Biomedical Optics Express,
Год журнала:
2024,
Номер
15(12), С. 6893 - 6893
Опубликована: Ноя. 5, 2024
Full
automation
of
single-molecule
localization
microscopy
(SMLM)
is
crucial
for
large-scale
and
high-throughput
cellular
imaging.
It
well-known
that
SMLM
typically
consists
three
major
steps:
immunofluorescence
(IF)
staining,
optical
imaging,
image
processing.
Currently,
in
imaging
processing
almost
complete;
however,
the
IF
staining
has
been
slow
to
advance,
probably
due
its
complicated
experimental
operations.
Here
we
present
a
low-cost
automated
method
called
super-resolution
by
microfluidics
(SRIF-fluidics).
This
suitable
both
adherent
suspension
cells
supports
single-color
multi-color
SMLM.
Our
results
show
SRIF-fluidics
reduces
antibody
consumption
about
75%
shortens
sample
preparation
time
from
5.6
hours
(manual
operation)
2.5
∼
4.4
hours,
depending
on
types.
Importantly,
this
provides
satisfactory
consistency
without
sacrificing
labeling
quality.
We
believe
proposed
paper
necessary
supplement
achieving
fully
facilitating
near
future.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Янв. 29, 2024
With
the
recent
success
of
lipid
nanoparticle
(LNP)
based
SARS-CoV-2
mRNA
vaccines,
potential
for
RNA
therapeutics
has
gained
widespread
attention.
LNPs
are
promising
non-viral
delivery
vectors
to
protect
and
deliver
delicate
therapeutics,
which
ineffective
susceptible
degradation
alone.
While
food
drug
administration
(FDA)
approved
formulations
have
shown
significant
promise,
benchmark
still
require
optimization
improvement.
In
addition,
translatability
these
several
different
cargo
sizes
not
been
compared
under
same
conditions.
Herein
we
analyze
"gold
standard"
encapsulation
efficiency
various
non-specific
lengths
representing
antisense
oligonucleotides
(ASO),
small
interfering
(siRNA),
aptamers,
messenger
(mRNA),
with
10
bases,
21
base
pairs,
96
996
1929
respectively.
We
evaluate
as
percentage
input
encapsulated
in
final
LNP
product
(EE
Advanced Materials,
Год журнала:
2024,
Номер
36(27)
Опубликована: Март 29, 2024
Cancer
treatment
requires
precise
tumor-specific
targeting
at
specific
sites
that
allows
for
high-resolution
diagnostic
imaging
and
long-term
patient-tailorable
cancer
therapy;
while,
minimizing
side
effects
largely
arising
from
non-targetability.
This
can
be
realized
by
harnessing
exogenous
remote
stimuli,
such
as
tissue-penetrative
ultrasound,
magnetic
field,
light,
radiation,
enable
local
activation
therapy
in
deep
tumors.
A
myriad
of
nanomedicines
efficiently
activated
when
the
energy
stimuli
transformed
into
another
type
energy.
review
discusses
control
transformation
targetable,
efficient,
therapy.
Such
ultrasonic,
magnetic,
photonic,
radiative,
radioactive
mechanical,
thermal,
chemical,
radiative
to
a
variety
modalities.
The
current
article
describes
multimodal
where
serial
cascade
or
multiple
types
occur.
includes
not
only
hyperthermia,
radiation
but
also
emerging
thermoelectric,
pyroelectric,
piezoelectric
therapies
treatment.
It
illustrates
resonance,
fluorescence,
computed
tomography,
photoluminescence,
photoacoustic
imaging-guided
therapies.
highlights
afterglow
eliminate
autofluorescence
sustained
signal
emission
after
excitation.
Journal of Composites Science,
Год журнала:
2024,
Номер
8(10), С. 386 - 386
Опубликована: Сен. 25, 2024
The
inception
of
microfluidic
devices
marks
a
confluence
diverse
scientific
domains,
including
physics,
biology,
chemistry,
and
fluid
mechanics.
These
multidisciplinary
roots
have
catalyzed
the
evolution
devices,
which
serve
as
versatile
platforms
for
various
chemical
biological
processes.
Notably,
garnered
attention
efficient
reactors,
offering
distinct
benefits
such
minimized
spatial
requirements
reactions,
reduced
equipment
costs,
accelerated
residence
times.
advantages,
among
others,
ignited
compelling
interest
in
harnessing
technology
conception,
refinement,
production
nanomaterials
nanocomposites,
pivotal
within
both
industrial
medicinal
sectors.
This
comprehensive
exposition
delves
into
multifaceted
aspects
nanomaterial
synthesis,
underscoring
transformative
role
methodologies
departure
from
conventional
techniques.
discourse
navigates
through
intricate
considerations
surrounding
preparation
nanomaterials,
elucidating
how
paradigm
has
emerged
promising
alternative.
paper
serves
an
illuminating
exploration
juncture
between
innovation
synthesis.
It
traverses
potential
microfluidics
revolutionizing
traditional
approaches,
heralding
new
era
precision
engineering
advanced
materials
with
applications
spanning
to
domains.
Journal of Nanotechnology and Nanomaterials,
Год журнала:
2022,
Номер
3(2)
Опубликована: Окт. 6, 2022
In
nature,
nanoparticles
can
be
found
as
natural
or
synthetic.
Nanoparticles
may
form
either
physically
chemically.
are
uniform
types
of
common
material
whose
dimensions
less
than
100
nm.
Volcanic
ash,
ocean
spray,
fine
sand,
and
dust
some
examples
nanoparticles.
Synthetic
nanoparticles,
also
known
anthropogenic
engineered
that
specifically
designed
to
study
various
physiochemical
features
for
research
purposes
Biosensors,
Год журнала:
2025,
Номер
15(4), С. 220 - 220
Опубликована: Март 29, 2025
Circulating
tumor
cells
(CTCs)
are
vital
indicators
of
metastasis
and
provide
a
non-invasive
method
for
early
cancer
diagnosis,
prognosis,
therapeutic
monitoring.
However,
their
low
prevalence
heterogeneity
in
the
bloodstream
pose
significant
challenges
detection.
Microfluidic
systems,
or
“lab-on-a-chip”
devices,
have
emerged
as
revolutionary
tool
liquid
biopsy,
enabling
efficient
isolation
analysis
CTCs.
These
systems
offer
advantages
such
reduced
sample
volume,
enhanced
sensitivity,
ability
to
integrate
multiple
processes
into
single
platform.
Several
microfluidic
techniques,
including
size-based
filtration,
dielectrophoresis,
immunoaffinity
capture,
been
developed
enhance
CTC
The
integration
machine
learning
(ML)
with
has
further
improved
specificity
accuracy
detection,
significantly
advancing
speed
efficiency
diagnosis.
ML
models
enabled
more
precise
CTCs
by
automating
detection
enhancing
identify
rare
heterogeneous
cell
populations.
advancements
already
demonstrated
potential
improving
diagnostic
personalized
treatment
approaches.
In
this
review,
we
highlight
latest
progress
technologies
algorithms,
emphasizing
how
combination
changed
diagnosis
contributed
field.
Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 3, 2025
Abstract
Surface
modification
of
nanoparticles
(NPs)
via
the
layer‐by‐layer
(LbL)
technique
is
a
promising
approach
to
generate
targeted
drug
delivery
vehicles.
LbL‐NPs
have
been
successfully
used
in
preclinical
models
for
controlled
release,
tumor
and
immune
cell
targeting,
improved
pharmacokinetics
biodistribution,
controlling
cellular
trafficking
uptake
mechanisms.
A
simple
scalable
synthesis
method
that
can
be
adapted
clinical
translation
great
interest.
Here
new
polymer
deposition
presented
onto
NPs
enabled
through
microfluidic
(MCF)
mixing.
are
mixed
with
polyelectrolytes
using
commercially
available
bifurcating
mixer
MCF
cartridges.
In
addition
increased
process
robustness,
allows
LbL
electrostatic
assembly
titrated
polymer‐to‐NP
weight
equivalent
ratios
where
no
excess
required
achieve
given
layering.
Under
such
conditions,
time‐consuming
purification
needed,
greatly
increasing
LbL‐NP
throughput
avoiding
loss
during
purification.
The
utility
this
system
demonstrated
interleukin‐12‐loaded
liposomal
NPs,
which
show
efficacy
vitro
vivo
generated
traditional
lab‐scale
batch‐wise
adsorption
tangential
flow
filtration
Moreover,
it
shown
assemble
films
various
chemistries
on
NP
core
substrates.
