CRISPR/Cas12a-enhanced DNA nanomachine for multiple respiratory pathogens detection DOI
Siyi Wang,

Yueli Hu,

Ziqiang Deng

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(99), P. 14814 - 14817

Published: Jan. 1, 2024

We constructed an ICP-MS analysis strategy of a CRISPR/Cas12a-enhanced DNA nanomachine to detect multiple respiratory pathogens.

Language: Английский

Comprehensive insights into pathogenicity and emerging CRISPR-integrated biosensors for the early detection of breast cancer biomarkers DOI

Ashok Saini,

Neeraj Dilbaghi, Neelam Yadav

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112908 - 112908

Published: Jan. 1, 2025

Language: Английский

Citations

1
Biomaterial-Based CRISPR/Cas9 Delivery Systems for Tumor Treatment DOI Creative Commons
Mengmeng Li, Fenglei Chen, Qian Yang

et al.

Biomaterials Research, Journal Year: 2024, Volume and Issue: 28

Published: Jan. 1, 2024

CRISPR/Cas9 gene editing technology is characterized by high specificity and efficiency, has been applied to the treatment of human diseases, especially tumors involving multiple genetic modifications. However, clinical application still faces some major challenges, most urgent which development optimized delivery vectors. Biomaterials are currently best choice for use in vectors owing their tunability, biocompatibility, efficiency. As research on biomaterial continues progress, hope system oncology therapy builds. In this review, we first detail its potential applications tumor therapy. Then, introduce different forms compare physical, viral, non-viral addition, analyze characteristics types We further review recent progress biomaterials as treat specific tumors. Finally, summarize shortcomings prospects biomaterial-based systems.

Language: Английский

Citations

4
Dual-Accelerated Signal Amplification in Biosensing via Spatial Confining Catalytic Hairpin Assembly-Activated Spherical CRISPR/Cas12a System for Trans-Cleavage of Hairpin DNA Reporters DOI

Zhuqi Sui,

Baoqiang Chen,

Jia Zhao

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression and implicated in various diseases, including cancer. Due to their critical role diagnostics, there is a growing need for sensitive, specific, rapid detection methods miRNAs. In this study, we present dual-accelerated signal amplification platform miRNA biosensing, which integrates spatial confining catalytic hairpin assembly (SC-CHA) with spherical CRISPR/Cas12a (S-CRISPR/Cas12a) system (SC-CHA@S-CRISPR/Cas12a) trans-cleavage of DNA reporters. The method employs biotinylated palindrome-rich sequence (PAS) form nanoballs, serve as scaffold the operation SC-CHA upon binding. products bind crRNA Cas 12a protein, activating S-CRISPR/Cas12a cleave reporter generate detectable fluorescence signal. uniqueness lies combined use nanoballs reporters, both significantly accelerate reaction kinetics, resulting generation. Additionally, nanostructure, integrated system, greatly enhances biostability accelerating kinetics. These features enable exhibit high sensitivity, limit (LOD) low 13.75 fM, excellent specificity, successfully distinguishing miRNA-21 from other assay also biostable, demonstrating reliable performance complex biological samples such human serum. This dual-acceleration approach offers promising solution rapid, specific potential applications early cancer diagnosis clinical monitoring.

Language: Английский

Citations

0
Development of a Rapid On-Site Detection Method for Largemouth Bass Virus Based on Rpa-Crispr/Cas12a System DOI
Na Li, Chunmei Dai, Yuan Mao

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Harnessing the power of clustered regularly interspaced short palindromic repeats (CRISPR) based microfluidics for next-generation molecular diagnostics DOI
Rasanpreet Kaur, Saurabh Kumar Gupta, Arjun Chauhan

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: Aug. 8, 2024

Language: Английский

Citations

3
Establishment of a Lateral Flow Dipstick Detection Method for Influenza A Virus Based on CRISPR/Cas12a System DOI
Xiaoyan Zhao,

Ximing Zheng,

Xiyong Yang

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(37), P. 946 - 952

Published: Jan. 1, 2024

This study aimed to develop a rapid, visual PCR-CRISPR/Cas12-LFD method for detecting influenza A by utilizing the conserved region of matrix protein gene.

Language: Английский

Citations

2
Recent progress in prompt molecular detection of liquid biopsy using Cas enzymes: innovative approaches for cancer diagnosis and analysis DOI Creative Commons

Zahra Farshchian Yazdi,

Shahrzad Roshannezhad,

Samaneh Sharif

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 31, 2024

Creating fast, non-invasive, precise, and specific diagnostic tests is crucial for enhancing cancer treatment outcomes. Among methods, those relying on nucleic acid detection are highly sensitive specific. Recent developments in technologies, particularly leveraging Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), revolutionizing detection, providing accurate timely results. In clinical oncology, liquid biopsy has become a noninvasive early-detectable alternative to traditional biopsies over the last two decades. Analyzing content of samples, which include Circulating Tumor Cells (CTCs), DNA (ctDNA), Cell-Free RNA (cfRNA), tumor extracellular vesicles, provides method monitoring. this review, we explore how characteristics various Cas (CRISPR-associated) enzymes have been utilized assays highlight their main applications innovative approaches monitoring, as well early rapid cancers.

Language: Английский

Citations

2
Liquid biopsy applications in oral oncology: Clinical utility and future directions DOI Creative Commons
Vinay Sundaram,

M. Sivasubramani

Oral Oncology Reports, Journal Year: 2024, Volume and Issue: 10, P. 100449 - 100449

Published: April 26, 2024

Language: Английский

Citations

0
CRISPR/Cas12a-enhanced DNA nanomachine for multiple respiratory pathogens detection DOI
Siyi Wang,

Yueli Hu,

Ziqiang Deng

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(99), P. 14814 - 14817

Published: Jan. 1, 2024

We constructed an ICP-MS analysis strategy of a CRISPR/Cas12a-enhanced DNA nanomachine to detect multiple respiratory pathogens.

Language: Английский

Citations

0