An Advanced Combinatorial System from Vitis vinifera Leaves and Propolis Enhances Antioxidants’ Skin Delivery and Fibroblasts Functionality DOI Creative Commons
Sophia Athanasopoulou, Eleni Spanidi, Eleni Panagiotidou

и другие.

Pharmaceuticals, Год журнала: 2024, Номер 17(12), С. 1610 - 1610

Опубликована: Ноя. 29, 2024

Background/Objectives: Vine leaves are a bulky by-product that disposed of and treated as waste in the wine production process. In present study polyphenols from vine were extracted simultaneously encapsulated new delivery system consisting liposomes cyclodextrins. This was further combined with propolis cyclodextrins, resulting colloidal suspension for release antioxidants time-controlled way, rate which depends on ratio materials. The result is raw material exhibits antioxidant ECM protective effects when administered skin fibroblasts (NHDFs). Methods: promoting efficacy produced assessed by Folin–Ciocalteu method, DPPH assay, cellulo assays fibroblasts, such cell viability scratch migration gene expression analysis, immunofluorescence detection, visualization, quantification collagen-I, collagen-IIIa, elastin signals collagenase assay. Results: Treatment NHDFs combinatorial promoted collagen synthesis deposition normal conditions and, upon induced external stress, vitro transcriptomic proteomic analysis. A significant inhibition also observed, suggesting multitargeted active ingredients preventing degradation. Conclusions: Therefore, this liposome–cyclodextrin polyphenol complex represents novel bioactive ingredient promising applications.

Язык: Английский

The effect of extraction conditions on the chemical profile of obtained raw poplar propolis extract DOI Creative Commons
Michał Miłek, Radosław Bonikowski, Małgorzata Dżugan

и другие.

Chemical Papers, Год журнала: 2024, Номер 78(11), С. 6709 - 6720

Опубликована: Июнь 18, 2024

Abstract Various conditions of extraction were applied to obtain active extracts raw poplar-type propolis. The efficiency traditional maceration was evaluated in terms used solvent: ethanol (70 and 96% v/v), acetone (pure 70%), propylene glycol, glycerol (50%), water with modifiers: PEG 400 lecithin. For obtained extracts, the total amounts extracted phenolics flavonoids as well antioxidant activity compared. most profile volatile organic compounds use GC × GC–MS selected polyphenols content by HPLC–DAD To increase propolis, extract ultrasound microwave-assisted compared regarding main polyphenolic quantified HPLC method. recovery 70% ethanolic effect extension time also examined HPTLC Based on conducted analyses, found aromatic bioactive, followed pure polypropylene glycol extracts. Compared classic maceration, assisted microwaves ultrasounds did not provide a higher yield. In case extraction, 5-day duration is recommended which allows close 80% bioactive components

Язык: Английский

Процитировано

4
Pharmaceutical Insights Into Ammi and Parsley: Evaluating Antioxidant Activity, Total Phenolic Content, and Kidney Stone Disintegration Properties DOI Creative Commons
Ruba Malkawi, Khairat Battah, Mohammad Alkhreisat

и другие.

Advances in Pharmacological and Pharmaceutical Sciences, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

This study investigated the pharmaceutical potential of extracts from Ammi visnaga (Ammi) and Petroselinum crispum (Parsley), specifically focusing on their antioxidant activity, total phenolic content, efficacy in disintegrating calcium oxalate kidney stones. Parsley extracts, known for traditional medicinal uses, contain bioactive compounds with significant properties that have attracted attention research. Oxidative stress, a key factor various physiological disorders, underscores importance antioxidants mitigation cellular damage. Our investigation revealed concentration-dependent enhancements activity content both indicating as natural agents. Furthermore, were effective reducing size stones, extract demonstrating superior stone-disintegration properties. Dissolution studies provided valuable insights into release kinetics suggesting sustained therapeutic potential. Overall, show promise development, offering alternative avenues managing oxidative stress-related conditions stone formation.

Язык: Английский

Процитировано

0
Network Pharmacology and Experimental Validation Reveal Therapeutic Potential of Propolis in UV-Induced Allergic Dermatitis DOI Creative Commons

Liyuan Cheng,

Jie Wang, Y. Wang

и другие.

Foods, Год журнала: 2025, Номер 14(6), С. 996 - 996

Опубликована: Март 14, 2025

Propolis demonstrates diverse pharmacological properties encompassing antimicrobial, anti-inflammatory, antioxidant, immunomodulatory, and wound-healing activities. This study investigated the therapeutic mechanism of propolis against ultraviolet (UV)-induced allergic dermatitis through an integrated approach combining network pharmacology with in vitro experimental validation. The targets components were conducted PubChem, EMBL-EBI, SEA Search Server databases, disease-associated for atopic related conditions extracted from GeneCards. overlapping between UV-induced screened. Gene Ontology (GO) Enrichment analysis Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment performed. key further validated ELISA experiments using HSF cells. results show that there 28 dermatitis. GO 1246 terms biological functions, 52 cellular components, 98 molecular functions. KEGG obtained 110 signaling pathways. protein-protein interaction (PPI) showed TNF, NFKB1, MMP-9, IL-2 hub proteins. experiment confirmed reduced levels MMP-9 UBV-induced cells a dose-dependent manner. These findings provide mechanistic evidence supporting as promising functional food, dietary supplements, or medicinal agent skin disorders.

Язык: Английский

Процитировано

0
Recent developments and innovative application of propolis in the food industry: a natural preservative from honeybee waste DOI

Nalla Bhanu Prakash Reddy,

C. P. Indumathi,

Shweta Deotale

и другие.

Food Science and Biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Язык: Английский

Процитировано

0
Olive Mill Wastewater Extract: In Vitro Genotoxicity/Antigenotoxicity Assessment on HepaRG Cells DOI Open Access
Tommaso Rondini, Raffaella Branciari, Edoardo Franceschini

и другие.

International Journal of Environmental Research and Public Health, Год журнала: 2024, Номер 21(8), С. 1050 - 1050

Опубликована: Авг. 9, 2024

Olive mill wastewater (OMWW), with its high level of phenolic compounds, simultaneously represents a serious environmental challenge and great resource potential nutraceutical activities. To increase the knowledge OMWW's biological effects, an aim to developing food supplement, we performed chemical characterisation extract using Liquid Chromatography-Quadrupole Time-of-flight spectrometry (LC-QTOF) in vitro genotoxicity/antigenotoxicity assessment on HepaRG ™ cells. Chemical analysis revealed that most abundant compound was hydroxytyrosol. Biological tests showed not cytotoxic at lowest tested concentrations (from 0.25 2.5 mg/mL), unlike highest 5 20 mg/mL). Regarding genotoxic activity, when non-cytotoxic concentrations, did display any effect. Additionally, OMWW antigenotoxic activity (J-shaped dose-response effect) against known mutagenic substance, reducing extent DNA damage co-exposure treatment. The effect also obtained post-exposure procedure, although only 0.015625 0.03125 mg/mL. This behaviour confirmed pre-exposure protocol. In conclusion, present study established maximum non-toxic dose for cell model, smoothing path future research.

Язык: Английский

Процитировано

3
An Advanced Combinatorial System from Vitis vinifera Leaves and Propolis Enhances Antioxidants’ Skin Delivery and Fibroblasts Functionality DOI Creative Commons
Sophia Athanasopoulou, Eleni Spanidi, Eleni Panagiotidou

и другие.

Pharmaceuticals, Год журнала: 2024, Номер 17(12), С. 1610 - 1610

Опубликована: Ноя. 29, 2024

Background/Objectives: Vine leaves are a bulky by-product that disposed of and treated as waste in the wine production process. In present study polyphenols from vine were extracted simultaneously encapsulated new delivery system consisting liposomes cyclodextrins. This was further combined with propolis cyclodextrins, resulting colloidal suspension for release antioxidants time-controlled way, rate which depends on ratio materials. The result is raw material exhibits antioxidant ECM protective effects when administered skin fibroblasts (NHDFs). Methods: promoting efficacy produced assessed by Folin–Ciocalteu method, DPPH assay, cellulo assays fibroblasts, such cell viability scratch migration gene expression analysis, immunofluorescence detection, visualization, quantification collagen-I, collagen-IIIa, elastin signals collagenase assay. Results: Treatment NHDFs combinatorial promoted collagen synthesis deposition normal conditions and, upon induced external stress, vitro transcriptomic proteomic analysis. A significant inhibition also observed, suggesting multitargeted active ingredients preventing degradation. Conclusions: Therefore, this liposome–cyclodextrin polyphenol complex represents novel bioactive ingredient promising applications.

Язык: Английский

Процитировано

1