Recent Developments in Stimuli-Responsive Polymer for Emerging Applications: A Review

Results in Engineering, Год журнала: 2025, Номер unknown, С. 103900 - 103900

Опубликована: Янв. 1, 2025

Язык: Английский

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Cytochrome P450 electrochemical biosensors transforming in vitro metabolism testing – Opportunities and challenges

Bioelectrochemistry, Год журнала: 2025, Номер 163, С. 108913 - 108913

Опубликована: Янв. 22, 2025

The ability of the living world to flourish in face constant exposure dangerous chemicals depends on management a widespread group enzymes known as heme-thiolate monooxygenases or cytochrome P450 superfamily. About three-quarters all reactions determining metabolism endogenous compounds, those carried foods, taken drugs, even synthetic discarded into environment depend their catalytic performance. chromatographic and (photo)luminometric methods routinely used predictive analytical tools laboratories have significant drawbacks ranging from limited shelf-life reagents, use substrates, laborious tedious procedures for highly sensitive detection. In this review, alternative electrochemical biosensors using bio-element are emphasized main aspects well regarding implementation usefulness. Despite various schemes proposed implementation, reports real applications scant several reasons, including low reaction rates, broad substrate specificity, uncoupling occurrence, need expensive electron transfer partners promote transfer. Finally, prospect future developments is introduced, focusing integrating miniaturized systems with techniques, alongside optimizing enzyme immobilization electrode modifications improve enzymatic stability enhance sensor reliability. This progress represents crucial step towards creation portable that mimic human physiological responses, supporting precision medicine approach.

Язык: Английский

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Molecular sieve redefines SPR sensible range for “win-win” dual functions to enhance the sensitization and anti-fouling

Nano Today, Год журнала: 2025, Номер 61, С. 102670 - 102670

Опубликована: Фев. 15, 2025

Язык: Английский

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Novel High‐Throughput Electrochemical Detection of Staphylococcus Aureus, Bacillus Cereus, or Micrococcus Luteus Using AuNPs@Ti₃C₂T_z Functionalized with Sandwich Peptides

Small, Год журнала: 2025, Номер unknown

Опубликована: Март 18, 2025

Abstract Affinity‐based electrochemical biosensors hold promise for detecting pathogenic bacteria in environmental applications. This study focuses on gram‐positive bacteria, which can cause fatal infections and are a major global mortality factor. An biosensor platform using high‐throughput 16‐channel gold disk electrodes (16‐GDEs) inspired by bio‐microelectromechanical systems (BioMEMS) is developed, it incorporates nanocomposite (AuNPs@Ti 3 C 2 T z ) with sandwich peptide structures to enhance electroconductivity biological antifouling. Using AuNPs@Ti ‐coated 16‐GDEs, sensitive ( Staphylococcus aureus , Bacillus cereus or Micrococcus luteus constructed validated fresh‐water samples spiking showed linear correlations between normalized peak current logarithmic concentrations of the target (adjusted R‐square ≥ 0.93). A single containing S. M. B. also exhibiting specific responses without any cross‐reactivity real samples. enabled simultaneous detection multiple analytes (500 CFU mL⁻¹) be applied detect analyte suitable pair. The strategy implement quantitative real‐time polymerase chain reaction (RT‐qPCR) adaptive sensing device successfully bacteria. nanocomposite‐enabled 16‐GDEs offers valuable tool clinical diagnostics.

Язык: Английский

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Functionalized screen-printed electrodes for non-invasive detection of vascular-endothelial cadherin in extracellular vesicles

RSC Advances, Год журнала: 2025, Номер 15(16), С. 12609 - 12621

Опубликована: Янв. 1, 2025

Detection of endothelial biomarker vascular-endothelial cadherin (CD144) on saliva-derived extracellular vesicles using a gold screen-printed electrode biosensor functionalized with mercaptoundecanoic acid and anti-CD144 antibody.

Язык: Английский

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Interface Regulated Solid‐State Rectification of Peptide via Quantum Dots Self‐Assembly Monolayer

Small, Год журнала: 2025, Номер unknown

Опубликована: Май 10, 2025

Abstract Peptides have been demonstrated as promising candidates for constructing bioelectronic devices. The charge transport through peptides can be effectively modulated via intrinsic composition, structure, and, most notably, interfacial engineering. However, achieving specific electrical properties, such solid‐state rectification, remains a challenge in peptide‐based bioelectronics. To fill this gap, heterojunction is designed based on the uniform and densely‐packed bilayers composed of peptide quantum dots (QDs) self‐assembled monolayer. presence QDs monolayer markedly regulates rectification static dielectric constant ( ɛ r ) heterojunctions. ratio junctions raised up to ≈10 3 , increases by factor 3. Both peptide/QDs heterojunctions increase with enhanced non‐covalent interactions between side chains QDs. interaction modulates coupling across interface, influencing energy level alignment at interface. This leads distinct pathways under opposite bias polarities, thus regulation rectification. These findings suggest that strengthening both . deepens understanding interface‐regulated molecular offers theoretical framework optimizing biomolecular

Язык: Английский

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Probing the Structure and Orientation of Carboxylic Acid-Terminated Self-Assembled Monolayers

Langmuir, Год журнала: 2024, Номер 40(36), С. 18925 - 18941

Опубликована: Авг. 27, 2024

Self-assembled monolayers (SAMs), such as alkanethiols (AT), are widely used functional coatings or interfaces between different materials. There is an assumption that the arrangement and alignment of hydrocarbon chains in films made from carboxyl-terminated similar to those alkanethiols. Here, structure outermost layer near-surface region SAMs formed various lengths has been analyzed. The chemical composition samples was measured using X-ray photoelectron spectroscopy (XPS) angle-resolved XPS (AR-XPS), allowing film thickness. Metastable induced (MIES) a surface analytical tool sensitive only for conjunction with density theory (DFT) calculations provided insights into topmost layer, showing it consists mainly backbone SAM-forming molecules. Through combining AR-XPS concentration depth profiles measurement can be shown tend favor gauche orientation, enabling interactions groups.

Язык: Английский

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Self-assembly Monolayer Fluorescent Probe on Alumina Substrate Enabling Highly Sensitive Detection of Arsenite Ions in Groundwater

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 424, С. 136882 - 136882

Опубликована: Ноя. 8, 2024

Язык: Английский

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Modelling Prospects of Bio-Electrochemical Immunosensing Platforms

Electrochem, Год журнала: 2024, Номер 5(2), С. 146 - 161

Опубликована: Апрель 24, 2024

Electrochemistry is a hotspot in today’s research arena. Many different domains have been extended for their role towards the Internet of Things, digital health, personalized nutrition, and/or wellness using electrochemistry. These advances led to substantial increase power and popularity electroanalysis its expansion into new phases environments. The recent COVID-19 pandemic, which turned our lives upside down, has helped us understand need miniaturized electrochemical diagnostic platforms. It also accelerated mobile wearable, implantable sensors as telehealth systems. major principle behind these platforms immunoassays, help overshadowing classical gold standard methods (reverse transcriptase polymerase chain reaction) terms accuracy, time, manpower, and, most importantly, economics. groups endeavoured use bio-electrochemical tools overcome limitations assays (in accessibility, portability, response time). This review mainly focuses on technologies used immunosensing platforms, fabrication requirements, mechanistic objectives, techniques involved, subsequent output signal amplifications tagged non-tagged system. combination various (optical spectroscopy, Raman scattering, column chromatography, HPLC, X-ray diffraction) enabled construction high-performance electrodes. Later review, combinations utilization will be explained platform along with chemical bonding later part article. Furthermore, market study real prototypes elaborately discussed.

Язык: Английский

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Incorporation of phenylcarbonyl groups in the sidechain: A tool to induce ordered assembly of peptides on surfaces

Journal of Peptide Science, Год журнала: 2024, Номер unknown

Опубликована: Июнь 19, 2024

The possibility of introducing various functionalities on peptides with relative ease allows them to be used for molecular applications. However, oligopeptides prepared entirely from proteinogenic amino acids seldom assemble as ordered structures surfaces. Therefore, sidechain modifications that can increase the intermolecular interactions without altering constitution a given peptide become an attractive route self-assembling We find replacing phenylalanine residues unusual have phenylcarbonyl sidechains in increases formation self-assembly highly pyrolytic graphite surface. Peptides containing modified provided extended long-range assemblies, while analogous failed form assemblies. X-ray crystallographic analysis bulk these and reveal such do not alter secondary structure crystals. It also reveals hydrogen bonding interaction through facilitates growth graphite.

Язык: Английский

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