Evidence
has
revealed
that
oestrogen
deprivation-induced
osteolysis
is
microbiota-dependent
and
can
be
treated
by
probiotics.
However,
the
underlying
mechanism
require
further
investigation.
This
study
aims
to
provide
additional
evidence
supporting
use
of
probiotics
as
an
adjuvant
treatment
explore
pathophysiology
oestrogen-deprived
osteolysis.
Forty-five
SD
rats
were
randomly
divided
into
five
groups
(n
=
9).
Rats
from
four
ovariectomised
with
NS,
calcium,
probiotics,
or
calcium
+
while
one
group
underwent
a
sham
operation
was
NS.
The
osteometabolic
effects
evaluated,
mechanistic
role
probiotic
supplement
explored.
Intragastric
administration
Bifidobacterium
animalis
subsp.
lactis
LPL-RH
(LPL-RH)
markedly
suppressed
osteoclastic
activation
bone
loss
downregulating
TRAP
enzymatic
activity,
OPG/RANKL
ratio,
downstream
signalling
pathway
RANKL/TRAF6/NF-κB/NFATc1/TRAP
in
rats.
also
reduced
CD4+IL-17
A+
TH17
cells
marrow,
pro-osteoclastogenic
cytokine
IL-17
A,
pro-inflammatory
molecules
(LPS),
its
binding
protein
(LBP)
blood.
restored
intestinal
ZO-1,
occludin,
claudin
2,
12,
15,
which
improved
ileal
histopathology,
oxidative
stress,
attenuated
LPS-responsive
TLR4/MyD88/NF-κB
pathway.
Furthermore,
16
S
rRNA
sequencing
altered
faecal
microbiome
reducing
relative
abundance
S24-7
at
family
level
promoting
Prevotella
Bacteroides
genus
level.
Collectively,
osteoclastogenesis
modulating
type
17
immunity
gut
microbiome.
Journal of Food Science,
Год журнала:
2025,
Номер
90(2)
Опубликована: Фев. 1, 2025
Abstract
To
prepare
functional
yacon
juice,
this
study
investigates
the
optimal
fermentation
duration,
shelf‐life,
chemical
composition,
and
flavor
volatiles
of
juice
fermented
by
Lactiplantibacillus
plantarum
QS7T
capable
utilizing
fructo‐oligosaccharides
(FOS),
along
with
its
effects
on
gut
microbiota.
The
period
was
determined
as
30
h,
resulting
in
essential
parameters:
3.46
g
L
−1
for
total
acids
a
viable
lactic
acid
bacteria
(LAB)
count
2.3
×
10
8
CFU
mL
.
predicted
shelf
life
when
stored
at
4°C
is
28
days.
Throughout
storage,
maintained
significant
FOS
LAB
(8.0
7
).
Total
short‐chain
fatty
significantly
increased
post‐fermentation
reaching
177.86
µg
content
types
volatile
components
after
L.
increased,
thus
altering
improving
juice's
appeal.
Functional
also
induced
alterations
microbiota
structure
abundance
mice,
including
increasing
beneficial
microbes
such
Firmicutes,
Actinobacteriota,
Faecalibaculum,
Dubosiella,
Bifidobacterium,
Akkermansia
These
results
indicate
potential
food
modulation
anti‐inflammatory
benefits.
Microorganisms,
Год журнала:
2024,
Номер
12(7), С. 1350 - 1350
Опубликована: Июль 1, 2024
The
yak
(Bos
grunniens)
exhibits
exceptional
regional
adaptability,
enabling
it
to
thrive
in
the
distinctive
ecological
niches
of
Qinghai-Tibet
Plateau.
Its
survival
relies
on
intricate
balance
its
intestinal
microbiome,
essential
for
adapting
harsh
environmental
conditions.
Despite
documented
significance
bacteria
and
fungi
maintaining
homeostasis
supporting
immune
functions,
there
is
still
a
substantial
gap
understanding
how
composition
functionality
gut
microbiota
vary
along
altitude-temperature
gradients.
This
study
aims
fill
this
by
employing
16S
rRNA
ITS
amplicon
sequencing
techniques
analyze
compare
microbiome
yaks
residing
at
different
elevations
exposed
varying
temperatures.
findings
demonstrate
subtle
variations
diversity
fungi,
accompanied
significant
changes
taxonomic
across
various
altitudes
temperature
Notably,
Firmicutes,
Actinobacteriota,
Bacteroidota
emerged
as
dominant
phyla
all
groups,
with
Actinobacteriota
exhibiting
highest
proportion
(35.77%)
LZF
group.
Functional
prediction
analysis
revealed
associations
between
group
metabolic
pathways
related
amino
acid
metabolism
biosynthesis.
suggests
potential
role
actinomycetes
enhancing
nutrient
absorption
yaks.
Furthermore,
our
suggest
that
may
enhance
energy
catabolism
modulating
Firmicutes-to-Bacteroidota
ratio,
potentially
mitigating
effects
variations.
Variations
bacterial
fungal
communities
among
three
distinct
groups
were
analyzed
using
metagenomic
techniques.
Our
indicate
microbial
genera
increases
lower
are
largely
beneficial.
To
sum
up,
research
investigated
populations
diverse
altitude
ranges.
Moreover,
these
results
comprehension
makeup
variability,
offering
perspectives
resilience
dry
lot
feeding
from
angle.
Nutrients,
Год журнала:
2025,
Номер
17(6), С. 998 - 998
Опубликована: Март 12, 2025
Background:
High-altitude
hypoxia
is
known
to
adversely
affect
bone
health,
leading
accelerated
loss
and
metabolic
alterations.
Recent
studies
suggest
that
factors
such
as
bicarbonate
gut
microbiota
may
play
key
roles
in
health.
Mineral
water,
rich
bicarbonate,
influence
health
the
gut–bone
axis
under
conditions.
Methods:
Mice
were
exposed
treated
with
different
concentrations
of
drinking
water.
Bone-related
parameters
assessed
using
dual-energy
X-ray
absorptiometry
(DXA)
Micro-CT.
Bone
was
measurement
serum
biomarkers.
Additionally,
Untargeted
Metabolomics
employed
analyze
differential
metabolites
between
groups,
while
composition
analyzed
16S
rRNA
sequencing.
Results:
BMW
consumption
increased
mineral
density
(BMD)
helped
alleviate
damage
microstructure
bones
caused
by
delayed
progression
osteoporosis.
shown
enhance
probiotics
Akkermansia
Dubosiella
regulate
longevity-regulating
pathway
well
PI3K/AKT/mTOR
(PAM)
signaling
pathway.
This
study
also
discovered
changes
products
due
intervention,
predominantly
pathways
amino
acid,
prostaglandin,
purine
metabolisms,
correlation
analysis
further
exploring
relationships
these
metabolites.
Conclusions:
Long-term
exposure
high-altitude
hypoxic
conditions
affects
structure
metabolism
mice.
The
improves
regulates
maintain
hypoxia.
