efficient strategy serving reference treatment other related diseases.

Язык: Английский

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3D bioprinting advanced biomaterials for craniofacial and dental tissue engineering – A review

Materials & Design, Год журнала: 2024, Номер 241, С. 112886 - 112886

Опубликована: Март 28, 2024

The rising incidence of defects in oral and maxillofacial tissues, linked to factors such as trauma, tumors, periodontal disease, aging, poses significant challenges. Current treatments, involving autografts, allografts, synthetic graft materials, face obstacles like secondary inflammation, inadequate biocompatibility. Tissue engineering, integrating cell biology material science since the 1990s, relies heavily on biomaterial scaffolds promote adhesion, proliferation, differentiation. Traditional scaffold fabrication, including 3D printing, methods lack precision, hindering effective tissue repair by controlling distribution extracellular matrix. Biomedical engineering advancements have introduced bioprinting an innovative solution, overcoming constraints conventional scaffolds. technology enables rapid precise reconstruction damaged tissues with loaded cells, mimicking vivo environments. This paper explores key technologies inkjet-based, extrusion-based, fused deposition modeling, laser-assisted, VAT photopolymerization, freeform reversible embedding suspended hydrogels, sacrificial template printing. selection materials suitable mechanical biological properties is crucial, considering distinct requirements each technique. review provides a comprehensive survey research progress printing applications craniofacial dental serving valuable reference for future medical research.

Язык: Английский

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Directed osteogenic differentiation of human bone marrow mesenchymal stem cells via sustained release of BMP4 from PBVHx-based nanoparticles

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 265, С. 130649 - 130649

Опубликована: Март 5, 2024

Язык: Английский

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Carbon Cycle of Polyhydroxyalkanoates (CCP): Biosynthesis and Biodegradation

Environmental Research, Год журнала: 2025, Номер unknown, С. 120904 - 120904

Опубликована: Янв. 1, 2025

Язык: Английский

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Current status and challenges in the application of microbial PHA particles

Particuology, Год журнала: 2023, Номер 87, С. 286 - 302

Опубликована: Сен. 9, 2023

Язык: Английский

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Recent advances of medical polyhydroxyalkanoates in musculoskeletal system.

PubMed, Год журнала: 2023, Номер 4(4), С. 234 - 247

Опубликована: Янв. 1, 2023

Infection and rejection in musculoskeletal trauma often pose challenges for natural healing, prompting the exploration of biomimetic organ tissue transplantation as a common alternative solution. Polyhydroxyalkanoates (PHAs) are large family biopolyesters synthesised microorganism, demonstrating excellent biocompatibility controllable biodegradability remodelling drug delivery. With different monomer-combination polymer-types, multi-mechanical properties PHAs making them have great application prospects medical devices with stretching, compression, twist long time, especially engineering. This review systematically summarises applications multiple tissues repair release, encompassing areas such bone, cartilage, joint, skin, tendons, ligament, cardiovascular tissue, nervous tissue. It also discusses encountered their application, including high production costs, potential cytotoxicity, uncontrollable particle size distribution. In conclusion, offer compelling avenue system applications, striking balance between mechanical performance. However, addressing requires further research to unleash full tackling complexities regeneration.

Язык: Английский

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Exploring the accumulation of Poly-β-hydroxybutyrate in the marine cyanobacterium Nostoc sp. BDU 00591: A comprehensive study on extraction, stimulation and characterisation

Biocatalysis and Agricultural Biotechnology, Год журнала: 2025, Номер unknown, С. 103488 - 103488

Опубликована: Янв. 1, 2025

Язык: Английский

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Integrated bioprocess development for the biosynthesis and characterization of polyhydroxybutyrate extracted from Streptomyces nigra KDS4

Sustainable Chemistry and Pharmacy, Год журнала: 2025, Номер 43, С. 101903 - 101903

Опубликована: Янв. 13, 2025

Язык: Английский

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Synthetic biology for medical biomaterials

Deleted Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 29, 2025

Abstract After more than 20 years of development, synthetic biology has emerged as an interdisciplinary field that integrates biology, medicine, mathematics, and engineering. By constructing regulating genetic elements, networks, pathways, artificially engineered bacteria, cells, or viruses can directly interact with the human body to enable disease treatment via biology. Additionally, platforms have been employed in production medical biomaterials (MBMs), indirectly contributing maintenance health. In this review, we present a range typical MBMs derived from platforms, including polylactic acid, polyhydroxyalkanoates, hyaluronic collagen, poly(β‐hydroxybutyrate), poly(β‐malic acid), poly‐γ‐glutamic alginate, chitosan, bacterial cellulose, antimicrobial peptides. We also introduce key techniques tools involved, such chassis cell design, gene expression regulation editing represented by CRISPRi, metabolic engineering, morphology cell‐free systems. Furthermore, summarize recent advancements strategies enhancing cost‐reduction, biosynthesis novel materials, material characteristics diversity, minimizing toxicity biosynthetic systems, designing living materials research applications clinical translation for MBMs. Finally, discuss emerging trends may shape future biomedical

Язык: Английский

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PHBV cycle of life using waste as a starting point: from production to recyclability

Frontiers in Materials, Год журнала: 2024, Номер 11

Опубликована: Июнь 3, 2024

Global concern about plastic pollution is forcing new policies and modifications of human consumption as well promoting research lines aiming at the replacement non-degradable plastics with other polymers more environmentally friendly. Addressing food waste circular economy strategies, among approaches, are crucial in reducing environmental impacts fostering sustainability several sectors like agri-food industry. The European Union’s Circular Economy Action Plan a significant initiative this direction. Biotechnological processes, especially valorisation to produce highly marketed biomolecules poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) using microorganisms cellular factories, offer promising avenues for achieving these goals. PHBV biodegradable polymer firstly characterised an isolated biopolymer from bacterial biomass. This shows interesting physicochemical properties making possible immense potential various applications due its biocompatibility sustainability, thus revealing it good candidate replace produced by chemical synthesis petroleum (which recalcitrant consequently pollutants). review critically analyses end-of-life scenarios their biological pathways, through forms biotechnological operation production, described until moment recycling.

Язык: Английский

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